Search results for "Endophyte"

Endophytic Bacteria and Essential Oil from Origanum vulgare ssp. vulgare Share Some VOCs with an Antibacterial Activity

2022

Medicinal aromatic plants’ essential oils (EOs) are mixtures of volatile compounds showing antimicrobial activity, which could be exploited to face the emerging problem of multi-drug resistance. Their chemical composition can depend on the interactions between the plant and its endophytic microbiota, which is known to synthesize volatile organic compounds (VOCs). However, it is still not clear whether those volatile metabolites can contribute to the composition of the aroma profile of plants’ EOs. The aims of this study were to characterize medicinal plant O. vulgare ssp. vulgare bacterial endophyte VOCs, evaluating their ability to antagonize the growth of opportunistic human pathogens bel…

Endophytic Bacteria Associated with Origanum heracleoticum L. (Lamiaceae) Seeds

2022

Seed-associated microbiota are believed to play a crucial role in seed germination, seedling establishment, and plant growth and fitness stimulation, due to the vertical transmission of a core microbiota from seeds to the next generations. It might be hypothesized that medicinal and aromatic plants could use the seeds as vectors to vertically transfer beneficial endophytes, providing plants with metabolic pathways that could influence phytochemicals production. Here, we investigated the localization, the structure and the composition of the bacterial endophytic population that resides in Origanum heracleoticum L. seeds. Endocellular bacteria, surrounded by a wall, were localized close to th…

Genomic Analysis of Endophytic Bacillus-Related Strains Isolated from the Medicinal Plant Origanum vulgare L. Revealed the Presence of Metabolic Path…

2022

Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues, (i.e., the endophytic bacterial microbiome) can influence the synthesis of the aforementioned compounds leads to the necessity of unraveling the mechanisms involved in the determination of this symbiotic relationship. Here, we report the genome sequence of four endophytic bacterial strains isolated from the medicinal plant Origanum vulgare L. and able to antagonize the growth of opportunistic pa…

Polyketide Derivatives from Mangrove Derived Endophytic Fungus Pseudopestalotiopsis theae

2020

Chemical investigation of secondary metabolites from the endophytic fungus Pseudopestalotiopsis theae led to the isolation of eighteen new polyketide derivatives, pestalotheols I&ndash

Apulian olive sapwood endophytes to cope with Xylella fastidiosa: community analysis, antagonists screening, colonization efficiency, and genes expre…

2021

This dissertation comprises a series of studies aimed at exploring the cultivable endophytic communities indigenous to the sapwood of Apulian olive cultivars, identifying potential antagonists against Xylella fastidiosa, pauca ST53, and demonstrating their colonization efficiency and associated effects on olive defence-related genes. This work starts with an extensive analysis of cultivable sapwood endophytes from resistant and susceptible cultivars distributed across phytosanitary zones in Apulia. After multiple isolation of endophyte colonies, the results were translated into quantitative indicators. It was found that the resistant cultivar 'Leccino' showed high stability and diversity of…

Metabolites from Combretum dolichopetalum and its associated endophytic fungus Nigrospora oryzae--Evidence for a metabolic partnership.

2015

Abstract A new altersolanol derivative, 4-dehydroxyaltersolanol A ( 9 ), along with two known sesquiterpenoids, ( S )-7′-hydroxyabscisic acid ( 7 ) and ( S )-abscisic acid ( 8 ) were obtained from the endophytic fungus, Nigrospora oryzae , isolated from leaves of Combretum dolichopetalum . The host plant yielded six known compounds including ellagic acid ( 1 ), 3, 3′, 4-tri-O-methylellagic acid ( 2 ), arjunolic acid ( 3 ), 4′-dihydrophaseic acid ( 4 ), echinulin ( 5 ) and arestrictin B ( 6 ). Close structural similarities with regard to compounds 4 , 7 and 8 were observed between the metabolites from the host plant and those of the endophytic fungus. Furthermore compounds 5 and 6 are relate…

Endophytes from Phragmites australis: their role in phytodepuration

Wastewater treatment is an alarming question and phytodepuration represents an efficient and cost-effective solution. Phragmites australis is generally used for phytodepuration and it excellently removes arsenic, iron and nickel from polluted water. The purification potential of its bacterial endophytic community has never been explored though. G.I.D.A. S.p.a uses P. australis for phytoremediation in a pilot plant, at Calice purifier, which treats the effluent from the Membrane Bioreactor plant. Endophytic bacterial strains were isolated from different P. australis compartments sampled before the activation of the Calice purifier and four additional times during a 18-months period, to analy…

Wooden biochar as a carrier for endophytic isolates

2017

Abstract Plant growth promoting microorganisms (PGPM) serve as a basis for bio-fertilizers. The aim of this study was to evaluate the wood-derived biochar as a potential carrier for PGPM, previously isolated from plants. The selected microbial consortium was compatible with aged biochar obtained from tree branches and processed at temperatures of 600–800 °C. The effect of the consortium or biochar alone and biochar carrier-based bio-formulation on plant growth was estimated in a 14-day greenhouse experiment with barley. The length of shoots and roots increased by 46% and 32% respectively in comparison with control. Synergy of consortium with biochar was observed regarding root length. Conso…

Fungal Assemblages Associated with Roots of Halophytic and Non-halophytic Plant Species Vary Differentially Along a Salinity Gradient

2012

Structure of fungal communities is known to be influenced by host plants and environmental conditions. However, in most cases, the dynamics of these variation patterns are poorly understood. In this work, we compared richness, diversity, and composition between assemblages of endophytic and rhizospheric fungi associated to roots of two plants with different lifestyles: the halophyte Inula crithmoides and the non-halophyte I. viscosa (syn. Dittrichia viscosa L.), along a spatially short salinity gradient. Roots and rhizospheric soil from these plants were collected at three points between a salt marsh and a sand dune, and fungi were isolated and characterized by ITS rDNA sequencing. Isolates…

Lulwoana sp., a dark septate endophyte (DSE) in roots of Posidonia oceanica (L.) Delile seagrass

2014

Posidonia oceanica is the most common, widespread and important monocotyledon seagrass in the Mediterranean Basin, and hosts a large biodiversity of species, including microorganisms with key roles in the marine environment. In this study, we ascertain the presence of a fungal endophyte in the roots of P. oceanica growing on different substrata (rock, sand and matte) in two Sicilian marine meadows. Staining techniques on root fragments and sections, in combination with microscope observations, were used to visualise the fungal presence and determine the percentage of fungal colonisation (FC) in this tissue. In root fragments, statistical analysis of the FC showed a higher mean in roots anch…