Search results for "Expression"
showing 10 items of 5168 documents
La microdissection laser: une technique d’analyse du profil d’expression génique et protéique des types cellulaires spécifiques de la symbiose mycorh…
2012
Influence des conditions environnementales (statique versus dynamique) sur le développement en biofilm de Listeria monocytogenes EGD-e. Developpement…
2008
National audience
Diagnosis of non target site based resistance within rye grass(Lolium sp): development of quick and reliable tests
2013
Non target site resistance (NTSR) is the major threat for effective herbicide-based control of grass weeds. NTSR, still largely unknown, is endowed by the regulation of a subset of the genes involved in the response of the weed to herbicide treatment. This study aims at identifying NTSR genes in rye-grass to develop resistance diagnosis tests. The transcriptome of plants resistant or sensitive to ALS inhibitors collected before and after herbicide application was sequenced using a deep sequencing technology. We identified genes differentially expressed between resistant and sensitive plants. We developed a RT-qPCR test to measure gene expression levels in individual plants from several popu…
Regulation of gene expression during endomycorrhizal infection
1997
International audience
Caractérisation biochimique, physiologique et moléculaire de différents génotypes de Medicago truncatula
2017
Engineering school; Des études de l’interaction entre la légumineuse modèle Medicago truncatula (Mt) avec l’oomycète Aphanomyces euteiches (Ae) ont montré que la glutamine accumulée dans les racines infectées pourrait être un marqueur de sensibilité à ce pathogène végétal. Pour mieux comprendre le rôle de la glutamine dans la résistance de Mt à Ae,nous avons essayé de doser la glutamine racinaire avec un kit de dosage. D’autre part, nous avons analysé l’impact d’une dose sublétale d’un inhibiteur de la glutamine synthétase (enzyme responsable de la synthèse de glutamine chez les plantes) sur le développement de différents génotypes de Mt. Enfin nous nous sommes intéressés à lacapacité de Mt…
Exploiting Glomus intraradices sequencing data to dissect molecular mechanisms of plant genome control over fungal gene expression in mycorrhiza
2006
International audience
Development of a knowledge graph framework to ease and empower translational approaches in plant research: a use-case on grain legumes
2023
Legumes, and especially pulses, are an important source of protein for food and feed, and are appreciated for their positive impact on the “one health”. However, their unstable yields and their susceptibility to biotic and abiotic stresses highlight the need for varietal improvement in order to increase the cultivated areas and productivity. With the advent of sequencing technologies, a large pool of genetic and -omics resources, heterogeneous at the inter- and intra-species scale, is emerging. Thus, it is important to capitalize on these scattered heterogeneous data to develop translational research to boost breeding projects and crop diversification. To meet this need, we undertook the de…
La combinaison de cartographie de signatures de sélection, de re-séquençage de génomes et d'analyses d'expression révèle PARK2 et JAG2 comme nouveaux…
2015
Very few causal genes have been identified by quantitative trait loci (QTLs) mapping because of the large size ofQTLs, and most of them were identified thanks to functional links already known with the targeted phenotype.Here we propose to combine selection signature detection, SNP annotation, and expression analyses to identifycausal genes underlying QTLs. As a model, we chose experimental chicken lines divergently selected for onlyone trait, the abdominal fat weight, in which several QTLs were previously mapped. Using a new haplotypebasedstatistics exploiting the very high SNP density generated through whole genome re-sequencing, we found129 significant selective sweeps. Most of the QTLs …
Sulfate transporters in the plant’s response to drought and salinity: regulation and possible functions
2014
International audience; Drought and salinity are two frequently combined abiotic stresses that affect plant growth, development, and crop productivity. Sulfate, and molecules derived from this anion such as glutathione, play important roles in the intrinsic responses of plants to such abiotic stresses. Therefore, understanding how plants facing environmental constraints re-equilibrate the flux of sulfate between and within different tissues might uncover perspectives for improving tolerance against abiotic stresses. In this review, we took advantage of genomics and post-genomics resources available in Arabidopsis thaliana and in the model legume species Medicago truncatula to highlight and …
Terminal tendon cell differentiation requires the glide/gcm complex.
2004
International audience; Locomotion relies on stable attachment of muscle fibres to their target sites, a process that allows for muscle contraction to generate movement. Here, we show that glide/gcm and glide2/gcm2, the fly glial cell determinants, are expressed in a subpopulation of embryonic tendon cells and required for their terminal differentiation. By using loss-of-function approaches, we show that in the absence of both genes, muscle attachment to tendon cells is altered, even though the molecular cascade induced by stripe, the tendon cell determinant, is normal. Moreover, we show that glide/gcm activates a new tendon cell gene independently of stripe. Finally, we show that segment p…