Search results for "Ethano"
showing 10 items of 2216 documents
To Swim or Not to Swim: Potential Transmission of Balaenophilus manatorum (Copepoda: Harpacticoida) in Marine Turtles
2017
Species of Balaenophilus are the only harpacticoid copepods that exhibit a widespread, obligate association with vertebrates, i.e., B. unisetus with whales and B. manatorum with marine turtles and manatees. In the western Mediterranean, juveniles of the loggerhead sea turtle, Caretta caretta are the only available hosts for B. manatorum, which has been found occurring at high prevalence (>80%) on them. A key question is how these epibionts are transmitted from host to host. We investigated this issue based on experiments with live specimens of B. manatorum that were cultured with turtle skin. Specimens were obtained from head-started hatchlings of C. caretta from the western Mediterranean. …
Pulsed electric field assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organi…
2015
Abstract This work studies the potential of the pulsed electric field (PEF) assisted extraction of nutritionally valuable compounds from microalgae Nannochloropsis spp. using the binary mixture of organic solvents (dimethyl sulfoxide, DMSO and ethanol, EtOH) and water. The one-stage (E I ) and two stage (E II ) extraction procedures were compared. The procedure E I included the common extraction using the binary mixtures. Two stage (E II ) extraction procedure involved PEF-treatment (20 kV/cm) of microalgae suspension (1% wt.) and extraction in water as the first step. The second step included the common extraction using the binary mixtures. The effects of extraction procedure and concentra…
Co-digestion of harvested microalgae and primary sludge in a mesophilic anaerobic membrane bioreactor (AnMBR): Methane potential and microbial divers…
2020
Abstract Anaerobic co-digestion of primary sludge and raw microalgae (Scenedesmus and Chlorella) was performed in a lab-scale semi-continuous anaerobic membrane bioreactor to assess the biological performance and identify the microbial community involved in the co-digestion process. The reactor was operated at 35 °C for 440 days, working at a solids retention time of 100 days. The system achieved 73% biodegradability and showed high stability in terms of pH and volatile fatty acids. An enriched microbial community was observed. Of the several phyla, Chloroflexi and Proteobacteria were the most abundant. Cellulose-degraders phyla (Bacteroidetes, Chloroflexi and Thermotogae) were detected. Sy…
Anaerobic degradation of 2-propanol: Laboratory and pilot-scale studies
2017
Abstract The anaerobic degradation of 2-propanol, an important industrial solvent, was scaled-up from batch assays to a pilot expanded granular sludge bed (EGSB) reactor at 25 °C. Batch studies indicated that 2-propanol followed Haldane kinetics, with a maximum rate at 10 g COD L −1 . Concentrations as high as 25 g COD L −1 did not inhibit the degradation of ethanol, a common co-solvent. Similar specific methanogenic activities (SMA) were obtained for water-solvent and water-brewery sludges (88 and 77 ml CH 4 g-VS −1 d −1 at 5 g COD L −1 ). Continuous degradation showed a lag-phase of three weeks with water-brewery sludge. Increases in 2-propanol load from 0.05 to 0.18 kg COD kg-VS −1 d …
Changes in lipid composition in tobacco cells treated with cryptogein , an elicitor from Phytophthora cryptogea
1995
Abstract Changes in lipid composition occurred when tobacco cells (Nicotiana tabacum var. Xanthi) were treated with cryptogein, a proteinaceous elicitor from Phytophthora cryptogea. The most striking change was an increase in acylated steryl glycosides and steryl esters levels, certainly resulting from the glycosylation and/or esterification of free sterols. Moreover, in vivo pulse-labelling experiments with [14C]acetate also showed that a progressive decline in the incorporation rate of [14C]acetate into free sterols started with the induction of sesquiterpenoid synthesis and lasted when sesquiterpenoid synthesis stops. This phenomenon was accompanied by a significant increase in the synth…
Interspecies interactions mediated by conductive minerals in the sediments of the iron rich meromictic Lake La Cruz, Spain
2019
espanolLa laguna de La Cruz puede considerarse como un analogo biogeoquimico de los ambientes marinos en la Tierra primitiva, porque su columna de agua, muy escasa en sulfato pero rica en metano y hierro, presenta condiciones similares a estos ambientes en el Precambrico. En este ambiente encontramos que particulas conductivas establecen un acoplamiento metabolico entre clados microbianos electroactivos. Demostraremos que el sedimento anoxico de la laguna de La Cruz, rico en minerales de hierro biogeoquimicamente “reactivos”, alberga conocidas especies electroactivas tales como Geobacter y Methanothrix, ademas de otros grupos microbianos que no han sido previamente asociados con un estilo d…
Use of Kluyveromyces marxianus to Increase Free Monoterpenes and Aliphatic Esters in White Wines
2021
An increasing interest in novel wine productions is focused on non-Saccharomyces yeasts due to their potential in improving sensory profiles. Although Kluyveromyces marxianus has been originally isolated from grapes and its enzymatic activities are used in oenology, rarely it has been used as co-starter. The K. marxianus Km L2009 strain has been characterized here and selected as a co-starter both at laboratory- and winery-scale fermentation. The Km L2009 strain showed growth of up to 40 (mg/L) of sulfites and 6% (v/v) of ethanol. Gas chromatographic analysis demonstrates that wines produced by mixed fermentation contain remarkably higher quantities of free monoterpenes and aliphatic esters…
Synthesis and properties of a photoaffinity labeling reagent for protoporphyrinogen oxidases, the target enzymes of diphenyl ether herbicides
1994
A diazoketone 3 has been synthesized in two steps from acifluorfen 1, a diphenyl ether herbicide. Like the parent compound 1, the diazoketone 3 is toxic to plant cells and inhibits protoporphyrinogen oxidase, the molecular target of diphenyl ether herbicides. On photolysis of 3 in methanol, the generated carbene mainly undergoes the Wolff rearrangement to a ketene which further adds methanol, but many other products are observed. A tritiated derivative of 3 has been prepared which is suitable for photoaffinity labeling experiments.
Acclimatised rumen culture for raw microalgae conversion into biogas: Linking microbial community structure and operational parameters in anaerobic m…
2019
[EN] Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spirochaetaceae, Lentimicrobiaceae and Cloacimonetes fermentative and hydrolytic members. During the highest biodegradability achieved in the AnMBR (62%) the dominant microorganisms were Fervidobacterium and Methanosaeta. Different microbial community clusters were observed at different SRT conditions. Interestingly, syntrophic bacteria Gelria and …
Carbon metabolic rates and GHG emissions in different wetland types of the Ebro Delta
2020
Deltaic wetlands are highly productive ecosystems, which characteristically can act as C-sinks. However, they are among the most threatened ecosystems, being very vulnerable to global change, and require special attention towards its conservation. Knowing their climate change mitigating potential, conservation measures should also be oriented with a climatic approach, to strengthen their regulatory services. In this work we studied the carbon biogeochemistry and the specific relevance of certain microbial guilds on carbon metabolisms of the three main types of deltaic wetlands located in the Ebro Delta, north-eastern Spain, as well as how they deal with human pressures and climate change ef…