Search results for "Enterococcus"

In vitro effectiveness of different endodontic irrigants on the reduction of Enterococcus faecalis in root canals

2010

Objective: To compare the reduction of E. faecalis counts in root canals produced by irrigation with distilled water, hydrogen peroxide, sodium hypochlorite, chlorhexidine, and combinations of solutions, in vitro. Study Design: Study sample included sixty mandibular premolar teeth mounted in dental stone. Root canals were prepared using crown down technique under distilled water irrigation. Specimens were sterilized overnight by ethylene oxide gas. Each canal was completely filled up with the E. faecalis suspension and incubated at 37ºC for 24 hours. Root canals were irrigated with the test solutions. A volume of 5 ml of the tested irrigant was used for each sample with an exposure period o…

Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage fr…

2015

The diversity of lactic acid bacteria (LAB) associated with chicha, a traditional maize-based fermented alcoholicbeverage from Northwestern Argentina, was analyzed using culture-dependent and culture-independent approaches.Samples corresponding to 10 production steps were obtained from two local producers at Maimará(chicha M) and Tumbaya (chicha T). Whereas by culture-dependent approach a few number of species(Lactobacillus plantarum and Weissella viridescens in chicha M, and Enterococcus faecium and Leuconostocmesenteroides in chicha T) were identified, a higher quantitative distribution of taxa was found in both beveragesby pyrosequencing. The relative abundance of OTUs was higher in chic…

Prévalence de pathogènes humains dans les sols français, effet des facteurs pédoclimatiques, biologiques et du mode d'utilisation des sols

2013

Soil contamination by bacterial pathogens can occur through manure, sewage sludge spreading or irrigation using waste water treatment plants effluents. Agricultural soils may act as reservoirs for these pathogens, play a significant role in their dissemination, leading to the potential contamination of food and water resources. Health risk associated with the occurrence of pathogens in environmental matrices has to be thoroughly evaluated. In this context, the objectives of this work were: i) to determine the prevalence of two pathogenic bacterial species (Listeria monocytogenes and Enterococcus faecalis) in a large collection of French soils originated from a systematic soil survey of the …

Role of the microbiota in the defense against infections by Enterococci

2017

Resumen de la Tesis : “Role of the microbiota in the defense against infections by Enterococci” Introducción El tracto gastrointestinal está colonizado por centenares de especies de bacterias comensales. Se ha visto en los últimos años que dichas bacterias presentan un papel crucial en la prevención y resolución de enfermedades infecciosas. La microbiota comensal inhibe a patógenos oportunistas mediante mecanismos (i) de interacción directa, por producción de sustancias inhibitorias o competición por nutrientes (ii) indirectos, por la inducción del sistema inmune. El tratamiento con antibióticos altera la composición de la microbiota intestinal promoviendo la colonización intestinal por pat…

Effect of environmental and nutritional factors on the production of bacteriocin-like inhibitory substances (BLIS) by Enterococcus faecium strains

2008

An investigation of the bacteriocinogenic potential of lactic acid bacteria associated with wheat (triticum durum) kernels and non-conventional flours

2008

One hundred and thirty-seven lactic acid bacteria (LAB), previously isolated from wheat (Triticum durum) grains and non-conventional flour samples, were tested for the production of antibacterial substances. A total of 16 strains (5 Enterococcus faecium, 5 Enterococcus mundtii, 4 Pediococcus pentosaceus, 1 Lactobacillus coryniformis and 1 Lactococcus garvieae) were found to inhibit the growth of Listeria innocua. The antibacterial activities were preliminarily investigated for their general behaviour with proteolytic (proteinase K, protease B and trypsin), amylolytic (α-amylase) and lipolytic (lipase) enzymes, after heat treatment, and exposure to different pHs and ethanol concentrations. B…

A polyphasic approach in order to identify dominant lactic acid bacteria during pasta manufacturing

2010

Using a polyphasic approach, we have isolated and identified, lactic acid bacteria (LAB) in samples directly collected from an artisanal pasta-making manufactory located in Puglia (South Italy). Samples were collected during several steps of pasta processing and LAB were isolated on MRS and M17 plates. Furthermore, strains were grouped in a total of eight species by means of randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) typing and 16S rDNA sequencing. The majority of strains were identified as belonging to Pediococcus pentosaceus and Enterococcus faecium species. The remaining strains were characterized and assigned to Weissella confusa, Pediococcus acidilactici,…

In vitro enterococcus faecalis biofilm formation on five adhesive systems

2011

Objective: To determine the E. faecalis biofilm formation on the surface of five adhesive systems (AS) and its relationship with roughness. Study Design: The formation of E. faecalis biofilms was tested on the surface of four dual-cure AS: AdheSE DC, Clearfil DC Bond, Futurabond DC and Excite DSC and one light-cure antimicrobial AS, Clearfil Protect Bond, after 24 hours of incubation, using the MBEC high-throughput device. Results: E. faecalis biofilms grew on all the adhesives. The least growth of biofilm was on Excite DSC, Clearfil Protect Bond, and the control. Futurabond DC resulted in the greatest roughness and biofilm amount. There was a close relationship between the quantity of biof…

Antibacterial activity of different root canal sealers against Enterococcus faecalis

2017

Background The aim of the present study was to compare in vitro the antimicrobial activity of different root canal sealers against Enterococcus faecalis, prior and subsequent to setting. Material and methods Agar diffusion test (ADT) was used for evaluating the antibacterial activity of non-set sealer while the direct contact test (DCT) was used for after setting. Results ADT: Except for TotalFill BC Sealer all the others sealers tested showed antibacterial activity. BioRoot™RCS, MTA Fillapex and Sealapex Root Canal Sealer showed the lowest antibacterial activity, a significant increase in antibacterial effect for both Pulp Canal Sealer™ and AH plus sealers were found. Significantly higher …

Chemomechanical preparation by hand instrumentation and by Mtwo engine-driven rotary files, an ex vivo study

2011

Objective: To compare the disinfecting efficacy of the sodium hypochlorite irrigation by root canal preparation with stainless steel hand files, taper 0.02 and nickel-titanium Mtwo files with taper 0.04-0.06. Study Design: 40 extracted human teeth were sterilized, and then inoculated with Enterococcus faecalis (ATCC 29212). After 6 day incubation time the root canals were prepared by hand with K-files (n=20) and by engine-driven Mtwo files (VDW, Munich, Germany) (n=20). Irrigation was carried out with 2.5% NaOCl in both cases. Samples were taken and determined in colony forming units (CFU) from the root canals before and after the preparation with instruments #25 and #35. Results: Significa…