Search results for "Edman degradation"
showing 2 items of 12 documents
Assays of Proteasome-Dependent Cleavage Products
2005
The degradation of misfolded, aged, or no longer needed cytosolic proteins depends largely on the ubiquitin-proteasome system. Proteasomes degrade their substrates into fragments of 3-20 amino acids. Human 20S proteasomes can be purified from human erythrocytes by batch adsorption to DEAE-cellulose, ammonium sulfate precipitation, anion-exchange fast protein liquid chromatography (FPLC), and glycerol density gradient ultracentrifugation. 20S proteasomes purified by this method are suitable for the in vitro digestion of synthetic peptides as well as full-length proteins. The degradation products produced by proteasomes are separated by reversed-phase HPLC using an acetonitrile gradient. The …
Pyrgomorphid grasshoppers of the genus Phymateus contain species-specific decapeptides of the AKH/RPCH family regulating lipid-mobilization during fl…
1996
. Using heterologous and conspecific bioassays, two peptides have been isolated from methanolic extracts of corpora cardiaca from the pyrgomorphid grasshopper Phymateus morbillosus L.The structures of both peptides were elucidated by a combination of Edman degradation, after deblocking the N-terminal pyroglutamic acid residue, and mass spectrometric techniques.One peptide is an octapeptide (pGlu-Leu-Asn-Phe-Ser-Thr-Gly-TrpNH2) which also occurs in other insects and is code-named Scg-AKH-II.The second peptide is a novel decapeptide member of the AKH/RPCH family (pGlu-Leu-Asn-Phe-Thr-Pro-Asn-Trp-Gly-SerNH2 code-named here Phm-AKH.It is the first example of a different peptide in the same genu…