Search results for "Exon"

showing 10 items of 437 documents

Compromised nuclear envelope integrity drives TREX1-dependent DNA damage and tumor cell invasion

2021

Although mutations leading to a compromised nuclear envelope cause diseases such as muscular dystrophies or accelerated aging, the consequences of mechanically induced nuclear envelope ruptures are less known. Here, we show that nuclear envelope ruptures induce DNA damage that promotes senescence in non-transformed cells and induces an invasive phenotype in human breast cancer cells. We find that the endoplasmic reticulum (ER)-associated exonuclease TREX1 translocates into the nucleus after nuclear envelope rupture and is required to induce DNA damage. Inside the mammary duct, cellular crowding leads to nuclear envelope ruptures that generate TREX1-dependent DNA damage, thereby driving the …

SenescenceExonucleaseDNA damageNuclear Envelope[SDV]Life Sciences [q-bio]Breast NeoplasmsBiologySettore MED/08 - Anatomia PatologicaGeneral Biochemistry Genetics and Molecular BiologyCell LineMicemedicineSettore MED/05 - Patologia ClinicaAnimalsHumansNeoplasm InvasivenessEpithelial–mesenchymal transitionCellular SenescenceEndoplasmic reticulumPhosphoproteinsXenograft Model Antitumor AssaysCell biology[SDV] Life Sciences [q-bio]medicine.anatomical_structureExodeoxyribonucleasesCancer cellProteolysisbiology.proteinTREX1 nuclear envelope rupture DNA damage mammary duct carcinoma tumor invasion senescence breast cancer cGAS confinement epithelial to mesenchymal transition Animals Breast Neoplasms Cell Line Cellular Senescence Collagen Disease Progression Exodeoxyribonucleases Female Humans Mice Neoplasm InvasivenessNuclear Envelope PhosphoproteinsProteolysis Xenograft Model Antitumor Assays DNA DamageDisease ProgressionFemaleCollagenNucleusExtracellular Matrix DegradationDNA Damage
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Sequestration of (CH3)Hg+ by amino‐polycarboxylic chelating agents

2012

Abstract The speciation of mono-methylmercury(II) cation (MeHg+) in the presence of Nitrilotriacetate (NTA), Ethylenediamine-N,N,N′,N′-tetraacetate (EDTA), diethylenetriamine-N,N,N′,N″,N″-pentaacetate (DTPA) and (S,S)- Ethylenediamine-N,N′-disuccinic acid (S,S-EDDS) was investigated at I = 0.1 mol L− 1 (NaCl) with the aim to assess a trend of sequestering capacity of the amino‐polycarboxylic (APCs) ligand class towards this cation in aqueous solution. The results obtained gave evidence for the formation of a mononuclear [MeHg(APC)] complex species, differently protonated MeHg(HiL) species (i = 1 to 3, depending on the APC considered), a mixed hydroxo species [MeHg(APC)(OH)] and a binuclear …

Sequestering abilitymedia_common.quotation_subjectInorganic chemistryMethylmercury(II) Complexones Equilibrium analysis Sequestering ability Speciation studiesProtonationMedicinal chemistryComplexoneMaterials ChemistryChelationSettore CHIM/01 - Chimica AnaliticaPhysical and Theoretical ChemistrySpeciation studiesSpectroscopymedia_commonContaminated soilsAqueous solutionChemistryLigandEquilibrium analysisSequestering AgentCondensed Matter PhysicsComplexonesAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsSpeciationMethylmercury(II)Ionic strengthEquilibrium analysi
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Lipid and apoprotein composition of HDL in partial or complete CETP deficiency

2012

Hyperalphalipoproteinemia, as observed in patients who are either homozygous or heterozygous for cholesteryl ester transfer protein (CETP) deficiency, has been shown to be associated with striking changes in apolipoprotein size distribution, namely, of high-density lipoprotein (HDL) and HDL-like particles. We compared the effect of varying degrees of CETP activity on the HDL apolipoprotein profile in Caucasian CETP-deficient subjects and following pharmacological decrease in CETP activity, using Size Exclusion Chromatography followed by Reverse Phase Protein Array (SEC RPA). The main HDL-associated apolipoproteins (Apo), i.e. ApoA-I, ApoA-II, ApoC-I, and ApoC-III, co-eluted with the HDL pea…

Settore MED/09 - Medicina InternaApolipoprotein BCholesterol Ester Transfer Proteinmedicine.disease_causereverse phase protein arraychemistry.chemical_compoundExonMutationbiologyHomozygotescavenger receptor class B1size exclusion chromatographyLipidCholesteryl ester transfer proteinLipidstorcetrapibApolipoproteinBiochemistryELISAlipids (amino acids peptides and proteins)hyperalphalipoproteinemiaCardiology and Cardiovascular MedicineLipoproteins HDLHumandalcetrapibmedicine.medical_specialtyHeterozygoteDalcetrapibHypercholesterolemiaapolipoproteinhigh-density lipoproteinInternal medicineCholesterylester transfer proteinmedicineAnimalsHumansCholesteryl ester transfer protein; dalcetrapib; high-density lipoprotein; reverse phase protein array; scavenger receptor class B1; size exclusion chromatography; torcetrapib; apolipoprotein; hyperalphalipoproteinemia; ELISAPharmacologybusiness.industryAnimalPoint mutationCholesterol HDLTorcetrapibnutritional and metabolic diseasesLipid MetabolismCholesterol Ester Transfer Proteinscarbohydrates (lipids)Disease Models AnimalEndocrinologyApolipoproteinschemistrybiology.proteinbusinessLipoprotein
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dfh is a Drosophila homolog of the Friedreich's ataxia disease gene

2000

Abstract A putative Drosophila homolog of the Friedreich's ataxia disease gene (FRDA) has been cloned and characterized; it has been named Drosophila frataxin homolog (dfh). It is located at 8C/D position on X chromosome and is spread over 1 kb, a much smaller genomic region than the human gene. Its genomic organization is simple, with a single intron dividing the coding region into two exons. The predicted encoded product has 190 amino acids, being considered a frataxin-like protein on the basis of the sequence and secondary structure conservation when compared with human frataxin and related proteins from other eukaryotes. The closest match between the Drosophila and the human proteins in…

Signal peptideDNA ComplementaryEmbryo NonmammalianMolecular Sequence DataMutantEmbryonic DevelopmentGenes InsectExonIron-Binding ProteinsGeneticsAnimalsDrosophila ProteinsCoding regionAmino Acid SequenceRNA MessengerCloning MolecularGeneIn Situ HybridizationGenomic organizationGeneticsSequence Homology Amino AcidbiologyIntronGene Expression Regulation DevelopmentalDNAExonsSequence Analysis DNAGeneral MedicineBlotting NorthernIntronsPhosphotransferases (Alcohol Group Acceptor)Drosophila melanogasterFriedreich AtaxiaFrataxinbiology.proteinDrosophilaSequence AlignmentGene
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Systematic screening for mutations in the human serotonin-2A (5-HT2A) receptor gene: Identification of two naturally occurring receptor variants and …

1996

A statistically significant association between a silent mutation (102T/C) in the serotonin-2A (5-HT2A) receptor gene and schizophrenia has recently been reported in a sample of Japanese patients and healthy controls. This finding suggests that genetic predisposition to schizophrenia may be affected by a functional 5-HT2A receptor variant that is in linkage disequilibrium with 102T/C. In the present study, we have sought to identify genetic variation in the 5-HT2A receptor gene by screening genomic DNA samples from 91 unrelated subjects comprising 45 patients with schizophrenia and 46 healthy controls by using single-strand conformation analysis. We have identified four nucleotide sequence …

Silent mutationLinkage disequilibriumMolecular Sequence DataRestriction MappingBiologymedicine.disease_causePolymerase Chain ReactionReference ValuesGenetic variationConfidence IntervalsGeneticsGenetic predispositionmedicineHumansPoint MutationReceptor Serotonin 5-HT2AAmino Acid SequenceAlleleAllele frequencyAllelesGenetics (clinical)DNA PrimersGenetic associationGeneticsMutationPolymorphism GeneticBase SequenceChromosomes Human Pair 13Chromosome MappingGenetic VariationExonsReceptors SerotoninSchizophreniaHuman Genetics
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CCDC 1569570: Experimental Crystal Structure Determination

2019

Related Article: Elena Prigorchenko, Sandra Kaabel, Triin Narva, Anastassia Baškir, Maria Fomitšenko, Jasper Adamson, Ivar Järving, Kari Rissanen, Toomas Tamm, Riina Aav|2019|Chem.Commun.|55|9307|doi:10.1039/C9CC04990H

Space GroupCrystallography1310121921283037394648-dodecaazatridecacyclo[46.6.1.1310.11219.12128.13037.13946.049.01318.02227.03136.04045.04954]hexacontane-555657585960-hexone deuterochloroform solvateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 705737: Experimental Crystal Structure Determination

2009

Related Article: D.Meshcheryakov, V.Bohmer, M.Bolte, V.Hubscher-Bruder, F.Arnaud-Neu|2009|Chem.-Eur.J.|15|4811|doi:10.1002/chem.200802573

Space GroupCrystallography97979898-Tetramethyl-3435191-tetra-t-butyl-153147637995-hexaoxa-6822243840545670728688-dodeca-azapentadecacyclo[91.3.1.1^4549^.0^596^.0^914^.0^1621^.0^2530^.0^3237^.0^4146^.0^4853^.0^5762^.0^6469^.0^7378^.0^8085^.0^8994^]octanonaconta-1(96)2491113161820252729323436414345485052575961646668737577808284899193-hexatriacontaene-72339557187-hexone ethanol ethyl acetate solvateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 705736: Experimental Crystal Structure Determination

2009

Related Article: D.Meshcheryakov, V.Bohmer, M.Bolte, V.Hubscher-Bruder, F.Arnaud-Neu|2009|Chem.-Eur.J.|15|4811|doi:10.1002/chem.200802573

Space GroupCrystallographyCrystal SystemCrystal StructureCell Parameters97979898-Tetramethyl-3435191-tetra-t-butyl-153147637995-hexaoxa-6822243840545670728688-dodeca-azapentadecacyclo[91.3.1.1^4549^.0^596^.0^914^.0^1621^.0^2530^.0^3237^.0^4146^.0^4853^.0^5762^.0^6469^.0^7378^.0^8085^.0^8994^]octanonaconta-1(96)2491113161820252729323436414345485052575961646668737577808284899193-hexatriacontaene-72339557187-hexone acetone solvateExperimental 3D Coordinates
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CCDC 1400636: Experimental Crystal Structure Determination

2017

Related Article: Stefania Tomyn, Sergii I. Shylin, Dmytro Bykov, Vadim Ksenofontov, Elzbieta Gumienna-Kontecka, Volodymyr Bon, Igor O. Fritsky|2017|Nat.Commun.|8|14099|doi:10.1038/ncomms14099

Space GroupCrystallographyCrystal Systembis(tetraphenylarsonium) (1347810121316171922-dodeca-azatetracyclo[8.8.4.1317.1812]tetracosane-5614152021-hexonato)-iron(iv) hydrateCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 1400635: Experimental Crystal Structure Determination

2017

Related Article: Stefania Tomyn, Sergii I. Shylin, Dmytro Bykov, Vadim Ksenofontov, Elzbieta Gumienna-Kontecka, Volodymyr Bon, Igor O. Fritsky|2017|Nat.Commun.|8|14099|doi:10.1038/ncomms14099

Space GroupCrystallographybis(tetra-n-butylammonium) (1347810121316171922-dodeca-azatetracyclo[8.8.4.1317.1812]tetracosane-5614152021-hexonato)-iron(iv) chloroform solvateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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