Search results for "Environment and public health"

showing 10 items of 85 documents

The oxygen-responsive transcriptional regulator FNR ofEscherichia coli : the search for signals and reactions

1997

The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The sensory and the regulatory functions reside in separate domains of FNR. The sensory domain contains a Fe-S cluster, which is of the [4Fe-4S]2+ type under anaerobic conditions. It is suggested that oxygen is supplied to the cytoplasmic FNR by diffusion and inactivates FNR by direct interaction. Reactivation under anoxic conditions requires cellular reductants. In vitro, the Fe-S cluster is converted to a [3Fe-4S]+ or a [2Fe…

Iron-Sulfur Proteinsinorganic chemicalsEscherichia coli Proteinschemistry.chemical_elementBiologyNitrate reductasemedicine.disease_causeenvironment and public healthMicrobiologyOxygenMetabolic pathwayBacterial ProteinschemistryBiochemistryCytoplasmRespirationEscherichia coliTranscriptional regulationmedicinebacteriaSignal transductionMolecular BiologyEscherichia coliSignal TransductionTranscription FactorsMolecular Microbiology
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Cajaninstilbene acid (CSA) exerts cytoprotective effects against oxidative stress through the Nrf2-dependent antioxidant pathway.

2013

Cajaninstilbene acid (CSA), an active compound separated from pigeon pea leaves, possesses the highly efficient antioxidant activities. Transcription factor nuclear factor-erythroid 2-related factor 2 (Nrf2) is an important regulator of cellular oxidative stress. This study examined the role of Nrf2 in CSA-mediated antioxidant effects on human hepatocarcinoma (HepG2) cell line. The generation of reactive oxygen species (ROS) upon H2O2 and CSA treatment was lower than that of H2O2 alone. CSA activated Nrf2 as evaluated by Western blotting. A luciferase reporter assay also demonstrated that CSA-activated signaling resulted in the increased transcriptional activity of Nrf2 through binding to t…

MAPK/ERK pathwayAntioxidantNF-E2-Related Factor 2medicine.medical_treatmentBlotting WesternBiologyToxicologymedicine.disease_causeenvironment and public healthAntioxidantsStilbenesmedicineNAD(P)H Dehydrogenase (Quinone)HumansProtein kinase BTranscription factorPI3K/AKT/mTOR pathwaychemistry.chemical_classificationReactive oxygen speciesGeneral MedicineHep G2 Cellsrespiratory systemAntioxidant Response ElementsSalicylatesOxidative StressBiochemistrychemistryCytoprotectionNAD+ kinaseOxidative stressHeme Oxygenase-1Signal TransductionToxicology letters
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Docosahexaenoic acid inhibits cancer cell growth via p27Kip1, CDK2, ERK1/ERK2, and retinoblastoma phosphorylation

2006

Docosahexaenoic acid (DHA), a PUFA of the n-3 family, inhibited the growth of FM3A mouse mammary cancer cells by arresting their progression from the late-G(1) to the S phase of the cell cycle. DHA upregulated p27(Kip1) levels by inhibiting phosphorylation of mitogen-activated protein (MAP) kinases, i.e., ERK1/ERK2. Indeed, inhibition of ERK1/ERK2 phosphorylation by DHA, U0126 [chemical MAPK extracellularly signal-regulated kinase kinase (MEK) inhibitor], and MEK(SA) (cells expressing dominant negative constructs of MEK) resulted in the accumulation of p27(Kip1). MAP kinase (MAPK) inhibition by DHA did not increase p27(Kip1) mRNA levels. Rather, this fatty acid stabilized p27(Kip1) contents…

MAPK/ERK pathwayDocosahexaenoic AcidsMammary Neoplasms AnimalQD415-436fatty acidsenvironment and public healthBiochemistryMiceEndocrinologyCyclin-dependent kinaseCyclin EAnimalsRNA MessengerPhosphorylationCells CulturedCell ProliferationMAPK14biologyKinaseCyclin-dependent kinase 4Cyclin-Dependent Kinase 2Cyclin-dependent kinase 2Retinoblastomafood and beveragesCell BiologyUp-RegulationCell biologyenzymes and coenzymes (carbohydrates)cyclin-dependent kinaseCyclin-dependent kinase complexbiology.proteinPhosphorylationcell cyclelipids (amino acids peptides and proteins)Mitogen-Activated Protein KinasesCyclin-Dependent Kinase Inhibitor p27Journal of Lipid Research
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p42 MAPK phosphorylates 80 kDa MARCKS at Ser-113.

1996

Abstract It is demonstrated here that p42 MAPKinase (p42 MAPK) phosphorylates the M yristoylated A lanine- R ich C - K inase S ubstrate (MARCKS) at Ser-113. In permeabilised Swiss 3T3 cells activation of protein kinase C (PKC) leads to p42 MAPK activation, but only the protein kinase C sites in MARCKS become phosphorylated and not Ser-113. The mitogen platelet-derived growth factor (PDGF) elicits the same response. These results demonstrate that while Ser-113 is a substrate for p42 MAPK in vitro and can be phosphorylated in vivo as shown by Taniguchi et al. [(1994) J. Biol. Chem. 269, 18299–18302], its phosphorylation is not subject to acute regulation by p42 MAPK in Swiss 3T3 cells.

MAPK/ERK pathwayMARCKSmedicine.medical_treatmentMitogen-activated protein kinase kinaseBiochemistryenvironment and public healthSubstrate SpecificityMiceStructural BiologySerinep42MAPKinasePhosphorylationMyristoylated Alanine-Rich C Kinase SubstrateCells CulturedProtein Kinase CMitogen-Activated Protein Kinase 1Platelet-Derived Growth FactorbiologyChemistryIntracellular Signaling Peptides and Proteins3T3 CellsProtein-Tyrosine KinasesCell biologyBiochemistryMitogen-activated protein kinasePhosphorylationTetradecanoylphorbol Acetatebiological phenomena cell phenomena and immunityPlatelet-derived growth factor receptorhormones hormone substitutes and hormone antagonistsendocrine systemRecombinant Fusion ProteinsMolecular Sequence DataBiophysicsGeneticsmedicineAnimalsAmino Acid SequenceMARCKSMolecular BiologyProtein kinase CGrowth factorMembrane ProteinsProteinsCell BiologyPeptide FragmentsEnzyme ActivationMolecular Weightenzymes and coenzymes (carbohydrates)Calcium-Calmodulin-Dependent Protein Kinasesbiology.proteinMutagenesis Site-DirectedMitogensFEBS letters
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Comparison of body mass index (BMI) with the CUN-BAE body adiposity estimator in the prediction of hypertension and type 2 diabetes

2016

BACKGROUND: Obesity is a world-wide epidemic whose prevalence is underestimated by BMI measurements, but CUN-BAE (Clínica Universidad de Navarra - Body Adiposity Estimator) estimates the percentage of body fat (BF) while incorporating information on sex and age, thus giving a better match. Our aim is to compare the BMI and CUN-BAE in determining the population attributable fraction (AFp) for obesity as a cause of chronic diseases. METHODS: We calculated the Pearson correlation coefficient between BMI and CUN-BAE, the Kappa index and the internal validity of the BMI. The risks of arterial hypertension (AHT) and diabetes mellitus (DM) and the AFp for obesity were assessed using both the BMI a…

Male:Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Physical Examination::Anthropometry [Medical Subject Headings]:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]ObesidadType 2 diabetesÍndice de masa corporalCUN-BAEBody Mass Index:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Diabetes mellitus0302 clinical medicineMedicine030212 general & internal medicineBody mass indexAdiposity:Persons::Persons::Age Groups::Adult::Aged [Medical Subject Headings]education.field_of_studyDiabetisAnthropometryDiabetes:Diseases::Pathological Conditions Signs and Symptoms::Signs and Symptoms::Body Weight::Overweight::Obesity [Medical Subject Headings]Middle Aged:Anatomy::Tissues::Connective Tissue::Adipose Tissue [Medical Subject Headings]CausalityAdipose TissueBody fatHypertensionObesitatFemaleHipertensióResearch ArticleAdultmedicine.medical_specialty:Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Anthropometry::Body Weights and Measures::Body Mass Index [Medical Subject Headings]Population:Check Tags::Male [Medical Subject Headings]030209 endocrinology & metabolismBody adiposity index:Health Care::Environment and Public Health::Public Health::Epidemiologic Factors::Causality [Medical Subject Headings]03 medical and health sciences:Diseases::Cardiovascular Diseases::Vascular Diseases::Hypertension [Medical Subject Headings]Population attributable fractionClassification of obesityInternal medicineHipertensiónHumansObesityeducation:Persons::Persons::Age Groups::Adult [Medical Subject Headings]Aged:Phenomena and Processes::Physiological Phenomena::Body Constitution::Body Composition::Body Fat Distribution::Adiposity [Medical Subject Headings]Body volume indexbusiness.industryPublic Health Environmental and Occupational Health:Diseases::Endocrine System Diseases::Diabetes Mellitus::Diabetes Mellitus Type 2 [Medical Subject Headings]:Persons::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]medicine.diseaseObesityCross-Sectional StudiesEndocrinologyDiabetes Mellitus Type 2:Check Tags::Female [Medical Subject Headings]Attributable riskbusinessBody mass indexDemographyAntropometria
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Gut microbiota steroid sexual dimorphism and its impact on gonadal steroids: influences of obesity and menopausal status

2020

[Background]: Gonadal steroid hormones have been suggested as the underlying mechanism responsible for the sexual dimorphism observed in metabolic diseases. Animal studies have also evidenced a causal role of the gut microbiome and metabolic health. However, the role of sexual dimorphism in the gut microbiota and the potential role of the microbiome in influencing sex steroid hormones and shaping sexually dimorphic susceptibility to disease have been largely overlooked. Although there is some evidence of sex-specific differences in the gut microbiota diversity, composition, and functionality, the results are inconsistent. Importantly, most of these studies have not taken into account the go…

Male:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Cross-Sectional Studies [Medical Subject Headings]PhysiologyGut flora:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Mice:Chemicals and Drugs::Hormones Hormone Substitutes and Hormone Antagonists::Hormones::Gonadal Hormones [Medical Subject Headings]0302 clinical medicineOverweight persons:Organisms::Eukaryota::Animals [Medical Subject Headings]TestosteroneProgesteronaGonadal Steroid HormonesTestosteronaTestosteroneProgesterone:Persons::Persons::Age Groups::Adult::Aged [Medical Subject Headings]Sex Characteristics0303 health sciencesMicrobiotaIntestins -- MalaltiesMiddle AgedSex ; Gender ; Gonadal steroids ; Testosterone ; Progesterone ; Microbiome ; Sexual dimorphism.Persones obeses3. Good healthMenopause:Phenomena and Processes::Microbiological Phenomena::Microbiota [Medical Subject Headings]Intestins -- MicrobiologiaCaracteres sexualeslcsh:QR100-130Female:Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Climacteric::Menopause [Medical Subject Headings]SexAnimal studiesIntestines -- DiseasesMenopauseMenopausaAdultMicrobiology (medical):Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Sex Characteristics [Medical Subject Headings]:Check Tags::Male [Medical Subject Headings]SexoSteroid biosynthesisBiologyIntestines -- Microbiologydigestive systemMicrobiologylcsh:Microbial ecology:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Epidemiologic Study Characteristics as Topic::Epidemiologic Studies::Case-Control Studies [Medical Subject Headings]03 medical and health sciencesSexual dimorphismmedicineAnimalsHumansObesityMicrobiome:Persons::Persons::Age Groups::Adult [Medical Subject Headings]Aged030304 developmental biology:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings]ResearchGender:Persons::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings]medicine.diseasebiology.organism_classificationObesityGastrointestinal Microbiome:Diseases::Nutritional and Metabolic Diseases::Nutrition Disorders::Overnutrition::Obesity [Medical Subject Headings]Gonadal steroidsSexual dimorphismCross-Sectional Studies:Check Tags::Female [Medical Subject Headings]Case-Control StudiesIdentidad de géneroMicrobiome030217 neurology & neurosurgeryMicrobiome
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The systemic lupus erythematosus IRF5 risk haplotype is associated with systemic sclerosis.

2013

Systemic sclerosis (SSc) is a fibrotic autoimmune disease in which the genetic component plays an important role. One of the strongest SSc association signals outside the human leukocyte antigen (HLA) region corresponds to interferon (IFN) regulatory factor 5 (IRF5), a major regulator of the type I IFN pathway. In this study we aimed to evaluate whether three different haplotypic blocks within this locus, which have been shown to alter the protein function influencing systemic lupus erythematosus (SLE) susceptibility, are involved in SSc susceptibility and clinical phenotypes. For that purpose, we genotyped one representative single-nucleotide polymorphism (SNP) of each block (rs10488631, r…

MaleLinkage disequilibrium:Phenomena and Processes::Genetic Phenomena::Phenotype [Medical Subject Headings]Polimorfismo de nucleótido simpleSLElcsh:MedicineAutoimmunityGenome-wide association studyLinkage DisequilibriumScleroderma:Phenomena and Processes::Genetic Phenomena::Genotype::Haplotypes [Medical Subject Headings]:Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings]Gene Frequency:Named Groups::Persons::Population Groups::Continental Population Groups::European Continental Ancestry Group [Medical Subject Headings]Risk FactorsIRF5Genetics of the Immune SystemLupus Erythematosus Systemic:Diseases::Skin and Connective Tissue Diseases::Skin Diseases::Scleroderma Systemic [Medical Subject Headings]skin and connective tissue diseaseslcsh:ScienceMultidisciplinary:Diseases::Immune System Diseases::Autoimmune Diseases::Lupus Erythematosus Systemic [Medical Subject Headings]Predisposición genética a la enfermedad:Phenomena and Processes::Genetic Phenomena::Genetic Linkage::Linkage Disequilibrium [Medical Subject Headings]:Phenomena and Processes::Genetic Phenomena::Genotype::Genetic Predisposition to Disease [Medical Subject Headings]PhenotypeInterferon Regulatory FactorsSYSTEMIC SCLEROSISMedicineEvaluation of complex medical interventions Auto-immunity transplantation and immunotherapy [NCEBP 2]FemaleIRF5; SLE; TYPE I INTERFERON; SYSTEMIC SCLEROSISHaplotiposResearch ArticleFactores de riesgoImmunology:Chemicals and Drugs::Amino Acids Peptides and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::Adaptor Proteins Signal Transducing::Interferon Regulatory Factors [Medical Subject Headings]:Check Tags::Male [Medical Subject Headings]:Health Care::Environment and Public Health::Public Health::Epidemiologic Factors::Causality::Risk Factors [Medical Subject Headings]Single-nucleotide polymorphismHuman leukocyte antigenBiologyPolymorphism Single NucleotideWhite PeopleAutoimmune DiseasesRheumatologyLupus eritematoso sistémicoGeneticsHumansGenetic Predisposition to DiseaseGrupo de ascendencia continental europeaAlleleBiologyAllele frequencyAllelesGenetic Association Studies:Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genes::Alleles [Medical Subject Headings]Scleroderma SystemicHaplotypelcsh:R:Phenomena and Processes::Genetic Phenomena::Genetic Structures::Genome::Genome Components::Genetic Loci [Medical Subject Headings]Human Genetics:Phenomena and Processes::Genetic Phenomena::Genetic Variation::Polymorphism Genetic [Medical Subject Headings]Factores reguladores del interferónHaplotypesDesequilibrio de ligamiento:Check Tags::Female [Medical Subject Headings]Genetic LociTYPE I INTERFERONGenetics of DiseaseImmunologyGenetic PolymorphismClinical Immunologylcsh:Q:Phenomena and Processes::Genetic Phenomena::Gene Frequency [Medical Subject Headings]Population GeneticsIRF5PLoS ONE
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The clinical significance of CDK1 expression in oral squamous cell carcinoma

2014

Objectives: To evaluate the clinical significance of cyclin-dependent kinase 1 (CDK 1 ) in 77 oral squamous cell carcinomas (OSCC) using immunohistochemical methods. Study Design: Immunohistochemical expression of CDK 1 was compared with various clinicopathological features in 77 OSCC and 60 controlled epithelia adjacent to the tumours. In addition, correlation of CDK 1 expression and prognostic and the 5-year accumulative survival rate of OSCC were investigated. Results: The CDK 1 protein was expressed in 52 cases of 77 tumor tissues (67.5%), compared with 21 cases of 60 controlled (35.0%). The expression of CDK 1 was significantly correlated with the histological grade of OSCC ( P <0.05).…

MalePathologymedicine.medical_specialtyCellOdontologíaenvironment and public healthCDC2 Protein KinaseHumansMedicineClinical significanceGeneral DentistrySurvival rateMouth neoplasmCyclin-dependent kinase 1Oral Medicine and Pathologybusiness.industryKinaseCell growthResearchMiddle Aged:CIENCIAS MÉDICAS [UNESCO]Ciencias de la saludImmunohistochemistrySurvival Rateenzymes and coenzymes (carbohydrates)stomatognathic diseasesmedicine.anatomical_structureOtorhinolaryngologyUNESCO::CIENCIAS MÉDICASCarcinoma Squamous CellCancer researchImmunohistochemistryFemaleMouth NeoplasmsSurgerybiological phenomena cell phenomena and immunitybusinessMedicina Oral Patología Oral y Cirugia Bucal
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Is hospital discharge administrative data an appropriate source of information for cancer registries purposes? Some insights from four Spanish regist…

2010

Abstract Background The use of hospital discharge administrative data (HDAD) has been recommended for automating, improving, even substituting, population-based cancer registries. The frequency of false positive and false negative cases recommends local validation. Methods The aim of this study was to detect newly diagnosed, false positive and false negative cases of cancer from hospital discharge claims, using four Spanish population-based cancer registries as the gold standard. Prostate cancer was used as a case study. Results A total of 2286 incident cases of prostate cancer registered in 2000 were used for validation. In the most sensitive algorithm (that using five diagnostic codes), e…

MalePathologymedicine.medical_specialtyPediatricsNeoplasias de la próstataEspañaPopulationMEDLINESensitivity and SpecificityHealth administration:Diseases::Neoplasms::Neoplasms by Site::Urogenital Neoplasms::Genital Neoplasms Male::Prostatic Neoplasms [Medical Subject Headings]Prostate cancer:Publication Characteristics::Study Characteristics::Validation Studies [Medical Subject Headings]Research articlemedicineHumansRegistros de hospitalesRegistries:Publication Characteristics::Study Characteristics::Case Reports [Medical Subject Headings]Medical diagnosisDiagnostic Errorseducation:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Data Collection::Registries [Medical Subject Headings]:Geographicals::Geographic Locations::Europe::Spain [Medical Subject Headings]education.field_of_studybusiness.industryHealth Policylcsh:Public aspects of medicineCancerProstatic Neoplasms:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Data Collection::Records as Topic::Medical Records::Medical Record Linkage [Medical Subject Headings]lcsh:RA1-1270Gold standard (test)medicine.diseaseHospital RecordsPatient DischargeEstudios de validaciónSpainPopulation SurveillanceSistema de registrosDiagnosis codeForms and Records ControlMedical Record LinkageRegistro médico oordinado:Health Care::Environment and Public Health::Public Health::Epidemiologic Methods::Data Collection::Records as Topic::Medical Records [Medical Subject Headings]businessAlgorithmsBMC Health Services Research
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Association of a polyuridylate-specific endoribonuclease with small nuclear ribonucleo-proteins which had been isolated by affinity chromatography us…

1983

Immunoglobulins, containing antibodies against U1-snRNP, have been prepared from a patient with systemic lupus erythematosus. After coupling these antibodies to a Sepharose matrix, U-snRNPs have been isolated and purified from rat liver nuclei by use of immunoaffinity chromatography. The resulting RNPs had the typical protein pattern of U-sn RNPs and a sedimentation coefficient of 12 S. The U-snRNP preparation was associated with an endoribonuclease which required Mg2+ for optimal activity. The enzyme, with an pH optimum of 6.2, degraded only poly(U). Other single-stranded polyribo- and polydeoxyribonucleotides, tRNA, as well as double-stranded RNA and DNA were not digested. The products of…

MalePoly UEndoribonucleaseAntibody AffinityBiologyenvironment and public healthBiochemistryChromatography AffinitySubstrate SpecificitySepharosechemistry.chemical_compoundAffinity chromatographyEndoribonucleasesAnimalsHumansLupus Erythematosus Systemicchemistry.chemical_classificationImmunochemistryRNARats Inbred StrainsRibonucleoproteins Small NuclearMolecular biologyRatsEnzymechemistryLiverRibonucleoproteinsAntibodies AntinuclearImmunoglobulin GRNA splicingTransfer RNADNAEuropean journal of biochemistry
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