Search results for "FAD"

Rho protein inactivation induced apoptosis of cultured human endothelial cells.

2002

Small GTP-binding Rho GTPases regulate important signaling pathways in endothelial cells, but little is known about their role in endothelial cell apoptosis. Clostridial cytotoxins specifically inactivate GTPases by glucosylation [ Clostridium difficile toxin B-10463 (TcdB-10463), C. difficile toxin B-1470 (TcdB-1470)] or ADP ribosylation ( C. botulinum C3 toxin). Exposure of human umbilical cord vein endothelial cells (HUVEC) to TcdB-10463, which inhibits RhoA/Rac1/Cdc42, or to C3 toxin, which inhibits RhoA, -B, -C, resulted in apoptosis, whereas inactivation of Rac1/Cdc42 with TcdB-1470 was without effect, suggesting that Rho inhibition was responsible for endothelial apoptosis. Disruptio…

Amyloid Beta-Mediated Changes in Synaptic Function and Spine Number of Neocortical Neurons Depend on NMDA Receptors

2021

Onset and progression of Alzheimer’s disease (AD) pathophysiology differs between brain regions. The neocortex, for example, is a brain region that is affected very early during AD. NMDA receptors (NMDARs) are involved in mediating amyloid beta (Aβ) toxicity. NMDAR expression, on the other hand, can be affected by Aβ. We tested whether the high vulnerability of neocortical neurons for Aβ-toxicity may result from specific NMDAR expression profiles or from a particular regulation of NMDAR expression by Aβ. Electrophysiological analyses suggested that pyramidal cells of 6-months-old wildtype mice express mostly GluN1/GluN2A NMDARs. While synaptic NMDAR-mediated currents are unaltered in 5xFAD …

Scattering resonances and Pseudospectrum : stability and completeness aspects in optical and gravitational systems

2022

The general context of this thesis is an effort to establish a bridge between gravitational andoptical physics, specifically in the context of scattering problems using as a guideline concepts andtools taken from the theory of non-self-adjoint operators. Our focus is on Quasi-Normal Modes(QNMs), namely the natural resonant modes of open leaky structures under linear perturbationssubject to outgoing boundary conditions. They also are referred to as scattering resonances.In the conservative self-adjoint case the spectral theorem guarantees the completeness andspectral stability of the associated normal modes. In this sense, a natural question in the non-self-adjoint setting refers to the char…

Pressurized liquid extraction combined with capillary electrophoresis–mass spectrometry as an improved methodology for the determination of sulfonami…

2007

A new analytical method, based on capillary electrophoresis and tandem mass spectrometry (CE-MS2), is proposed and validated for the identification and simultaneous quantification of 12 sulfonamides (SAs) in pork meat. The studied SAs include sulfathiazole, sulfadiazine, sulfamethoxypyridazine, sulfaguanidine, sulfanilamide, sulfadimethoxyne, sulfapyridine, sulfachloropyridazine, sulfisoxazole, sulfasalazine, sulfabenzamide and sulfadimidine. Different parameters (i.e. separation buffer, sheath liquid, electrospray conditions) were optimized to obtain an adequate CE separation and high MS sensitivity. MS2 experiments using an ion trap as analyzer, operating in the selected reaction monitori…

Nucleon Form Factors at high q2 within constituent quark models

2000

The nucleon form factors are calculated using a non-relativistic description in terms of constituent quarks. The emphasis is put on the reliability of present numerical methods used to solve the three-body problem in order to correctly reproduce the expected asymptotic behavior of form factors. Nucleon wave functions obtained in the hyperspherical formalism or employing Faddeev equations have been considered. While a q**(-8) behavior is expected at high q for a quark-quark force behaving like 1/r at short distances, it is found that the hypercentral approximation in the hyperspherical formalism (K=0) leads to a q**(-7) behavior. An infinite set of waves is required to get the correct behavi…

Charmed and Bottom Baryons: a Variational Approach based on Heavy Quark Symmetry

2003

The use of Heavy Quark Symmetry to study bottom and charmed baryons leads to important simplifications of the non-relativistic three body problem, which turns out to be easily solved by a simple variational ansatz. Our simple scheme reproduces previous results (baryon masses, charge and mass radii, $...$) obtained by solving the Faddeev equations with simple non-relativistic quark--quark potentials, adjusted to the light and heavy--light meson spectra. Wave functions, parameterized in a simple manner, are also given and thus they can be easily used to compute further observables. Our method has been also used to find the predictions for strangeness-less baryons of the SU(2) chirally inspire…

SAHA/TRAIL combination induces detachment and anoikis of MDA-MB231 and MCF-7 breast cancer cells

2012

Abstract SAHA, an inhibitor of histone deacetylase activity, has been shown to sensitize tumor cells to apoptosis induced by TRAIL, a member of TNF-family. In this paper we investigated the effect of SAHA/TRAIL combination in two breast cancer cell lines, the ERα−positive MCF-7 and the ERα−negative MDA-MB231. Treatment of MDA-MB231 and MCF-7 cells with SAHA in combination with TRAIL caused detachment of cells followed by anoikis, a form of apoptosis which occurs after cell detachment, while treatment with SAHA or TRAIL alone did not produce these effects. The effects were more evident in MDA-MB231 cells, which were chosen for ascertaining the mechanism of SAHA/TRAIL action. Our results show…

An experimental testbed of an Internet of Underwater Things

2020

A number of critical features have so far slowed down the realization of an Internet of Underwater Things. The most relevant of these aspects are related to the unreliability of the communication channel, the long propagation delay and the effect of severe multi-path and fading. This paper presents the design and development of a hybrid underwater-terrestrial IoT where different underwater sensors collect heterogeneous data and use marine acoustic modems to send information to a gateway device; this is able to set up a long distance link, implemented through a LoRaWAN connection, to forward data to a remote cloud for further processing. Performance of this system has been tested in a real s…

La abominación que no cesa

2009

Throughput and energy efficiency comparison of one-hop, two-hop, virtual relay and cooperative retransmission schemes

2010

Two main types of approaches exist for implementing cooperative communications at the MAC layer: virtual-hop relay and cooperative retransmission. While the virtual-hop relay schemes employ relay nodes to forward packets when higher end-to-end throughput can be achieved compared with the direct transmission, the cooperative retransmission schemes use relays to retransmit data only after the direct transmission fails. However, the performance of the these different approaches has not been compared in the literature, especially when energy efficiency is considered. In order to find out the best transmission scheme, this paper evaluates and compares the performance of the one-hop direct transm…