Search results for "Expression"
showing 10 items of 5168 documents
RNA Sequencing of Human Peripheral Blood Cells Indicates Upregulation of Immune-Related Genes in Huntington's Disease
2020
Huntington's disease (HD) is an autosomal dominantly inherited neurodegenerative disorder caused by a trinucleotide repeat expansion in the Huntingtin gene. As disease-modifying therapies for HD are being developed, peripheral blood cells may be used to indicate disease progression and to monitor treatment response. In order to investigate whether gene expression changes can be found in the blood of individuals with HD that distinguish them from healthy controls, we performed transcriptome analysis by next-generation sequencing (RNA-seq). We detected a gene expression signature consistent with dysregulation of immune-related functions and inflammatory response in peripheral blood from HD ca…
Iron Induces Proliferation and Morphogenesis in Primmorphs from the Marine SpongeSuberites domuncula
2002
Dissociated cells from marine demosponges retain their proliferation capacity if they are allowed to form special aggregates, the primmorphs. On the basis of incorporation studies and septin gene expression, we show that Fe3+ ions are required for the proliferation of cells in primmorphs from Suberites domuncula. In parallel, Fe3+ induced the expression of ferritin and strongly stimulated the synthesis of spicules. This result is supported by the finding that the enzymatic activity of silicatein, converting organosilicon to silicic acid, depends on Fe3+. Moreover, the expression of a scavenger receptor molecule, possibly involved in the morphology of spicules, depends on the presence of Fe3…
The fnr Gene of Bacillus licheniformis and the Cysteine Ligands of the C-Terminal FeS Cluster
1998
Many of the O2-responsive gene regulators of bacteria are members of the fumarate nitrate reductase-cyclic AMP receptor protein family of transcriptional regulators (12, 13, 15, 17) with predicted structures similar to those of the cyclic AMP receptor protein (11). The Fnr (stands for fumarate nitrate reductase regulator) protein from Escherichia coli (FnrEc) controls the expression of a variety of genes, mainly of anaerobic respiration and metabolism (5, 13). It contains a N-terminal cluster of three essential cysteine residues which are supposed to bind together with Cys122 a [4Fe 4S]2+ cluster which is required for O2 sensing (4, 7, 8, 10, 16). A wide variety of gram-negative bacteria co…
Defective copper transport in the copt5 mutant affects cadmium tolerance.
2014
Cadmium toxicity interferes with essential metal homeostasis, which is a problem for both plant nutrition and the consumption of healthy food by humans. Copper uptake is performed by the members of the Arabidopsis high affinity copper transporter (COPT) family. One of the members, COPT5, is involved in copper recycling from the vacuole toward the cytosolic compartment. We show herein that copt5 mutants are more sensitive to cadmium stress than wild-type plants, as indicated by reduced growth. Exacerbated cadmium toxicity in copt5 mutants is due specifically to altered copper traffic through the COPT5 transporter. Three different processes which have been shown to affect cadmium tolerance ar…
Loss of rod sensitivity and gene expression changes in the retina of fructose-fed insulin-resistant mice
2012
Purpose: Metabolic syndrome is of major concern in Western countries since it predisposes individuals to the development of diabetes. Insulin resistance is one of the biochemical features of the metabolic syndrome. Fructose feeding has been used to elicit insulin resistance in rodents. Our purpose was to characterize the functional and gene expression changes in the retina after long term feeding mice with a fructose-enriched diet.Methods: Control and ApoB100,LDLR-/- mice, a murine model of aging of the human retina, were fed with a 60%-rich fructose diet for 8 months. Scotopic single flash and Flicker electroretinograms were recorded to monitor the response of the retina to flash stimuli. …
Alcohol Abuse Mediates the Association between Baseline T/C Ratio and Anger Expression in Intimate Partner Violence Perpetrators.
2015
The imbalance between testosterone (T) and cortisol (C) levels has been proposed as a possible marker of risk for intimate partner violence (IPV). Moreover, it could be related to a high probability of adopting risky behaviors such as alcohol abuse which, in turn, promotes the onset of IPV. This study tested the potential mediating effect of alcohol consumption on the relationship between baseline T/C ratio and anger expression in IPV perpetrators and non-violent controls. Alcohol consumption was higher in the former than controls. A high baseline T/C ratio was only associated with high anger expression in IPV perpetrators, and this association was mediated by high alcohol consumption. Thus…
Cell and molecular response to IORT treatment
2014
Ionizing radiations (IRs) generated by intraoperative radiotherapy (IORT) treatment activates both pro- and antiproliferative signal pathways producing an imbalance in cell fate decision regulated by several genes and factors involved in cell cycle progression, survival and/or cell death, DNA repair and inflammation. This paper describes the latest advances on cellular and molecular response to IR, highlighting the most relevant research data from cell biology, gene expression profiling and epigenetic studies on different tumor cell types. Understanding the cell molecular strategies to choose between death and survival, after an irradiation-induced damage, opens new avenues for the selectio…
Varhaisnuorten emootiot suoriutumistilanteissa : taitotasoltaan erilaisten kuudesluokkalaisten perustunteiden tunnistaminen objektiivisesti ja subjek…
2017
Tässä tutkimuksessa on tarkasteltu varhaisnuorten tunteiden tunnistamista suoriutumistilanteissa. Subjektiivisesti ja objektiivisesti mitattujen tunteiden vastaavuuksia tarkasteltiin ottaen huomioon tehtävien vaikeustaso ja esittämisjärjestys, oppilaan kognitiivinen kykytaso, ylivilkkauden pulmat sekä tunteiden huippuhetket ja aallonpohjat. Tutkimus on toteutettu osana syksyllä 2013 käynnistynyttä TIKAPUU – Alakoulusta yläkouluun – tutkimushanketta, johon osallistuu 55 koululuokkaa kahdesta Keski-Suomen kunnasta. Tutkimuksen aineisto (n = 154) koostuu yksilötutkimuksiin osallistuneista nuorista, joiden kasvonilmeitä mitattiin FaceReader-ohjelman avulla heidän tehdessään yleisen päättelyn te…
“F*** this shit” : Negotiating the Boundaries of Public Expression of Mother’s Negative Feelings
2021
In 2017, Finnish celebrity Sini Ariell published a blog post titled “F*** this shit” in which she revealed her difficult feelings as a mother of a newborn baby. Attracting attention from Finnish media, the post went viral. Through the empirical analysis of affective reactions to the post on an anonymous online discussion board, we explore how the limits of the public expression of negative maternal feelings are negotiated and maintained, but also challenged. Our results highlight the normative, even punitive dynamics of the digital intimate public, as commenters on the discussion board often argued that struggles and unhappiness during motherhood should not be made public but dealt with in …