Search results for "Erich"

A single mutation in the recombinant light chain of tetanus toxin abolishes its proteolytic activity and removes the toxicity seen after reconstituti…

1994

Specific proteolysis by the tetanus toxin light chain of a vesicle-associated membrane protein (VAMP) involved in exocytosis is thought to underlie its intracellular blockade of neurotransmitter release. To substantiate this mechanism, recombinant light chain was expressed as a maltose binding protein-light chain fusion product in Escherichia coli. After purification of affinity chromatography and cleavage with factor Xa, the resultant light chain was isolated and its identity confirmed by Western blotting and N-terminal sequencing. It exhibited activity similar to that of the native light chain in proteolyzing its target in isolated bovine small synaptic vesicles and in hydrolyzing a 62-re…

Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase.

2001

Abstract Pentachlorophenol 4-monooxygenase (PCP4MO) from Sphingomonas chlorophenolica is a flavoprotein that hydroxylates PCP in the presence of NADPH and oxygen. In order to investigate the structure and function of active site, recombinant PCP4MO (rePCP4MO) was produced in Escherichia coli as a glutathione S-transferase (GST) fusion protein. Moreover, a tobacco etch virus (TEV) protease cleavage site (EKLYFQG) was introduced into GST-PCP4MO and a his-tagged TEV protease was employed. Hence, a two-step purification protocol was developed which allowed obtaining 15–20 mg of rePCP4MO from 1 L culture. The rePCP4MO revealed identity with native enzyme by SDS–PAGE and N-terminal sequence analy…

IL SOFTWARE IRRILAB PER GLI IMPIANTI MICROIRRIGUI: VERIFICHE NUMERICHE E SPERIMENTALI

2018

Nell’ambito delle attività di ricerca condotte presso il Dipartimento di Scienze Agrarie, Alimentari e Forestali dell’Università di Palermo è stato di recente messo a punto un nuovo metodo di calcolo ottimale delle ali gocciolanti, usate nella mi-croirrigazione, attualmente patent pending (“Metodo speditivo per il dimensiona-mento di ali gocciolanti in pendenza”, Università deli Studi di Palermo, Ministero dello Sviluppo Economico - Ufficio Italiano Brevetti e Marchi). Il metodo è stato successivamente esteso ai settori microirrigui, di forma rettangolare e comunque inclinati, in un software applicativo, denominato IrriLab. L’obiettivo del lavoro è quello di “testare” il funzionamento del s…

New Modalities in Knee Osteoarthritis Treatment Using Autologous Bone Marrow-Derived Mononuclear Cells

2018

Abstract The clinical effects on knee osteoarthritis (OA) symptoms and tissue structure were evaluated after bone marrow-derived mononuclear cell intraarticular injection. A group of 32 patients with 34 knee joints in stage II–III osteoarthritis were treated by intraarticular injection of mononuclear cell suspension. Clinical results were obtained by KOOS (Knee Osteoarthritis Outcome Score) and KSS (Knee Society Score) scores during a 12 months follow-up period. Radiological evaluation was performed using magnetic resonance imaging. A comparison with a control group of 28 patients treated with routinely used three hyaluronic acid intra-articular injections was made. No adverse effects were …

Comportamento reologico di nanocompositi a base di miscele HDPE/PA6

2012

La preparazione di sistemi nanocompositi a base di miscele polimeriche suscita notevole interesse perché offre potenzialmente l’opportunità di combinare in maniera sinergica in un unico materiale diverse proprietà non possedute singolarmente da nessun polimero. In generale, le caratteristiche di questi sistemi polifasici dipendono certamente dalla natura dei costituenti, ma anche dalla morfologia finale del materiale: il controllo della dispersione e della dimensione delle particelle nonché dell’adesione interfacciale tra fasi (polimero/polimero, polimero/carica) costituiscono perciò parametri critici per il successo di questi materiali. Scopo di questo lavoro è stato valutare l’influenza d…

Laughing at Oneself : on the New Social Character

2015

Le Prostatiti batteriche. Inquadramento nosologico e indicazioni diagnostico-terapeutiche.

2008

Erich Neumann : Origines et histoire de la conscience (Traduction)

2015

International audience

Erich Neumann: Jacob et Esaü. L'archétype des frères ennemis, un symbole du judaïsme (Traduction)

2016

International audience

The state-of-the-art of collaborative technologies for initial vocational education : a systematic literature review

2018


 
 
 Future workplaces require collaboration skills in which members of different work communities use technologies to solve complex problems. Vocational education and training (VET) programs need to meet the challenge to prepare students to be part of a competent workforce. Particularly initial vocational education is under pressure to develop learners’ collaboration skills and abilities. To date, however, no attempt has been made to perform a comprehensive review of the use of computer-supported collaborative learning (CSCL) technologies across different vocational education settings to account for contextual factors of VET. In this systematic review, 26 published studies …