Search results for "FAS"
showing 10 items of 2015 documents
Cardiomyocyte apoptosis is related to left ventricular dysfunction and remodelling in dilated cardiomyopathy, but is not affected by growth hormone t…
2007
Background and aims Cardiomyocyte apoptosis (CA) is a common feature of end-stage heart failure. We examined whether CA is associated with cardiac dysfunction and remodelling in heart failure due to dilated cardiomyopathy and studied the effect of human growth hormone (hGH) on CA. Methods and results We studied 38 patients, included in a phase III multi-center, randomised, double-blind and placebo-controlled trial of biosynthetic hGH treatment in dilated cardiomyopathy, at baseline and after 14 weeks treatment. Twenty-six patients received hGH and 12 received placebo. CA was quantified in endomyocardial biopsies using the TUNEL assay. CA correlated with left ventricular size (r=0.43, p=0.00…
Tra «pentoloni per il solfato ammonico» e «un numero inverosimile di tubi»: le lettere di Carlo Emilio Gadda all’Ammonia Casale
2022
The essay analyzes the correspondence between Carlo Emilio Gadda and the "Ammonia Casale S.A." which intermittently covers a period from April 1927 to January 1940, with a long break between the autumn of 1931 and the spring of 1936. It is a decisive period of time on the public and private level. Reading the correspondence allows you to retrace an entire page of the history of Italian industry under fascism from an eccentric perspective.
Modified electrocardiograph-triggered black-blood turbo spin-echo technique to improve T1-weighting in contrast-enhanced MRI of atherosclerotic carot…
2008
Purpose To assess the efficacy of a modified electrocardiograph (EKG)-triggered black-blood T1W (T1W) spin-echo sequence in improving contrast on post-gadolinium high-resolution carotid plaque imaging by implementing heart-rate–independent contrast preparation. Materials and Methods We used a standard EKG-triggered double inversion-recovery (DIR) turbo spin-echo (TSE) sequence modified with the addition of an extra saturation (90°) radio frequency (RF) pulse placed immediately after the DIR module, shortening the repetition time to a fixed value of 400 msec. A total of 10 patients with atherosclerotic disease were included in the study. Postinjection intraplaque contrast measurements were p…
Cartoon filter via adaptive abstraction
2016
We propose a non-parametric methodology to realize abstraction images.The redundant wavelet "a trous" algorithm is applied for details detection.An multi-scale circular median filter is used as a smoothing filter.The proposed algorithm is simple and fast on low-cost entry-level hardware. Abstraction in computer graphics defines a procedure that discriminates the essential information that is worth keeping. Usually details, that correspond to higher frequency components, allow to distinguish otherwise similar images. Vice versa, low frequencies are related to the main information, which are larger structures. Contours themselves may also be identified by high frequencies and separate each pi…
Reversible stress-induced lipid body formation in fast twitch rat myofibers
2012
We analyzed the existence of lipid bodies (LBs) in the fast twitch rat flexor digitorum brevis (FDB) myofibers and found that these structures were scarce. However, isolation procedure of the myofibers, heath shock, viral infection or the glycosylation inhibitor tunicamycin induced formation of the LBs, which were stationary structures flanking Z lines. We next infected FDB myofibers with recombinant Semliki Forest virus expressing caveolin 3-yellow fluorescent protein (cav3-YFP) since this chimeric protein was targeted to the LBs facilitating their further analysis. Photobleaching experiments showed that the LBs recovered cav 3-YFP extremely slowly, indicating that they were not continuous…
A Golgi study of the principal projection neurons of the medial cortex of the lizardPodarcis hispanica
1997
The medial cortex of lizards is a simple three-layered brain region displaying many characteristics that parallel the hippocampal fascia dentata of mammals. Its principal neurons form a morphologically diverse population, partly as a result of the prominent continuous growth of this nervous center. By using the classic Golgi impregnation method, we describe here the morphology of the principal neurons populating the medial cortex of Podarcis hispanica. These were projection neurons giving off descending axons. These axons displayed deep collateral branches provided with prominent axonal boutons, while the main axonal branch reached adjacent cortical areas and the bilateral septum. According…
Increased stability of the TM helix oligomer abrogates the apoptotic activity of the human Fas receptor
2021
Human death receptors control apoptotic events during cell differentiation, cell homeostasis and the elimination of damaged or infected cells. Receptor activation involves ligand-induced structural reorganizations of preformed receptor trimers. Here we show that the death receptor transmembrane domains only have a weak intrinsic tendency to homo-oligomerize within a membrane, and thus these domains potentially do not significantly contribute to receptor trimerization. However, mutation of Pro183 in the human CD95/Fas receptor transmembrane helix results in a dramatically increased interaction propensity, as shown by genetic assays. The increased interaction of the transmembrane domain is co…
Mutations in spalt cause a severe but reversible neurodegenerative phenotype in the embryonic central nervous system ofDrosophila melanogaster
2002
The gene spalt is expressed in the embryonic central nervous system of Drosophila melanogaster but its function in this tissue is still unknown. To investigate this question, we used a combination of techniques to analyse spalt mutant embryos. Electron microscopy showed that in the absence of Spalt, the central nervous system cells are separated by enlarged extracellular spaces populated by membranous material at 60% of embryonic development. Surprisingly, the central nervous system from slightly older embryos (80% of development) exhibited almost wild-type morphology. An extensive survey by laser confocal microscopy revealed that thespalt mutant central nervous system has abnormal levels o…
Lymphocyte apoptosis in children with central nervous system tuberculosis: a case control study
2011
Abstract Background Studies of the apoptosis mechanisms involved in the pathogenesis of tuberculosis have suggested that Mycobacterium tuberculosis can actively interfere with the apoptosis of infected cells. In vivo studies have been performed in adult populations but have not focused on this process in children. In the present study, we analyzed spontaneous T lymphocyte (PBT) apoptosis in the peripheral blood of children with central nervous system tuberculosis (CNS TB), before and after chemotherapy, and compared the results with healthy controls. Methods A case-control study was conducted from January 2002 to June 2009. It included 18 children with CNS TB and 17 healthy controls. Sponta…
Anandamide-induced apoptosis in Chang liver cells involves ceramide and JNK/AP-1 pathway
2006
In the present study we demonstrate that anandamide, the most important endogenous cannabinoid, markedly induced apoptosis in Chang liver cells, an immortalized non-tumor cell line derived from normal liver tissue, while it induced only modest effects in a number of hepatoma cell lines. The apoptotic effect was reduced by methyl-beta-cyclodextrin, a membrane cholesterol depletor, suggesting an interaction between anandamide and the membrane microdomains named lipid rafts. Anandamide effects were mediated by the production of ceramide, as demonstrated by experiments performed with the sphingomyelinase inhibitor, desipramine, or with the sphingomyelinase activator, melittin. This conclusion w…