Search results for "EOT"

Molecular biomass and MetaTaxogenomic assessment of soil microbial communities as influenced by soil DNA extraction procedure

2011

Three soil DNA extraction procedures (homemade protocols and commercial kit) varying in their practicability were applied to contrasting soils to evaluate their efficiency in recovering: (i) soil DNA and (ii) bacterial diversity estimated by 16S rDNA pyrosequencing. Significant differences in DNA yield were systematically observed between tested procedures. For certain soils, 10 times more DNA was recovered with one protocol than with the others. About 15,000 sequences of 16S rDNA were obtained for each sample which were clustered to draw rarefaction curves. These curves, as well as the PCA ordination of community composition based on OTU clustering, did not reveal any significant differenc…

Crop Yield Estimation and Interpretability With Gaussian Processes

2021

This work introduces the use of Gaussian processes (GPs) for the estimation and understanding of crop development and yield using multisensor satellite observations and meteo- rological data. The proposed methodology combines synergistic information on canopy greenness, biomass, soil, and plant water content from optical and microwave sensors with the atmospheric variables typically measured at meteorological stations. A com- posite covariance is used in the GP model to account for varying scales, nonstationary, and nonlinear processes. The GP model reports noticeable gains in terms of accuracy with respect to other machine learning approaches for the estimation of corn, wheat, and soybean …

Intercultural crisis communication during the 2011 Fukushima nuclear disaster

2014

With the motivation of contributing to further development of the emerging field of intercultural crisis communication, the study aims at exploring the reasons why the global media criticized the Japanese authorities’ crisis communication during the 2011 Fukushima nuclear disaster through the inductive content analysis on both the Japanese authorities’ crisis communication and the global media’s perceptions on the Japanese authorities’ crisis communication. The data collected from 11 March to September 2011, including 160 press releases, transcripts of the foreign press conferences and speeches delivered at the international conferences by the Japanese authorities, and 120 news reports from…

Pandemic Spread of COVID-19 Mutant Variants Will Facilitate Next-generation Sequencing Capacities for Personalised Medicine in Urologic Oncology.

2021

Horses Could Perceive Riding Differently Depending on the Way They Express Poor Welfare in the Stable

2020

International audience; This study investigated the relationships between four behavioral and postural indicators of a compromised welfare state in loose boxes (stereotypies, aggressive behaviors toward humans, withdrawn posture reflecting unresponsiveness to the environment, and alert posture indicating hypervigilance) and the way horses perceived riding. This perception was inferred using a survey completed by the usual riding instructor and during a standardized riding session (assessment of behaviors and postures, qualitative behavior assessment (QBA) and characterization of the horses' locomotion using an inertial measurement unit). In accordance with ear and tail positions and the QBA…

Genetic and Epigenetic Factors of Takotsubo Syndrome: A Systematic Review

2021

Takotsubo syndrome (TTS), recognized as stress’s cardiomyopathy, or as left ventricular apical balloon syndrome in recent years, is a rare pathology, described for the first time by Japanese researchers in 1990. TTS is characterized by an interindividual heterogeneity in onset and progression, and by strong predominance in postmenopausal women. The clear causes of these TTS features are uncertain, given the limited understanding of this intriguing syndrome until now. However, the increasing frequency of TTS cases in recent years, and particularly correlated to the SARS-CoV-2 pandemic, leads us to the imperative necessity both of a complete knowledge of TTS pathophysiology for identifying bi…

Analytical solution of kinematic wave time of concentration for overland flow under green-ampt infiltration

2015

In this paper the well-known kinematic wave equation for computing the time of concentration for impervious surfaces has been extended to the case of pervious hillslopes, accounting for infiltration. An analytical solution for the time of concentration for overland flow on a rectangular plane surface is derived using the kinematic wave equation under the Green-Ampt infiltration. The relative time of concentration is defined as the ratio between the time of concentration of an infiltrating plane and the soil sorptivity time scale, depending on the normalized rainfall intensity and a parameter synthesizing the soil and hillslope characteristics. It is shown that for a more complex case (corre…

Partial purification and characterization of an NAD-dependent 3 beta-hydroxysteroid dehydrogenase from Clostridium innocuum

1989

In nine strains of Clostridium innocuum, 3 beta-hydroxysteroid-dehydrogenating activities were detected. 3 beta, 7 alpha, 12 alpha-Trihydroxy- and 3 beta-hydroxy-12-keto-5 beta-cholanoic acids were identified as reduction products of the respective 3-keto bile acids by gas-liquid chromatography and gas-liquid chromatography-mass spectrometry. One strain was shown to contain a NAD-dependent 3 beta-hydroxysteroid dehydrogenase. Enzyme production was constitutive in the absence of added bile acids. The specific enzyme activity was significantly reduced by growth medium supplementation with 3-keto bile acids, with trisubstituted acids being more effective than disubstituted ones. A pH optimum o…

Exploiting Low-Cost 3D Imagery for the Purposes of Detecting and Analyzing Pavement Distresses

2020

Road pavement conditions have significant impacts on safety, travel times, costs, and environmental effects. It is the responsibility of road agencies to ensure these conditions are kept in an acceptable state. To this end, agencies are tasked with implementing pavement management systems (PMSs) which effectively allocate resources towards maintenance and rehabilitation. These systems, however, require accurate data. Currently, most agencies rely on manual distress surveys and as a result, there is significant research into quick and low-cost pavement distress identification methods. Recent proposals have included the use of structure-from-motion techniques based on datasets from unmanned a…

Integrated CGH/WES Analyses Advance Understanding of Aggressive Neuroblastoma Evolution: A Case Study

2021

Neuroblastoma (NB) is the most common extra-cranial malignancy in preschool children. To portray the genetic landscape of an overly aggressive NB leading to a rapid clinical progression of the disease, tumor DNA collected pre- and post-treatment has been analyzed. Array comparative genomic hybridization (aCGH), whole-exome sequencing (WES), and pharmacogenetics approaches, respectively, have identified relevant copy number alterations (CNAs), single nucleotide variants (SNVs), and polymorphisms (SNPs) that were then combined into an integrated analysis. Spontaneously formed 3D tumoroids obtained from the recurrent mass have also been characterized. The results prove the power of combining C…