Search results for "Embryogenesi"

Predicting the Success of Blastocyst Implantation from Morphokinetic Parameters Estimated through CNNs and Sum of Absolute Differences

2019

The process of In Vitro Fertilization deals nowadays with the challenge of selecting viable embryos with the highest probability of success in the implantation. In this topic, we present a computer-vision-based system to analyze the videos related to days of embryo development which automatically extracts morphokinetic features and estimates the success of implantation. A robust algorithm to detect the embryo in the culture image is proposed to avoid artifacts. Then, the ability of Convolutional Neural Networks (CNNs) for predicting the number of cells per frame is novelty combined with the Sum of Absolute Differences (SAD) signal in charge of capturing the amount of intensity changes durin…

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos.

2012

Objective To evaluate correlations between metabolic activity and implantation potential of transferred embryos in a study based on oxygen (O 2 ) consumption (OC) measurements, because O 2 uptake is directly related to the capacity of an embryo to produce energy via adenosine triphosphate. Design Retrospective cohort study. Setting Infertility institute. Patient(s) Five hundred seventy-five injected oocytes in 56 first oocyte donation cycles with embryo transfer on day 3. Intervention(s) None. Main Outcome Measure(s) We analyzed embryo destination viability and implantation depending on the embryo OC rate obtained from 47,741 measurements (up to 85 measurements per embryo, 2–3 measurements …

The expression of an immune-related phenoloxidase gene is modulated inCiona intestinalisovary, test cells, embryos and larva

2015

Two distinct Ciona intestinalis phenoloxidases (CinPO1, 2) had previously been cloned and sequenced. The CinPO2 is involved in innate immunity and is expressed by inflammatory hemocytes that populate the tunic and pharynx vessels as a response to LPS inoculation. In situ hybridization and immunohistochemistry assays on histological section, showed that the expression of this gene and the produced protein are shared with oogenesis, embryogenesis and larval morphogenesis. Intriguingly, upregulation of gene transcription was found in the test cell layer that envelopes the ovary follicle, ovulated egg, and gastrula, as well as it was modulated in the zygotic nucleus of outer balstomers of 32-ce…

Effects of Tributyltin(IV) Chloride Exposure on Larvae ofCiona intestinalis (Urochordata): An Ultrastructural Study

1996

The effects of tributyltin(IV) chloride (TBT chloride) have been tested on embryos of the ascidian Ciona intestinalis, at two different stages of development: (1) before hatching (coiled larval stage) and (2) 2 h after hatching (swimming larval stage). In vivo observations carried out with a light microscope showed that embryos at the coiled larval stage did not hatch following exposure to TBT chloride. Severe anomalies in the swimming larva, mainly concerning the morphology of the tail, which appeared twisted and squatter than in the controls, were observed. Such anomalies were also found at a functional level, i.e. contractile movements were poor so that the larvae appeared motionless. Ul…

Development of multicellular pollen of Eriobotrya japonica Lindl. through anther culture

2006

8 pages, 5 figures.-- Printed version published Dec 2006.

Anatomy of Lower Limb

2022

In order to understand the pathophysiology of vascular edema, one should comprehend the anatomy of the veins and lymphatics that drain the lower limb. In this chapter, the organization of venous and lymphatic systems is described, together with embryological development of the lower extremity veins, which is of particular importance if anatomical variants are associated with limb swelling. Veins of the lower extremity can be categorized into three hierarchically ordered groups: the superficial, interfascial, and deep ones. Superficial veins are defined as veins located above the superficial fascia. Deep veins are located below the muscular fascia. Interfascial veins are enclosed by these tw…

CHARACTERISATION OF MICROSPORE EMBRYOGENESIS IN ANTHER CULTURE OF CITRUS.

2002

Telomeres as a molecular marker of male infertility

2018

In recent years, male infertility has become a growing social problem. Standard diagnostic procedures, based on assessing seminological parameters, are often insufficient to explain the causes of male infertility. Because of this, new markers with better clinical application are being sought. One of the promising markers seems to be an assessment of telomere length of sperm. Sperm telomeres, in contrast to somatic cells, are elongated as men age. The results of some studies suggest that telomere length may be relevant in the case of fertilization and normal embryo development. Literature reports indicate that there is a correlation between telomere length of sperm and abnormal sperm paramet…

Identification and characterization of PlAlix, the Alix homologue from the Mediterranean sea urchin Paracentrotus lividus.

2013

The sea urchin provides a relatively simple and tractable system for analyzing the early stages of embryo development. Here, we use the sea urchin species, Paracentrotus lividus, to investigate the role of Alix in key stages of embryogenesis, namely the egg fertilization and the first cleavage division. Alix is a multifunctional protein involved in different cellular processes including endocytic membrane trafficking, filamentous (F)-actin remodeling, and cytokinesis. Alix homologues have been identified in different metazoans; in these organisms, Alix is involved in oogenesis and in determination/differentiation events during embryo development. Herein, we describe the identification of th…

Sulfate toxicity to early life stages of European whitefish (Coregonus lavaretus) in soft freshwater

2020

Sulfate occurs naturally in the aquatic environment but its elevated levels can be toxic to aquatic life in freshwater environments. We investigated the toxicity of sulfate in humic, soft freshwater to whitefish (Coregonus lavaretus) from fertilization of eggs to hatching i.e. during the critical phases of whitefish early development. Anadromous Kokemäenjoki whitefish eggs and sperm during fertilization, embryos and larvae were exposed in the long-term 175-day incubation to seven different sodium sulfate (Na2SO4) concentrations from 44 to 2 000 mg SO4 L−1. Endpoint variables were the fertilization success, offspring survival and larval growth. Egg fertilization and early embryonic developmen…