Search results for "EXPRESSION"
showing 10 items of 5168 documents
Single-cell RNA sequencing unveils the shared and the distinct cytotoxic hallmarks of human TCRVδ1 and TCRVδ2 γδ T lymphocytes
2019
γδ T lymphocytes represent ∼1% of human peripheral blood mononuclear cells and even more cells in most tissues of vertebrates. Although they have important anticancer functions, most current single-cell RNA sequencing (scRNA-seq) studies do not identify γδ T lymphocytes because their transcriptomes at the single-cell level are unknown. Here we show that high-resolution clustering of large scRNA-seq datasets and a combination of gene signatures allow the specific detection of human γδ T lymphocytes and identification of their T cell receptor (TCR)Vδ1 and TCRVδ2 subsets in large datasets from complex cell mixtures. In t -distributed stochastic neighbor embedding plots from blood and tumor sa…
Visual exploration of face and facial expression in infancy: A qualitative approach of cognitive and social development
2016
International audience; This article proposes a methodological consideration for the use of "head free" eye-tracking systems, which allowed to extend this technique to the study of infant skills. It explores how the technological developments enable a more qualitative approach, which offers the possibility of considering "how" in addition to "how long" the infant looks at a visual scene, especially the scene of the face.
Study of biogenic amines metabolism in wine lactic acid bacteria
2011
Biogenic amines are indesirable compounds found in fermented products like wine. Lactic acid bacteria from wine, including Oenococcus oeni, the main actor of malolactic fermentation, are able to produce these molecules from nitrogenous precursors. In order to limited biogenic amines accumulation, it is necessary to understand the role of this production by strains responsible for the synthesis of these metabolites in food. That is why the European BiamFood project was put in place. Along my thesis, molecular tools were developed in order to mutate O. oeni genes (encoding decarboxylases), and to express genes of interest. Genetic clusters hdc from L. higardii and odc from O. oeni, responsibl…
Communication and Autoinduction in the species Listeria monocytogenes
2014
International audience; In order to withstand changes in their environment, bacteria have evolved mechanisms to sense the surrounding environment, integrate these signals and adapt their physiology to thrive under fluctuating conditions. Among these mechanisms, the ability of bacteria to exchange information between cells has become a dynamic field of interest for microbiologists over the past four decades. First described by Nelson et al.,1 this phenomenon often referred as either cell-cell communication, Quorum Sensing and/or AutoInduction involves the synthesis of small signal molecules called autoinducers. These signal molecules may be sensed by the bacterial population in the vicinity …
Characterization of Pea (Pisum Sativum L.) genes implicated in arbuscular mycorrhiza formation and function
2010
The arbuscular mycorrhizal (AM) association results from a successful interaction between the genomes of the two symbiotic partners. In this context, the aim of my research was to better characterize the role of the late stage symbiosis-related pea genes PsSym36, PsSym33 and PsSym40 in the functional AM (i) by investigating the effect of mutations in the three genes on fungal and plant gene responses and (ii) by creating conditions for the localization of two of the genes, PsSym36 and PsSym40, on the pea genetic map for future map-based cloning. The expression of a subset of ten fungal and eight plant genes,previously reported to be activated during mycorrhiza development, was compared in G…
Localisation et visualisation de transcrits fongiques in situ: Une méthode originale qui combine biologie moléculaire et microscopie confocale
2011
National audience; Les Gloméromycètes, formant la symbiose mycorhizienne à arbuscules, sont des champignons biotrophes obligatoires qui sont intimement associés aux tissus végétaux qu’ils colonisent. Les techniques utilisées pour suivre l’expression des gènes fongiques dans les racines mycorhizées ne permettent pas de définir le profil spatio-temporel de leur activité. Afin de tracer l’activité transcriptionnelle fongique dans les racines mycorhizées, une méthode innovante, basée sur la RT-PCR in situ, a donc été développée. Cette technique permet de localiser, en microscopie optique confocale à balayage laser, des transcrits fongiques, à l’aide d’amorces spécifiques marquées par un fluoroc…
Genes encoding transcription factors in Glomus intraradices and their expression at the appressoria stage of arbuscular mycorrhiza interactions
2007
International audience; Molecular pathways governing the life cycle of arbuscular mycorrhizal (AM) fungi and their symbiotic interactions with root tissues are not yet fully understood. Most studies fo fungal responses to host plants have targeted developmental stages before root contact (germinating spores), or after root colonization (intraradical mycelium). We are focusing on the early cell events of appressoria contact with the root surface which are essential to the successful outcome of the AM symbiosis. Recent monitoring of Glomus intraradices gene expression at this stage has revealed differential fungal responses to roots of host and non-host (Myc- mutants) M. truncatula (Seddas et…
Single Cell analysis evidence heterogeneous expression of the Agr communication system of[i] Listeria monocytogenes[/i]
2011
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Highlighting the role of diversity in driving weed dynamics and weed-crop interactions
2017
SPEBIOME INRA; Le Microscope à Dissection Laser (MDL) est composé de l’association d’un microscope équipé d’objectifs permettant un grossissement x2 à x100 et d’un système optique de découpe laser. Il permet de microdisséquer de façon ciblée des échantillons biologiques, de type bactérien, animal ou végétal. Sa finesse de dissection permet de sélectionner, d’isoler puis de récupérer des cellules cibles afin de concentrer le matériel. Ainsi, il est possible de réaliser des analyses de transcriptomique, de génomique ou de protéomique à partir de cellules cibles. Avant de microdisséquer, la préparation des échantillons est primordiale pour s’assurer de récupérer une qualité et une quantité suf…
Differential expression of the agr locus of Listeria monocytogenes EGD-e
2007
International audience