Search results for "Europa."

Genetic similarity among Tunisian cultivated olive estimated through SSR markers

2013

Olive (Olea europaea L. subsp. europaea var. europaea) is one of the oldest fruit tree in the Mediterranean basin, and is cultivated for oil and canned fruit. Part of this interest is driven by the economic importance of olive oil which is increasing throughout the world due to its beneficial effect to human health. In Tunisia, olive has great socio-economic importance, with more than 60 millions olive trees cultivated for olive oil production including a wide range of cultivars which are widely extended from the north to the south regions of the country for its high economic value. Here, we applied microsatellites (SSRs) molecular markers to assess the genetic variability of the most impor…

Fatal neuroinvasion and SARS-CoV-2 tropism in K18-hACE2 mice is partially independent on hACE2 expression

2022

ABSTRACTAnimal models recapitulating distinctive features of severe COVID-19 are critical to enhance our understanding of SARS-CoV-2 pathogenesis. Transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) under the cytokeratin 18 promoter (K18-hACE2) represent a lethal model of SARS-CoV-2 infection. The precise mechanisms of lethality in this mouse model remain unclear. Here, we evaluated the spatiotemporal dynamics of SARS-CoV-2 infection for up to 14 days post-infection. Despite infection and moderate pneumonia, rapid clinical decline or death of mice was invariably associated with viral neuroinvasion and direct neuronal injury (including brain and spinal neurons). Neuroinv…

Dejerine-Sottas neuropathy associated with De Novo S79P mutation of the peripheral myelin protein 22 (PMP22) gene

1998

Genetic diversity and clonal variation within the main Sicilian olive cultivars based on morphological traits and microsatellite markers

2014

The richness of Olea europaea (L.) genetic resources in Sicily is well documented. In the last 30 years, mostof the local cultivars, landraces and ecotypes have been gathered together in a large ex-situ collection,containing more than 300 genotypes. In this study, 45 putative clones of the main Sicilian olive cultivarswere characterized morphologically using microsatellite markers to unambiguously identify possiblesuperior genotypes. The microsatellites employed were polymorphic (observed heterozygosity = 0.71;polymorphic information content = 0.59), discriminated 52% of the genotypes and enabled the detectionof intra-cultivar polymorphism, derived from both somatic mutations, indicating th…

Genetics of the Charcot-Marie-Tooth disease in the Spanish Gypsy population: the hereditary motor and sensory neuropathy-Russe in depth

2012

Four private mutations responsible for three forms demyelinating of Charcot-Marie-Tooth (CMT) or hereditary motor and sensory neuropathy (HMSN) have been associated with the Gypsy population: the NDRG1 p.R148X in CMT type 4D (CMT4D/HMSN-Lom); p.C737_P738delinsX and p.R1109X mutations in the SH3TC2 gene (CMT4C); and a G>C change in a novel alternative untranslated exon in the HK1 gene causative of CMT4G (CMT4G/HMSN-Russe). Here we address the findings of a genetic study of 29 Gypsy Spanish families with autosomal recessive demyelinating CMT. The most frequent form is CMT4C (57.14%), followed by HMSN-Russe (25%) and HMSN-Lom (17.86%). The relevant frequency of HMSN-Russe has allowed us to inv…

Indication of a common origin of German and American Families with Familial Amyloidneuropathy Typ II

1999

Abstract The classification of familial amyloid neuropathies (FAP) is traditionally based on clinical and regional aspects. In the last 10 years more than 40 mutations of the transthyretin gene have been found to be responsible for different clinical forms of amyloidosis including familial FAP.FAP II is caused by a mutation on the codon 58 of the transthyretin gene. Only two american kindreds (the Maryland/German and the Ohio family) have previously been reported with FAP II starting in the 3rd or 4th decade by sensory disturbances of the hands typically as a carpal tunnel syndrome. We report on a german family with FAP II from the rhine river area south of Mainz. Four members with typical …

SSR-based DNA fingerprints reveal the genetic diversity of Sicilian olive (Olea europaeaL.) germplasm

2005

Twelve published simple sequence repeat (SSR; microsatellite) markers, belonging to the ssrOeUA-DCA, GAPU and UDO series, were tested in a panel of 46 accessions of olive germplasm belonging to 30 unique cultivars collected in seven Provinces of Sicily. Four well-known reference olive cultivars were also added. The analysis was carried out on an automatic capillary sequencer using fluorescent dyes, and fragment sizes were determined using internal standards. The results allowed us to rank the SSRs assayed according to their information content and reproducibility. Up to 115 alleles were identified (119, if those unique to sport mutations were included), the frequency of which allowed geneti…

Centennial olive trees as a reservoir of genetic diversity

2011

†Background and Aims Genetic characterization and phylogenetic analysis of the oldest trees could be a powerful tool both for germplasm collection and for understanding the earliest origins of clonally propagated fruit crops. The olive tree (Olea europaea L.) is a suitable model to study the origin of cultivars due to its long lifespan, resulting in the existence of both centennial and millennial trees across the Mediterranean Basin. †Methods The genetic identity and diversity as well as the phylogenetic relationships among the oldest wild and cultivated olives of southern Spain were evaluated by analysing simple sequence repeat markers. Samples from both the canopy and the roots of each tr…

Is quality of life different between diabetic and non-diabetic people? The importance of cardiovascular risks.

2017

BackgroundTo analyse and compare the impact of cardiovascular risk factors and disease on health-related quality of life (HRQoL) in people with and without diabetes living in the community.MethodsWe used data of 1,905 people with diabetes and 19,031 people without diabetes from the last Spanish National Health Survey (years 2011 +/- 2012). The HRQoL instrument used was the EuroQol 5D-5L, based on time trade-off scores. Matching methods were used to assess any differences in the HRQoL in people with and without diabetes with the same characteristics (age, gender, education level, and healthy lifestyle), according to cardiovascular risk factors and diseases. Disparities were also analysed for…

P2‐289: PREVALENCE AND CORRELATES OF SUBJECTIVE MEMORY IMPAIRMENT IN A RURAL ITALIAN POPULATION OF SUBJECTS AGED 50 YEARS AND OVER: DATA FROM THE ZAB…

2014

Background: Neuropathology related to dementia slowly accumulates over decades. Consequently, identifying persons at a higher risk of dementia could postpone or prevent dementia by timely targeting modifiable risk factors. In this light, mild cognitive impairment (MCI) has been identified as the transitional stage between normal aging and dementia. So far, the understanding of MCI in thegeneral population remains limited. Therefore,we investigated determinants, MRI-correlates, and prognosis of MCI within the populationbasedRotterdamStudy.Methods:A total of 4198 participants were included in this study. Firstly, we studied age, APOE4 carriership, waist circumference, hypertension, diabetes m…