Search results for "Eye"

Surveillance of a municipal drinking-water supply after a Norovirus outbreak in Italy

2012

Expression of Phosphofructokinase Is Not Sufficient to Enable Embden-Meyerhof-Parnas Glycolysis in Zymomonas mobilis ZM4

2019

Zymomonas mobilis is a bacterium that produces ethanol from glucose at up to 97% of theoretical efficiency on a carbon basis. One factor contributing to the high efficiency of ethanol production is that Z. mobilis has a low biomass yield. The low biomass yield may be caused partly by the low ATP yield of the Entner-Doudoroff (ED) glycolytic pathway used by Z. mobilis, which produces only one ATP per glucose consumed. To test the hypothesis that ATP yield limits biomass yield in Z. mobilis, we attempted to introduce the Embden-Meyerhof-Parnas (EMP) glycolytic pathway (with double the ATP yield) by expressing phosphofructokinase (Pfk I) from Escherichia coli. Expression of Pfk I caused growth…

Accommodation-related changes in monochromatic aberrations of the human eye as a function of age.

2008

PURPOSE. To investigate the relationship between accommodation and the optical aberrations of the whole human eye, as a function of age. METHODS. Sixty healthy subjects with spherical ametropia in the range 3 D, astigmatism less than 1 D, corrected visual acuity of 20/18 or better, and normal findings in an ophthalmic examination were enrolled. Subjects were divided into four groups, with age ranges of 19 to 29, 30 to 39, 40 to 49, and 50 to 60 years. Monochromatic optical aberrations and pupil size were measured with a Hartmann-Shack wavefront sensor under monocular viewing conditions, without pharmacological dilation or cycloplegia. Stimulus vergences were in the range of 0 to 5 D, with a…

Possible involvement of nitric oxide in morphine-induced miosis and reduction of intraocular pressure in rabbits.

2006

The role of μ3 opioid receptors in morphine-induced intraocular pressure (IOP) lowering effect and miosis was evaluated in conscious, dark-adapted New Zealand white (NZW) rabbits using a masked-design study. IOP and pupil diameter (PD) measurements were taken at just before and 0.5, 1, 2, 4, 6 h after monolateral instillation of morphine (10, 50 and 100 μg/30 μl) as compared to vehicle administered in the contralateral eye. Morphine-induced ocular effects were challenged by a pre-treatment with the non-selective opioid receptor antagonist, naloxone (100 μg/30 μl), the nitric oxide synthase inhibitor, Nω-nitro-l-arginine methyl ester (l-NAME, 1%, 30 μl), or the non-selective μ3 opioid recept…

Retinal straylight in patients with monofocal and multifocal intraocular lenses

2007

Purpose To determine the differences in retinal straylight perceived by patients with monofocal intraocular lenses (IOLs) and patients with multifocal IOLs. Setting Vissum-Instituto de Oftalmologico de Alicante, Alicante, Spain. Methods In this prospective study, contrast sensitivity and retinal straylight were measured prospectively 6 months postoperatively in 67 eyes of 40 patients with a monofocal or multifocal IOL. In the monofocal group, a ThinOptX IOL (ThinOptX, Inc.) was implanted in 12 eyes and an Acri.Smart 48 S IOL (Acri.Tec) in 20 eyes. In the multifocal IOL group, a ReZoom IOL (Advanced Medical Optics) was implanted in 13 eyes and a ReSTOR IOL (Alcon Laboratories) in 22 eyes. Re…

Occult non-metallic intraocular foreign body causing recurrent anterior uveitis

2015

Purpose To describe the case of textile worker with recurrent anterior uveitis following the traumatic penetration of nylon thread into the anterior segment of the eye. Methods Examination by slit-lamp of the affected eye B-scan ultrasonography during the inflammatory episode and after the resolution of the uveitis. Visual Acuity Measurement during the inflammatory episode and after the resolution of the uveitis. Results The use of topical therapy together with antibiotic systemic therapy led to the regression of the inflammatory symptoms (intense epibulbar and tarsal hyperemia with perikeratic reaction, corneal sub-oedema with some folds in the Descemet's membrane, Tyndall 2+ and a layer o…

Statistical Validation of the Identification of Tuna Species:  Bootstrap Analysis of Mitochondrial DNA Sequences

2002

Sequencing of the mitochondrial cytochrome b gene has been used to differentiate three tuna species: Thunnus albacares (yellowfin tuna), Thunnus obesus (bigeye tuna), and Katsuwonus pelamis (skipjack). A PCR amplified 528 bp fragment from 30 frozen samples and a 171 bp fragment from 26 canned samples of the three species were analyzed to determine the intraspecific variation and the positions with diagnostic value. Polymorphic sites between the species that did not present intraspecific variation were given a diagnostic value. The genetic distance between the sequences was calculated, and a phylogenetic tree was constructed, showing that the sequences belonging to the same species clustered…

Anisotropic Elasto-optic Effect in Optical Fibers under Axial Strain: Experimental Observation by means of Whispering Gallery Modes Resonances

2014

Experimental characterization of the refractive index anisotropy generated by the elasto optic effect in a conventional optical fiber under axial strain by using WGMs resonances and their tunability with strain.

Imaging quality of multifocal intraocular lenses: automated assessment setup

2012

[EN] Purpose: A new technique for the assessment of the optical quality of multifocal intraocular lenses (MIOLs) under monochromatic and polychromatic illumination is presented. Methods: The system provides, in a totally automated procedure, the modulation transfer function (MTF) of the lens under test for different axial positions of the object. The artificial eye admits different artificial corneas, to optimise the axial resolution in the sampling of the MTF of the MIOL under test, and different pupils, to test the dependence of the optical performance of the MIOL on the eye pupil diameter. Results: The performance and sensitivity of the apparatus is tested with different commercial MIOLs…

Recurrent Mutations in the Basic Domain of TWIST2 Cause Ablepharon Macrostomia and Barber-Say Syndromes

2015

Contains fulltext : 153827.pdf (Publisher’s version ) (Open Access) Ablepharon macrostomia syndrome (AMS) and Barber-Say syndrome (BSS) are rare congenital ectodermal dysplasias characterized by similar clinical features. To establish the genetic basis of AMS and BSS, we performed extensive clinical phenotyping, whole exome and candidate gene sequencing, and functional validations. We identified a recurrent de novo mutation in TWIST2 in seven independent AMS-affected families, as well as another recurrent de novo mutation affecting the same amino acid in ten independent BSS-affected families. Moreover, a genotype-phenotype correlation was observed, because the two syndromes differed based s…