Search results for "EOS"
showing 10 items of 2714 documents
Postmortem Electrical Conductivity Changes of Dicentrarchus labrax Skeletal Muscle: Root Mean Square (RMS) Parameter in Estimating Time since Death
2022
Electric impedance spectroscopy techniques have been widely employed to study basic biological processes, and recently explored to estimate postmortem interval (PMI). However, the most-relevant parameter to approximate PMI has not been recognized so far. This study investigated electrical conductivity changes in muscle of 18 sea bass specimens, maintained at different room temperatures (15.0 °C; 20.0 °C; 25.0 °C), during a 24 h postmortem period using an oscilloscope coupled with a signal generator, as innovative technology. The root mean square (RMS) was selected among all measured parameters, and recorded every 15 min for 24 h after death. The RMS(t) time series for each anima…
All-atom simulations disentangle the functional dynamics underlying gene maturation in the intron lariat spliceosome
2018
The spliceosome (SPL) is a majestic macromolecular machinery composed of five small nuclear RNAs and hundreds of proteins. SPL removes noncoding introns from precursor messenger RNAs (pre-mRNAs) and ligates coding exons, giving rise to functional mRNAs. Building on the first SPL structure solved at near–atomic-level resolution, here we elucidate the functional dynamics of the intron lariat spliceosome (ILS) complex through multi-microsecond-long molecular-dynamics simulations of ∼1,000,000 atoms models. The ILS essential dynamics unveils (i) the leading role of the Spp42 protein, which heads the gene maturation by tuning the motions of distinct SPL components, and (ii) the critical particip…
Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells.
2002
BACKGROUND Gap-junction-mediated intercellular communication (GJIC) is required for normal development and tissue homeostasis. However, the role of GJIC in human prostate carcinogenesis and progression remains ill-defined. METHODS The ability of hormones, anti-hormones, and the anti-hypertensive drug, forskolin, to restore GJIC in non-tumorigenic (RWPE-1 and PWR-1E) and malignant (RWPE-2, LNCaP, DU-145) human prostate epithelial cell lines, was examined by Scrape-Loading/Dye Transfer (SL/DT) and Fluorescence Recovery After Photobleaching (FRAP) methods using an Ultima laser cytometer. RESULTS Results from both assays show that PWR-1E, RWPE-2, LNCaP, and DU-145 cells have weak or absent GJIC…
Isolated osteoblastoma of the cuboid bone: A case report and review of the literature
2020
Osteoblastoma is a relatively rare, benign, bone-forming tumor, commonly observed in the second and third decades of life. Spine and the long tubular bones are the most common sites of involvement. Osteoblastoma is infrequently seen in other sites, including the bones of hand and foot. A rare case of a 35-year-old man that presented an osteoblastoma of the cuboid bone is reported. The patient was treated with surgical resection and grafting. After the intervention, the patient recovered with no clinical and radiological evidence of recurrence after one year of follow-up. Several cases of osteoblastoma-like variant of osteosarcoma of the cuboid have been previously reported, but, to our know…
The effect of 3-aminobenzamide, inhibitor of poly(ADP-ribose) polymerase, on human osteosarcoma cells
2003
This study demonstrates that in human osteosarcoma cells treatment with 3-aminobenzamide (3-AB), a potent inhibitor of poly(ADP-ribose) polymerase (PARP), induces morphological and biochemical features of differentiation, the duration of which depends on whether or not the normal RB gene is expressed. In Saos-2 cells expressing a non-functional Rb protein, 3-AB treatment induced the formation of transient, short dendritic-like protrusions. In RB-transfected-Saos-2 cells (a clone previously generated in our laboratory that shows stable expression of wild-type Rb protein), 3-AB induced marked and prolonged changes with the formation of long dendritic-like protrusions and the appearance of ste…
Plio-Pleistocene Dust Traps on Paleokarst Surfaces: A Case Study From the Carpathian Basin
2020
Plio-Pleistocene silt/clay-rich deposits and paleo-karst fissure sediments from sites of the northern and southern parts of the Carpathian Basin were investigated. These materials were supposed to be mixed during transport before being captured in karstified fissures. Evidence that the eolian fissure sediments of Plio-Pleistocene age in the older Triassic–Cretaceous limestones are derived from eolian silt and clay includes compositional and textural matches, especially decreasing grain-size trends observed downwards from the paleo-surface of the former landscape. Various environmental factors could be recognized by the statistical evaluation of grain-size distribution curves of fissure fill…
Mapping and determinism of soil microbial community distribution across an agricultural landscape.
2015
Article en open access; International audience; Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13km(2). Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution…
Comprehensive analysis of interacting proteins and genome-wide location studies of the Sas3-dependent NuA3 histone acetyltransferase complex
2014
Highlights • We characterise Sas3p and Gcn5p active HAT complexes in WT and deleted TAP-strains. • We confirm that Pdp3p interacts with NuA3, histones and chromatin regulators. • Pdp3p MS-analysis reveals its phosphorylation, ubiquitination and methylation. • Sas3p can substitute Gcn5p in acetylation of histone H3K14 but not of H3K9. • Genome-wide profiling of Sas3p supports its involvement in transcriptional elongation.
Geostatistical techniques for runoff mapping: an application to Sicily, Italy
2011
The availability of reliable and long time series of runoff data is fundamental for most of the hydrological analyses and for the assessment and the management of water resources even in condition of global climatic change. However, hydrologic data sets are often characterized by a short duration and also suffer from missing data values, mainly due to malfunctioning of gauging stations for a specific period. In order to overcome this problem and obtain long and continuous runoff time series, different models and methods have been previously developed and proposed. While some models, used to extent the streamflow record, are conceptual, empirical, regressive models based on the rainfall inpu…
<p class="Body"><strong>Biological parameters of <em>Neoseiulus longilaterus</em> (Athias-Henriot) (Parasitiformes, Phytoseii…
2019
Tetranychus urticae is the most important pest on different crops worldwide. Search for endemic phytoseiid mites co-occurring with the above pest, which might be good candidates as biocontrol agents, represents the policy followed by most scientists in recent years. The aim of the present work was to assess the various biological and demographic parameters of Neoseiulus longilaterus, associated with T. urticae on weeds, in laboratory conditions using the latter tetranychid and pollen of Oxalis pes-caprae as food. The results showed a better performance of the predator on the prey as regards the postembryonic development (7.63 and 8.18 days for prey and pollen respectively) and the demograph…