Search results for "Embryo Culture Techniques"

showing 2 items of 32 documents

Soluble ligands and their receptors in human embryo development and implantation.

2014

Extensive evidence suggests that soluble ligands and their receptors mediate human preimplantation embryo development and implantation. Progress in this complex area has been ongoing since the 1980s, with an ever-increasing list of candidates. This article specifically reviews evidence of soluble ligands and their receptors in the human preimplantation stage embryo and female reproductive tract. The focus will be on candidates produced by the human preimplantation embryo and those eliciting developmental responses in vitro, as well as endometrial factors related to implantation and receptivity. Pathways to clinical translation, including innovative diagnostics and other technologies, are al…

medicine.medical_specialtyReproductive Techniques AssistedEndocrinology Diabetes and Metabolismmedicine.medical_treatmentReproductive medicineEmbryonic DevelopmentReceptors Cell SurfaceBiologyEndometriumBioinformaticsLigandsHuman chorionic gonadotropinEmbryo Culture TechniquesEndometriumEndocrinologyPregnancymedicineHumansReceptors Growth FactorBlastocystEmbryo ImplantationReceptors CytokineReceptorGrowth SubstancesFallopian TubesIn vitro fertilisationEmbryoCoculture TechniquesHormonesCulture MediaMicroRNAsmedicine.anatomical_structureBlastocystImmunologyCytokinesFemaleEmbryo qualityEndocrine reviews
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Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequenti…

2008

Objective To compare embryologic and clinical outcomes in terms of preimplantation development, implantation, pregnancy rates, and secretome profile of implanted blastocysts from the preimplantation genetic diagnosis program grown in sequential versus endometrial epithelial cell (EEC) coculture system. Design Retrospective clinical study and prospective experimental study. Setting In vitro fertilization clinical unit and university research laboratory. Intervention(s) Blastomere biopsy, embryo culture, blastocyst transfer, and protein analysis of the media conditioned from implanted embryos obtained from coculture and sequential systems. Main Outcome Measure(s) Clinical study: blastocyst, i…

medicine.medical_specialtymedicine.medical_treatmentProtein Array AnalysisFertilization in VitroBiologyPreimplantation genetic diagnosisEndometriumAndrologyEmbryo Culture TechniquesEndometriumPregnancymedicineSingle Embryo TransferHumansBlastocystEmbryo ImplantationPreimplantation DiagnosisRetrospective StudiesGynecologyIn vitro fertilisationOocyte DonationInterleukin-6Blastocyst TransferObstetrics and GynecologyComputational BiologyEmbryoEmbryo cultureEpithelial CellsCoculture Techniquesmedicine.anatomical_structureBlastocystReproductive MedicineCulture Media Conditionedembryonic structuresGestationFemaleBiomarkersFertility and sterility
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