Search results for "EXOSOMES"

Extracellular vesicles shuffling intercellular messages: for good or for bad

2015

The release of extracellular vesicles (EVs) is a highly conserved process exploited by diverse organisms as a mode of intercellular communication. Vesicles of sizes ranging from 30 to 1000. nm, or even larger, are generated by blebbing of the plasma membrane (microvesicles) or formed in multivesicular endosomes (MVEs) to be secreted by exocytosis as exosomes. Exosomes, microvesicles and other EVs contain membrane and cytosolic components that include proteins, lipids and RNAs, a composition that differs related to their site of biogenesis. Several mechanisms are involved in vesicle formation at the plasma membrane or in endosomes, which is reflected in their heterogeneity, size and composit…

Exosome Involvment in Hsp60 secretion by tumor cells.

2011

Exo-ALK Proof of Concept: Exosomal Analysis of ALK Alterations in Advanced NSCLC Patients

2017

Effects of exosomes released by NSCLC cells on osteoclasts differentiation in ISTITUTO SUPERIORE DI SANITA’, ROMA AICC

2016

Heat Shock Proteins exosomal localization and levels in non-tumoral and tumoral thyroid tissues

Exosomes (EXs) secreted from cells to the extracellular environment play an important role in intercellular communication in normality and pathology. In particular, EXs contribute to cancer progression through the modulation of tumor microenvironment. Heat Shock Proteins (Hsps) are implicated in the development of carcinogenic process because they interact with many metabolic and biomolecular mechanisms of cancer cells [1]. Different studies shown that they can be released by tumors cells and that the mechanism of release is mediated by EXs pathway. In this study we performed an immunomorphological and biomolecular analysis to investigate Hsp27, Hsp60, Hsp70, Hsp90 levels expression profile…

Exosomes analysis in Non Small Cell Lung Cancer: from in vitro models to preclinical application

Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related deaths worldwide. The majority of patients are diagnosed in advanced disease stage. Bone metastasis is the most frequent complication in NSCLC resulting in osteolytic lesions. The perfect balance between bone-resorbing osteoclasts and bone-forming osteoblasts activity is lost in bone metastasis, inducing osteoclastogenesis. In NSCLC, the epidermal growth factor receptor (EGFR) pathway is constitutively activated. EGFR binds Amphiregulin (AREG) that is overexpressed in several cancers such as colon, breast and lung. Its levels in plasma of NSCLC patients correlate with poor prognosis and AREG was recently found as…

EXOSOMES: CAN DOCTORS STILL IGNORE THEIR EXISTENCE?

2013

With this invited commentary we want to draw the attention of young medical doctors, the main readers of this journal, towards the existence and importance of a group of nanovesicles released by human cells: the exosomes. These vesicles are incontinently se-creted as a mean of cell-to-cell communication. They are involved in a number of physiol-ogic processes as well as in the pathogenesis of, virtually, all human diseases. They can be isolated from all biological fluids, like blood, urine, sweat, sperm, crevicular fluid, bile, etc., and their composition in terms of proteins, RNA and lipids is different in pathology that in physiologic conditions. It is therefore possible to predict that t…

Exosomes released by k562 chronic myeloid leukemia cells promote endothelial cell tubular differentiation through uptake and cell-to-cell transfer

2012

Exosomes, microvesicles of endocytic origin released by normal and tumor cells, play an important role in cell-to-cell ommunication. Angiogenesis has been shown to regulate progression of chronic myeloid leukemia (CML). The mechanism through which this happens has not been elucidated. We isolated and characterized exosomes from K562 CML cells and evaluated their effects on human umbilical endothelial cells (HUVECs). Fluorescent-labeled exosomes were nternalized by HUVECs during tubular differentiation on Matrigel. Exosome localization was perinuclear early in differentiation, moving peripherally in cells undergoing elongation and connection. Exosomes move within and between nanotubular stru…

Molecular profiling and function of exosomes derived from Non-Small Cell Lung Cancer. Diagnostic, predictive and prognostic biomarkers.

2019

Abstract: abstract not available

Curcumin modulates chronic myelogenous leukemia exosomes composition and affects angiogenic phenotype, via exosomal miR-21.

2016

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