Search results for "Eye"
showing 10 items of 2511 documents
Immunohistochemical analysis of vascular endothelial growth factor (VEGF) and p53 expression in pterygium from Tunisian patients.
2009
A pterygium is characterized by abnormal fibrovascular corneoconjunctival tissue. A number of investigations have attempted to elucidate this incompletely understood pathology. Since vascular endothelial growth factor (VEGF) and p53 are known to participate in tumor vascularization, our purpose was to study VEGF and p53 expression in active primary and recurrent pterygium from Tunisian patients. To this end, 15 cases of active primary pterygium and five cases of recurrent pterygium from Tunisia were studied by immunohistochemistry. Antibodies raised against VEGF and p53 were used to analyze the distribution and expression of these markers in pterygium and normal human conjunctiva were used …
Immunohistochemical analysis of cellular adhesion molecules (ICAM-1, VCAM-1) and VEGF in fibrovascular membranes of patients with proliferative diabe…
2009
Abstract Purpose Diabetic fibrovascular membranes are the main pathological changes of proliferative diabetic retinopathy that can cause serious complications leading to blindness. Since the mechanism of fibrovascular membrane development is still unknown, the aim of our study was to identify potential biomarkers for this pathology. To this end, we analyzed the simultaneous expression of ICAM-1, VCAM-1 and VEGF within tissues of diabetic fibrovascular membranes. Patients and methods Fibrovascular membranes were taken from nine diabetic patients with proliferative diabetic retinopathy. The fibrovascular membrane specimens were analyzed by immunohistochemistry to determine ICAM-1, VCAM-1 and …
Effects of a nutraceutical formulation based on the combination of antioxidants and ω-3 essential fatty acids in the expression of inflamma…
2013
Maria D Pinazo-Durán,1,* Carmen Galbis-Estrada,1,* Sheila Pons-Vázquez,1 Jorge Cantú-Dibildox,2 Carla Marco-Ramírez,1 Javier Benítez-del-Castillo21Ophthalmic Research Unit Santiago Grisolia, Department of Surgery/Ophthalmology, Faculty of Medicine, University of Valencia, Valencia, Spain; 2Department of Ophthalmology, Hospital of Jerez, Jerez de la Frontera, Spain*These authors contributed equally to this workBackground: Women, and those older than 65 years of age, are particularly susceptible to dry eye disorders (DEDs). Inflammation is clearly involved in the pathogenesis of DEDs, and there is mounting evidence on the antioxidant and …
Aqueous Humor Levels of Vascular Endothelial Growth Factor in Retinitis Pigmentosa
2008
PURPOSE To determine the level of vascular endothelial growth factor A (VEGF-A) in aqueous humors of patients with retinitis pigmentosa (RP). METHODS A prospective, comparative control study. Aqueous humor was collected from 16 eyes of 16 patients with RP. The level of VEGF-A was determined with a commercially available enzyme-linked immunosorbent assay kit. The control group comprised 16 aqueous samples from 16 patients about to undergo cataract surgery and without any other ocular or systemic diseases. RESULTS The concentration of VEGF-A in aqueous humor was markedly lower in patients with RP than in control subjects (Mann-Whitney U test, P < 0.001). The level of VEGF-A was 94.9 +/- 99.8 …
Elevated protein carbonyl and HIF-1α levels in eyes with proliferative diabetic retinopathy.
2013
Purpose: To evaluate the role of protein carbonyls and hypoxia inducible factor-1a (HIF-1a) in diabetic eyes with proliferative diabetic retinopathy (PDR). Methods: Prospective consecutive controlled observational study was performed. Vitreous samples were collected at the start of the 3-ppp vitrectomy. Protein carbonylation analysis was performed by Western blotting with antibody against 2,4-Dinitrophenol (anti-DNP), following derivatization of protein carbonyls with 2,4 Dinitrophenylhydrazine (DNHP). Protein carbonylation was quantified by scanning densitometry analysis and relativized to the total amount of protein into the ponceau staining of membranes. Vitreous HIF-1 a was determined w…
Monocular Versus Binocular Calibrations in Evaluating Fixation Disparity With a Video-Based Eye-Tracker
2015
When measuring fixation disparity (an oculomotor vergence error), the question arises as to whether a monocular or binocular calibration is more precise and physiologically more appropriate. In monocular calibrations, a single eye fixates on a calibration target that is taken as having been projected onto the center of the fovea; the corresponding vergence state represents the heterophoria (the resting vergence position), which has no effect on the calibration procedure. In binocular calibrations, a vergence error may be present and may affect the subsequent measurement of the fixation disparity during binocular recordings. This study includes a test of the precision of both monocular and …
Assessment of corneal morphological changes induced by the use of daily disposable contact lenses.
2015
To assess the effect of different disposable soft contact lenses upon corneal thickness, and upon anterior and posterior corneal curvatures using a dual-Scheimpflug imaging based device.Twenty-eight young, healthy subjects wore four different types of daily disposable soft contact lenses on four different days: Dailies Total1, Proclear 1 Day, Clariti 1-Day and 1-Day Acuvue Moist. The lenses had different material and water content. Pachymetry maps and keratometry values were obtained using the Galilei G4 twice a day: one before putting the lens on and one after an eight-hour period of contact-lens wear. Measurements were also recorded without any contact lenses being worn during a day.Clari…
Long-term comparison of corneal aberration changes after laser in situ keratomileusis: mechanical microkeratome versus femtosecond laser flap creatio…
2010
Purpose To compute and compare visual acuity, refractive outcomes, and anterior corneal aberration changes after myopic laser in situ keratomileusis (LASIK) with flap creation by a mechanical microkeratome and by a femtosecond laser. Setting Private practice refractive surgery center, Valencia, Spain. Design Comparative case series. Methods Patients were assigned to have LASIK flap creation with a mechanical microkeratome (Carriazo-Barraquer) or a femtosecond laser (IntraLase). The Visx S2 excimer laser was used for myopic ablation in all cases. Main outcome measures included uncorrected and corrected distance visual acuities and the defocus equivalent. Higher-order aberrations (HOAs) were …
Influence of different types of astigmatism on visual acuity
2016
[EN] Purpose To investigate the change in visual acuity (VA) produced by different types of astigmatism (on the basis of the refractive power and position of the principal meridians) on normal accommodating eyes. Methods The lens induced method was employed to simulate a set of 28 astigmatic blur conditions on different healthy emmetropic eyes. Additionally, 24 values of spherical defocus were also simulated on the same eyes for comparison. VA was measured in each case and the results, expressed in logMAR units, were represented against of the modulus of the dioptric power vector (blur strength). Results LogMAR VA varies in a linear fashion with increasing astigmatic blur, being the slope o…
Visual performance of two simultaneous vision multifocal contact lenses
2012
Purpose To evaluate and compare the visual performance of two simultaneous vision multifocal contact lenses (CLs). Methods In this cross-over study design 20 presbyopic subjects were fitted with two different simultaneous vision multifocal CLs (the PureVision Multifocal Low Add and Acuvue Oasys for Presbyopia) in random order. After 1 month, binocular distance visual acuity (BDVA) under photopic (85 cd/m2) and mesopic (3 cd/m2) conditions, binocular near visual acuity (BNVA), binocular distance contrast sensitivity function (CSF) under photopic and mesopic conditions, binocular near CSF and defocus curve were measured. Subjects were then refitted with the alternative correction and the proc…