Search results for "Embryonic Structures"
showing 10 items of 623 documents
Supplementary Material for: The Impact of Ovarian Stimulation on the Expression of Candidate Reprogramming Genes in Mouse Preimplantation Embryos
2017
Ovarian stimulation with gonadotrophins is an integral part of assisted reproductive technologies in human subfertility/infertility treatment. Recent findings have associated ovarian stimulation with the increased incidence of imprinting disorders in humans as well as defects in genome-wide methylation reprogramming and, in particular, imprinting in mice. Here, we present the first study that determined the impact of ovarian stimulation on the expression of developmentally important reprogramming genes (Apex1, Lig1, Lig3, Mbd2, Mbd3, Mbd4, and Polb) in single early mouse morula embryos (16-cell stage). Using absolute quantification of mRNA by quantitative real-time PCR, we observed an assoc…
Mecanismos moleculares de proliferación y diferenciación durante el desarrollo embrionario del pez medaka (Oryzias latipes)
2010
A lo largo del desarrollo embrionario las células que forman el embrión deben dividirse numerosas veces hasta alcanzar la cantidad celular capaz de asegurar la diferenciación de todos los tejidos y órganos. Por tanto, la correcta regulación de la proliferación y diferenciación celular es fundamental para evitar defectos durante el desarrollo embrionario y la aparición de enfermedades en el individuo adulto. Aprovechando las numerosas ventajas que ofrece el pez Medaka (Oryzias latipes; Ol), hemos estudiado los mecanismos moleculares que regulan la proliferación y diferenciación en los embriones de Medaka en dos momentos distintos del desarrollo, uno temprano durante la segmentación y otro ta…
ESM table S1 from How to escape from insect egg parasitoids: a review of potential factors explaining parasitoid absence across the insecta
2020
Egg parasitoid presence/absence and factors, number of species and % of herbivory
ESM table S2 from How to escape from insect egg parasitoids: a review of potential factors explaining parasitoid absence across the Insecta
2020
Host orders for each egg parasitoid genus and percentages
ESM S1 from How to escape from insect egg parasitoids: a review of potential factors explaining parasitoid absence across the Insecta
2020
Location of host eggs by parasitoids and information on most important egg parasitoid genera, incl. figure S1
Additional file 5: of KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome
2015
Data supplementary to the nocodazole destabilization assay shown in Fig. 7 . a, b Replicate images of DMSO or nocodazole-treated hTERT-RPE1 cells. Cells were transfected with SF-TAP-tagged KIAA0556 (detected with anti-FLAG immunostaining; green) or GFP-KIAA0556 and counterstained with anti-acetylated tubulin (red) and DAPI (blue). Cells with high KIAA0556 expression are characterised by a filamentous staining pattern and spots of accumulated KIAA0556 signal. In non-transfected cells, 10 minute nocodazole treatment resulted in the loss of a stabilised MT network (see especially the high exposure images), as judged by loss of (almost) all cytoplasmic acetylated tubulin staining and/or the abs…
Additional file 2: of The phospholipase DDHD1 as a new target in colorectal cancer therapy
2018
Figure S1. DDHD1 silencing. To evaluate DDHD1 silencing a. Real-time PCR and b. Western blot analysis were performed on SW480, HCT116, HS5 and HUVEC transfected for 48 or 72Â h with scrambled siRNA or DDHD1 siRNA. (TIFF 6629Â kb)
Supplementary Material for: 2021 European Thyroid Association Guidelines for the Management of Iodine-Based Contrast Media-Induced Thyroid Dysfunction
2021
Given the fact that a large number of radiological examinations using iodine-based contrast media (ICM) are performed in everyday practice, clinicians should be aware of potential ICM-induced thyroid dysfunction (TD). ICM can induce hyperthyroidism (Hyper) or hypothyroidism (Hypo) due to supraphysiological concentrations of iodine in the contrast solution. The prevalence of ICM-induced TD varies from 1 to 15%. ICM-induced Hyper is predominantly found in regions with iodine deficiency and in patients with underlying nodular goiter or latent Graves’ disease. Patients at risk for ICM-induced Hypo include those with autoimmune thyroiditis, living in areas with sufficient iodine supply. Most cas…
Topography of somatostatin gene expression relative to molecular progenitor domains during ontogeny of the mouse hypothalamus
2010
The hypothalamus comprises alar, basal, and floor plate developmental compartments. Recent molecular data support a rostrocaudal subdivision into rostral (terminal) and caudal (peduncular) halves. In this context, the distribution of neuronal populations expressing somatostatin (Sst) mRNA was analyzed in the developing mouse hypothalamus, comparing with the expression pattern of the genes Orthopedia (Otp), Distal-less 5 (Dlx5), Sonic Hedgehog (Shh), and Nk2 homeobox 1 (Nkx2.1). At embryonic day 10.5 (E10.5), Sst mRNA was first detectable in the anterobasal nucleus, a Nkx2.1-, Shh-, and Otp-positive basal domain. By E13.5, nascent Sst expression was also related to two additional Otp-positiv…
Inhibition of HSP27 blocks fibrosis development and EMT features by promoting Snail degradation
2013
Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by myofibroblast proliferation. Transition of epithelial/mesothelial cells into myofibroblasts [epithelial-to-mesenchymal transition (EMT)] occurs under the influence of transforming growth factor (TGF)-β1, with Snail being a major transcription factor. We study here the role of the heat-shock protein HSP27 in fibrogenesis and EMT. In vitro, we have up- and down-modulated HSP27 expression in mesothelial and epithelial cell lines and studied the expression of different EMT markers induced by TGF-β1. In vivo, we inhibited HSP27 with the antisense oligonucleotide OGX-427 (in phase II clinical trials as anticancer agent)…