Search results for "Enzyme-linked immunoassays"

showing 3 items of 13 documents

A population-based screening for hepatitis C antibodies and active infection using a point-of-care test in a low prevalence area

2020

Background Data on the true prevalence of hepatitis C virus (HCV) infection in the general population is essential. We evaluated a program implementing free universal HCV screening using a non-invasive point-of-care test (POCT) (OraQuick-HCV rapid test) in oral fluid in an urban area in Valencia, South-Eastern Spain. Methods A cross-sectional study was performed during 2015–2017. Free HCV screening was offered by regular mail to 11,500 individuals aged 18 and over, randomly selected from all census residents in the Health Department. All responding participants filled in a questionnaire about HCV infection risk factors and were tested in their tertiary Hospital. In those with a positive POC…

MaleRNA virusesSustained Virologic ResponseUrban PopulationEpidemiologyPhysiologyCross-sectional studyHepacivirusmedicine.disease_causeBiochemistryGeographical locations0302 clinical medicineRisk FactorsImmune PhysiologyPrevalence030212 general & internal medicineEnzyme-Linked ImmunoassaysYoung adultPathology and laboratory medicineAged 80 and overResponse rate (survey)education.field_of_studyImmune System ProteinsMultidisciplinaryHepatitis C virusQRMiddle AgedMedical microbiologyHepatitis CEuropeVirusesRNA ViralMedicineFemale030211 gastroenterology & hepatologyStandardized ratePathogensResearch ArticleAdultmedicine.medical_specialtyAdolescentPoint-of-Care SystemsScienceHepatitis C virusPoint-of-care testingImmunologyPopulationResearch and Analysis MethodsAntiviral AgentsMicrobiologyAntibodiesYoung Adult03 medical and health sciencesInternal medicinemedicineHumansEuropean UnionRisk factorImmunoassayseducationAgedMedicine and health sciencesBiology and life sciencesFlavivirusesbusiness.industryOrganismsViral pathogensProteinsHepatitis C AntibodiesFibrosisHepatitis virusesMicrobial pathogensHealth CareCross-Sectional StudiesSpainMedical Risk FactorsImmunologic TechniquesInterferonsPeople and placesbusinessDevelopmental BiologyPLOS ONE
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Home-based HIV testing: Using different strategies among transgender women in Argentina.

2019

Background In Argentina, HIV prevalence among transgender women (TGW) has been reported at 34%. The stigma is one of the most important factors limiting their access to healthcare services. The aims of this study were to compare different HIV testing methodologies, to determine the factors associated with HIV diagnosis and to determine the feasibility of a home-based HIV testing service for TGW. Methods A multidisciplinary team performed home-based HIV testing interventions in four cities of Argentina. Participants self-identified as TGW, older than 14 years and with a negative or unknown HIV status. Blood samples were screened by two rapid tests (RT), one based on antibodies (Determine™ HI…

RNA virusesMultivariate analysisEpidemiologyPsychological interventionSocial SciencesHIV InfectionsLogistic regressionPathology and Laboratory MedicineTransgender womenGeographical locations0302 clinical medicineImmunodeficiency VirusesSociologyMedicine and Health Sciences030212 general & internal medicineYoung adultEnzyme-Linked Immunoassayseducation.field_of_studyMultidisciplinarySchoolsQRvirus diseasesHIV diagnosis and managementMedical MicrobiologyHIV epidemiologyViral PathogensVirusesMedicineInfectious diseasesFemalePathogens0305 other medical scienceViral loadResearch ArticleAdultmedicine.medical_specialtySciencePopulationArgentinaSexually Transmitted DiseasesHiv testingViral diseasesResearch and Analysis MethodsTransgender PersonsMicrobiologyEducation03 medical and health sciencesYoung AdultInternal medicineRetrovirusesmedicineHumanseducationImmunoassaysMicrobial Pathogens030505 public healthbusiness.industryLentivirusOrganismsBiology and Life SciencesHIVSouth AmericaDiagnostic medicineLogistic ModelsMultivariate AnalysisImmunologic TechniquesFeasibility StudiesReagent Kits DiagnosticPeople and placesbusinessPloS one
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Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy.

2021

Visceral leishmaniasis (VL) caused by Leishmania infantum is endemic in the Mediterranean basin with most of the infected human patients remaining asymptomatic. Recently, the saurian-associated Leishmania tarentolae was detected in human blood donors and in sheltered dogs. The circulation of L. infantum and L. tarentolae was investigated in humans, dogs and cats living in the Pelagie islands (Sicily, Italy) by multiple serological and molecular testing. Human serum samples (n = 346) were tested to assess the exposure to L. infantum by immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) and to L. tarentolae by IFAT. Meanwhile, sera from do…

dogsreal-time polymerase chain reactionRC955-962Pathology and Laboratory MedicineSerologyMedical ConditionswesternZoonosesArctic medicine. Tropical medicineitalymiddle agedMedicine and Health Sciences80 and overadult; aged; aged 80 and over; animals; blotting western; cat diseases; cats; dog diseases; dogs; enzyme-linked immunosorbent assay; female; humans; italy; leishmaniasis visceral; male; middle aged; prevalence; public health; real-time polymerase chain reaction; serologic tests; sicily; surveys and questionnaires; young adult; leishmania infantumEnzyme-Linked ImmunoassayshumansAged 80 and overProtozoansLeishmaniaMammalsCATSmedicine.diagnostic_testbiologyadultpublic healthEukaryotablottingvisceralanimalsagedInfectious DiseasesSerologyfemaleVertebratesleishmania infantumLeishmaniasis Visceralyoung adultLeishmania infantumAntibodyDog DiseasePublic aspects of medicineRA1-1270dog diseasesHumanResearch ArticleNeglected Tropical DiseasesBlotting Westernprevalenceserologic testsImmunofluorescenceResearch and Analysis MethodsmaleSurveys and Questionnaires ...parasitic diseasesmedicineParasitic DiseasessicilyImmunoassaysleishmaniasisProtozoan InfectionsAnimalcatsPublic Health Environmental and Occupational HealthOrganismsBiology and Life SciencesLeishmaniasiscat diseasesbiology.organism_classificationmedicine.diseaseLeishmaniaCat DiseaseTropical DiseasesVirologyParasitic ProtozoansVisceral leishmaniasisAmniotessurveys and questionnairesbiology.proteinImmunologic Techniquesenzyme-linked immunosorbent assayZoologyPLoS Neglected Tropical Diseases
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