Search results for "Endolysosome"

showing 2 items of 2 documents

Biodegradable pH-Sensitive Poly(ethylene glycol) Nanocarriers for Allergen Encapsulation and Controlled Release

2015

In the last decades, the number of allergic patients has increased dramatically. Allergen-specific immunotherapy (SIT) is the only available cause-oriented therapy so far. SIT reduces the allergic symptoms, but also exhibits some disadvantages; that is, it is a long-lasting procedure and severe side effects like anaphylactic shock can occur. In this work, we introduce a method to encapsulate allergens into nanoparticles to avoid severe side effects during SIT. Degradable nanocarriers combine the advantage of providing a physical barrier between the encapsulated cargo and the biological environment as well as responding to certain local stimuli (like pH) to release their cargo. This work int…

Polymers and PlasticsProton Magnetic Resonance SpectroscopyNanoparticleBioengineeringmacromolecular substancesmedicine.disease_causePolyethylene GlycolsBiomaterialschemistry.chemical_compoundAllergenPolymer chemistryPEG ratioMaterials ChemistrymedicineHumansNanotechnologyDrug CarriersAcetalAllergensHydrogen-Ion ConcentrationEndolysosomeControlled releaseCombinatorial chemistrychemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationChromatography GelNanocarriersEthylene glycolBiomacromolecules
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Improvements in Rational Design Strategies of Inulin Derivative Polycation for siRNA Delivery.

2016

The advances of short interfering RNA (siRNA)-mediated therapy provide a powerful option for the treatment of many diseases, including cancer, by silencing the expression of targeted genes involved in the progression of the pathology. On this regard, a new pH-responsive polycation derived from inulin, Inulin-g-imidazole-g-diethylenetriamine (INU-IMI-DETA), was designed and employed to produce INU-IMI-DETA/siRNA "Inulin COmplex Nanoaggregates" (ICONs). The experimental results showed that INU-IMI-DETA exhibited strong cationic characteristics and high solubility in the pH range 3-5 and self-aggregation triggered by pH increase and physiological salt concentration. INU-IMI-DETA showed as well…

polycationssiRNA deliverySmall interfering RNAPolymers and PlasticsInulinBioengineering02 engineering and technology010402 general chemistry01 natural sciencesBiomaterialschemistry.chemical_compoundDrug Delivery SystemsMaterials ChemistryPolyaminesGene silencingHumansGene SilencingRNA Small Interferingpolycations siRNA delivery inulinRational designInulinBafilomycinRNATransfectionHydrogen-Ion Concentration021001 nanoscience & nanotechnologyEndolysosomePolyelectrolytesEndocytosis0104 chemical scienceschemistryBiochemistrySettore CHIM/09 - Farmaceutico Tecnologico ApplicativoDrug DesignMCF-7 Cellspolycations; siRNA delivery; inulin0210 nano-technologyBiomacromolecules
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