Search results for "Expression"
showing 10 items of 5168 documents
Les microARN, une nouvelle voie de signalisation cellulaire empruntée par le resvératrol
2011
Les microARN (miARN), decouverts en 1993 dans le laboratoire de V. Ambros [1], ont d'abord ete identifies comme regulateurs du developpement chez Caenorhabditis elegans. Les recherches recentes confirment que ces petits ARN non codants simple-brins sont des elements de signalisation cellulaire fondamentaux dans la regulation de processus tels que le developpement, la differenciation ou la proliferation cellulaire. Ces ARN de 22 nucleotides en moyenne s'apparient de facon specifique a des ARN messagers cibles entrainant le blocage de leur traduction (en cas de complementarite parfaite) ou la degradation des transcrits (en cas de un ou quelques mesappariement[s]). Ils pourraient aussi reguler…
The tumor suppressor CYLD controls the function of murine regulatory T cells.
2012
Abstract CYLD was originally identified as a tumor suppressor gene mutated in familial cylindromatosis, an autosomal dominant predisposition to multiple benign neoplasms of the skin known as cylindromas. The CYLD protein is a deubiquitinating enzyme that acts as a negative regulator of NF-κB and JNK signaling through its interaction with NEMO and TNFR-associated factor 2. We have previously described a novel mouse strain that expresses solely and excessively a naturally occurring splice variant of CYLD (CYLDex7/8). In this study, we demonstrate that CYLD plays a critical role in Treg development and function. T cells of CYLDex7/8 mice had a hyperactive phenotype manifested by increased prod…
Expression of phase-specific haemolymph polypeptides in a laboratory strain and field catches of Schistocerca gregaria.
2003
Uvarov's theory of locust phase polymorphism implies a differential phase-specific gene expression. It was the goal of the present investigation to provide data on the molecular level for this concept. We used the technique of 2D gel electrophoresis to generate haemolymph polypeptide maps from mature locust males. Under the given conditions we identified 238 polypeptide spots by their molecular weight and isoelectrical point. Isolated and crowded males from the Mainz strain, all originating from crowded ancesters, differed in 20 spots: three were solitary-specific and 17 crowded-specific. Field catches of solitary and gregarious S. gregaria in Mauritania revealed that those males showed the…
Youth Political Talk in the Changing Media Environment : A Cross-National Typology
2021
While political communication scholarship has long underscored the importance of political talk—casual conversations about news and politics that occur in everyday situations—as a way for citizens to clarify their opinions and as a precursor for political engagement, much of this literature tends to depict political talk as uncomfortable and difficult for citizens. Yet, this focus on the challenging aspects of political talk has been informed predominantly by the US context. To what extent may a different picture emerge when looking across different cultural contexts? And how are these dynamics shaped by the affordances of the multi-platform social media environment? This paper explores th…
Social and sensory deficits in Rett syndrome: neuroanatomical and behavioural analyses in a mouse model deficient for mecp2
2023
El síndrome de Rett es una enfermedad rara del neurodesarrollo que afecta a 1 de cada 10.000 niñas en todo el mundo, y que supone la segunda causa de discapacidad intelectual de origen genético en mujeres. No existe una cura eficaz, y el tratamiento médico es meramente sintomatológico. Sin embargo, esta condición es mucho menos estudiada que otras enfermedades raras que afectan principalmente a niños. Se sabe que el síndrome de Rett está causado principalmente por mutaciones en el gen MECP2, que se localiza en el cromosoma X, haciendo que los niños mueran durante el primer año de vida y las niñas desarrollen la sintomatología característica de Rett a los 6-18 meses de edad. Entre los síntom…
von Hippel-Lindau Protein-Mediated Repression of Tumor Necrosis Factor Alpha Translation Revealed through Use of cDNA Arrays
2003
Based on evidence that the von Hippel-Lindau (VHL) tumor suppressor protein is associated with polysomes and interacts with translation regulatory factors, we set out to investigate the potential influence of pVHL on protein translation. To this end, renal cell carcinoma (RCC) cells that either lacked pVHL or expressed pVHL through stable transfection were used to prepare RNA from cytosolic (unbound) and polysome-bound fractions. Hybridization of cDNA arrays using RNA from each fraction revealed a subset of transcripts whose abundance in polysomes decreased when pVHL function was restored. The tumor necrosis factor alpha (TNF-alpha) mRNA was identified as one of the transcripts that prefere…
NOSIP, a novel modulator of endothelial nitric oxide synthase activity.
2001
Production of nitric oxide (NO) in endothelial cells is regulated by direct interactions of endothelial nitric oxide synthase (eNOS) with effector proteins such as Ca2+-calmodulin, by posttranslational modifications such as phosphorylation via protein kinase B, and by translocation of the enzyme from the plasma membrane caveolae to intracellular compartments. Reversible acylation of eNOS is thought to contribute to the intracellular trafficking of the enzyme; however, protein factor(s) that govern the translocation of the enzyme are still unknown. Here we have used the yeast two-hybrid system and identified a novel 34 kDa protein, termed NOSIP (eNOS interacting protein), which avidly binds …
Innovative pragmatic codes in Ugandan English : a relevance-theoretic account
2013
Published version of an article in the journal: Argumentum. Also available from the publisher at: http://argumentum.unideb.hu/2013-anyagok/bisingoma.pdf Open access The paper investigates innovative pragmatic codes in Ugandan English within the conceptual framework of Relevance Theory (cf. Sperber & Wilson 1986, Wilson & Sperber 2004). Wilson & Sperber (2004) state that an utterance is optimally relevant if it is worth the hearer’s processing effort, and if it is compatible with the speaker’s linguistic abilities and preferences. The reasoning behind these tenets of Relevance Theory can be used to account for the pervasive use of many expressions peculiar to Ugandan English. For example, in…
Heavy metal ion induction of adhesion molecules and cytokines in human endothelial cells: the role of NF-kappaB, I kappaB-alpha and AP-1.
1997
We analyzed the influence of heavy-metal ions on human umbilical vein endothelial cells (HUVEC) in comparison to proinflammatory cytokines (TNF-alpha, IL-1beta) and lipopolysaccharide (LPS). Adhesion molecule and cytokine expressions are upregulated by heavy-metal exposure. Expression of E-selectin on the cell surface was strongly induced by 1-mM concentrations of NiCl2 and CoCl2, whereas ZnCl2 and CrCl3 had no influence. Furthermore, it is shown that NiCl2 induces mRNA expression of E-selectin, intercellular adhesion molecule-1, IL-6 and IL-8 in a 1-mM concentration. The transcription factor NF-kappaB is known to be involved in the regulation of adhesion molecule expression in endothelial …
Analysis of the Biological Response of Endothelial and Fibroblast Cells Cultured on Synthetic Scaffolds with Various Hydrophilic/Hydrophobic Ratios: …
2009
In this study we developed polymer scaffolds intended as anchorage rings for cornea prostheses among other applications, and examined their cell compatibility. In particular, a series of interconnected porous polymer scaffolds with pore sizes from 80 to 110 microns were manufactured varying the ratio of hydrophobic to hydrophilic monomeric units along the polymer chains. Further, the effects of fibronectin precoating, a physiological adhesion molecule, were tested. The interactions between the normal human fibroblast cell line MRC-5 and primary human umbilical vein endothelial cells (HUVECs) with the scaffold surfaces were evaluated. Adhesion and growth of the cells was examined by confocal…