Search results for "Envelope"
showing 10 items of 336 documents
Cargo transport through the nuclear pore complex at a glance.
2021
ABSTRACT Bidirectional transport of macromolecules across the nuclear envelope is a hallmark of eukaryotic cells, in which the genetic material is compartmentalized inside the nucleus. The nuclear pore complex (NPC) is the major gateway to the nucleus and it regulates nucleocytoplasmic transport, which is key to processes including transcriptional regulation and cell cycle control. Accordingly, components of the nuclear transport machinery are often found to be dysregulated or hijacked in diseases. In this Cell Science at a Glance article and accompanying poster, we provide an overview of our current understanding of cargo transport through the NPC, from the basic transport signals and mach…
A Bimolecular Multicelular complementation system for the detection of syncytium formation: A new methodology for the identification of entry inhibit…
2019
AbstractFusion of viral and cellular membranes is a key step during the viral life cycle. Enveloped viruses trigger this process by means of specialized viral proteins expressed on their surface, the so called viral fusion proteins. There are multiple assays to analyze the viral entry including those that focus on the cell-cell fusion induced by some viral proteins. These methods often rely on the identification of multinucleated cells (syncytium) as a result of cell membrane fusions. In this manuscript, we describe a novel methodology for the study of cell-cell fusion. Our approach, named Bimolecular Multicelular Complementation (BiMuC), provides an adjustable platform to investigate quali…
Role of lipid rafts in virus infection
2009
Rafts are domains of the plasma membrane, enriched in cholesterol and sphingolipids; they form a platform for signaling proteins and receptors. The lipid rafts are utilized in the replication cycle of numerous viruses. Internalization receptors of many viruses localize to rafts or are recruited there after virus binding. Arrays of signal transduction proteins found in rafts contribute to efficient trafficking and productive infection. Some viruses are dependent on raft domains for the biogenesis of their membranous replication structures. Finally, rafts are often important in virus assembly and budding. Subsequently, raft components in the viral envelope may be vital for the entry to a new…
Two mutations in gB-1 and gD-1 of herpes simplex virus type 1 are involved in the "fusion from without" phenotype in different cell types.
1996
Previous studies have shown that certain strains of herpes simplex viruses type 1 (HSV-1) are able to induce “fusion from without” (FFWO) which means no transcription or translation of the viral genome happens. The main determinants for FFWO in BHK cells are mutations in the C-terminal part of gB-1. But single mutations in this part of the genome are not sufficient to transfer the FFWO phenotype also to Vero cells. Here, we report that FFWO of HSV strains indeed need additional mutations in the N-terminal part of gD in order to produce the FFWO phenotype in BHK and Vero cells. By marker transfer we are able to show that loss of mutations in the N-terminal part of gD influences the ability t…
Archaeal Cell Walls
2001
Archaea represent the third domain of life with the Bacteria and Eukarya. They have developed different types of cell envelopes. Keywords: glutaminylglycan; heteropolysaccharide; methanochondroitin; pseudomurein; S-layer (glyco-)protein; Archaea; cell envelope
Hexapeptides that interfere with HIV-1 fusion peptide activity in liposomes block GP41-mediated membrane fusion
2006
AbstractUpon receptor-mediated activation, the gp41 hydrophobic, conserved fusion peptide inserts into the target membrane and promotes the kind of perturbations required for the progression of the HIV-cell fusion reaction. Using a synthetic combinatorial library we have identified all d-amino acid hexapeptide sequences that inhibited the fusion peptide capacity of perturbing model membranes. Two hexapeptides that effectively inhibited the fusion peptide in these systems were subsequently shown to inhibit cell–cell fusion promoted by gp41 expressed at cell surfaces. These observations might be of importance for understanding the mechanisms underlying fusion peptide activity and suggest new …
Spectral investigation of spin echo emission
1993
Abstract The spectral content of the echo radiation emitted after a two-pulse sequence is measured in a two-level spin system. The spectral profiles exhibit maxima and zeroes of spectral density depending on the exciting sequence parameters. A calculation based on a vectorial model relates the zeroes to those packets that happen to be transparent to the second (refocusing) pulse. Moreover we report on a new spectral narrowing effect which we tentatively ascribe to the instantaneous diffusion.
Über polygermane
1980
Abstract A complete series of 5-membered heterocycles (Ph 2 Ge) 4 X with X = O, S, Se and Te has been prepared and investigated by mass spectra, 13 C NMR spectra and vibrational spectra. The 13 C signals of the phenyl groups in the compounds (Ph 2 Ge) 4 X were compared with the related values in the homocycle (Ph 2 Ge) 5 . Frequency values for the symmetrical and asymmetrical stretching vibrations of the units GeGeGe and GeXGe are given. The crystal structure of the compound (Ph 2 Ge) 4 S has been determined and refined to a R of 0.051. The 5-membered ring Ge 4 S exhibits envelope conformation.
The generalized method of equal areas for the design of sum-of-cisoids simulators for mobile Rayleigh fading channels with arbitrary Doppler spectra
2011
We present in this paper a generalized version of the method of equal areas (MEA) that is well suited for the design of sum-of-cisoids (SOC) simulators for narrowband mobile Rayleigh fading channels characterized by any type of Doppler power spectral densities (DPSDs). Unlike the original MEA, the generalized MEA (GMEA) can be applied to the simulation of fading channels with asymmetrical DPSDs. This is an important feature because the simulation of such channels is of great interest for the laboratory analysis of mobile communication systems under non-isotropic scattering conditions. We show that irrespective of the underlying DPSD, the GMEA results in a very good approximation to the auto…
The Impact of Human Walking on the Time-Frequency Distribution of In-Home Radio Channels
2018
Passive activity recognition of home occupants has become a very hot topic in the area of radio communications, as it enables the development of cutting-edge healthcare monitoring solutions. Thanks to ubiquitous radio waves, such as WiFi signals, at today's homes, one can process radio waves reflected off a person's body for identifying certain mobility patterns. This new approach ignores the need for any wearable sensors. This paper reports a challenging indoor radio channel measurement campaign at 5.9 GHz, which has been conducted to study the impact of walking persons on the temporal and spectral properties of the channel. In particular, the time-frequency distribution of the channel has…