Search results for "EXPA"
showing 10 items of 820 documents
Comparison of British and French expatriate doctors’ characteristics and motivations
2012
Objective The aim of this study was to analyse the migration of doctors between the UK and France, in an attempt to identify the reasons for these migrations. Design This was a cross-sectional study conducted using a self-completed questionnaire. Setting The questionnaire was sent to all British doctors practising in France and to all French doctors practising in the UK. Participants The doctors were identified, thanks to official data of the National Medical Councils. There were 244 French doctors practising in the UK and 86 British doctors practising in France. Outcome measures A questionnaire was specifically developed for the study to determine the reasons why doctors moved to the other…
Nouvelles données sur le genre Bragasellus (Crustacea: Isopoda: Asellidae
1996
From now on, the genus Bragasellus Henry & Magniez, 1968 includes 2 oculated and 17 stygobiotic species. As a natural and monophyletic taxonomic unit, we consider it a good genus. Its original area corresponds to the north-west quarter of the Iberian Peninsula. Secondarily, this area has extended eastward, using mainly the alluvial channels of hydrographic systems (Ríos Douro + Ebro and tributaries), finally reaching the underground waters of several Mediterranean rivers. This active expansion is exclusively due to the migration of two stygobiotic sibling species: B. lagari Henry & Magniez, 1973 towards the high basin of the Río Tajo, then downstream to the basins of the Ríos Jucar and Turi…
Acculturation of foreign IT workers in Japan from a cognitive and business management viewpoint
2016
This dissertation investigates expatriate IT workers located in Japan in the contexts of their acculturation and thinking about workplace and business negotiations. Case studies of individual actors supported by surveys were chosen as the methods to gather data leading to findings about how expatriates develop in Japan, including their ability to adjust, accept, and reject schemata about business management situations. Individuals were chosen as a unit of study because they are the key figures who decide the economic fate of companies. Schemata were chosen as a study focus in the later articles because they are the cognitive location of information about home and host culture and come into …
Preclinical characterization of antagomiR-218 as a potential treatment for myotonic dystrophy
2021
Myotonic dystrophy type 1 (DM1) is a rare neuromuscular disease caused by expansion of unstable CTG repeats in a non-coding region of the DMPK gene. CUG expansions in mutant DMPK transcripts sequester MBNL1 proteins in ribonuclear foci. Depletion of this protein is a primary contributor to disease symptoms such as muscle weakness and atrophy and myotonia, yet upregulation of endogenous MBNL1 levels may compensate for this sequestration. Having previously demonstrated that antisense oligonucleotides against miR-218 boost MBNL1 expression and rescue phenotypes in disease models, here we provide preclinical characterization of an antagomiR-218 molecule using the HSALR mouse model and patient-d…
Expression and purification of polyhistidine-tagged firefly luciferase in insect cells
2001
The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and purified by immobilized metal-ion affinity chromatography in combination with an expanded bed adsorption system. This approach enabled an efficient, one-step purification protocol of a genetically modified luciferase with properties similar to those of the authentic counterpart. According to light emission measurements, the final yield of highly purified protein was 23 mg l−1 of cell culture. In addition, no specific interaction of interfering …
Changes in the Phytochemical Profile and Antioxidant Properties of Prunus persica Fruits after the Application of a Commercial Biostimulant Based on …
2022
Plant biostimulants are formulations that are experiencing great success from the perspective of sustainable agriculture. In this work, we evaluated the effect derived from the application of a biostimulant based on algae and yeast extracts (Expando®) on the agronomic yield and nutraceutical profile of two different cultivars (“Sugar Time” and “West Rose”) of Prunus persica (peach). Although, at the agronomic level, significant effects on production yields were not recorded, the biostimulant was able to reduce the ripening time, increase the fruit size, and make the number of harvestable fruits homogeneous. From a nutraceutical point of view, our determinations v…
Lag time for germination of Penicillium chrysogenum conidia is induced by temperature shifts.
2013
In the environment, fungal conidia are subject to transient conditions. In particular, temperature is varying according to day/night periods. All predictive models for germination assume that fungal spores can adapt instantaneously to changes of temperature. The only study that supports this assumption (Gougouli and Koutsoumanis, 2012, Modelling germination of fungal spores at constant and fluctuating temperature conditions. International Journal of Food Microbiology, 152: 153-161) was carried out on Penicillium expansum and Aspergillus niger conidia that, in most cases, already produced germ tubes. In contrast, the present study focuses on temperature shifts applied during the first stages…
Climate Change Effects on Agricultural Insect Pests in Europe
2015
Abstract In this chapter, we will discuss observations of climate change effects on agricultural pests in Europe, the possible mechanisms behind these observed effects and finally delve into more detail through some relatively well studied model species (the Colorado potato beetle and the rape beetle). Direct effects of climate change on agricultural pests in Europe are difficult to dissect from all the human-induced changes that have taken place in parallel with an increased mean annual temperature. During the past decade, agriculture has become more professionalized in terms of land use, crop cultivation techniques and pest management strategies. We review the effect of climate change on …
Autonomy, Heterogeneity and Trust in Mobile P2P environments
2007
Mobile P2P environments are emerging as a result of rapid expansion of portable terminals that are able to establish direct wireless communication links among themselves. These kinds of terminals are under the control of persons and allowing interactions between their terminals is subject to trust between the individuals and their desire to preserve various aspects of autonomy against each other. In this paper we relate the concepts of node autonomy, heterogeneity and trust with each other, defining also numeric measures for them. We also investigate what kind of interactions (such as transactions, file exchanges) are possible at certain heterogeneity, autonomy and trust level between the t…
C9ORF72 in a Large Series of Italian and Sardinian Familial and Sporadic ALS Patients (IN9-1.003)
2012
Objective: To assess the frequency and the phenotype of a large series of Italian sALS and fALS with C9ORF72 repeat expansions. Background Recently we found that large expansions of hexanucleotide repeats (GGGGCC) in the first intron of the C9ORF72 gene, located in the chromosome 9p21, are related to familial and sporadic ALS cases(Renton et al, 2011). Design/Methods: We assessed 126 index fALS (106 Italians, 20 of Sardinians) and 601 sALS (485 Italians, 116 Sardinians), negative for other ALS-related genes mutations. Patients were collected through the ITALSGEN consortium. Repeat primer PCR to screen the presence of the hexanucleotide expansion in the first intron of C9ORF72 have been perf…