Search results for "FATE"

showing 10 items of 765 documents

A novel cell wall protein specific to the mycelial form of Yarrowia lipolytica.

1996

A cDNA clone specifying a cell wall protein was isolated from a Yarrowia lipolytica cDNA library. The cDNA library was constructed in the expression vector lambda gt 11, with the RNA isolated from actively growing mycelial cells. The deduced amino acid sequence shows that the encoded protein contains an N-terminal hydrophobic signal peptide. We have designated this protein YWP1 for Yarrowia lipolytica cell Wall Protein. Northern hybridization identified YWP1 transcript only when Y. lipolytica was growing in the mycelial form. The encoded protein seems to be covalently bound to the glucan cell wall since it is not released from the cell walls by sodium dodecyl sulphate extraction, but it is …

Signal peptideDNA ComplementaryTranscription GeneticHydrolasesBlotting WesternGenetic VectorsMolecular Sequence DataRestriction MappingBioengineeringApplied Microbiology and BiotechnologyBiochemistryCell wallFungal ProteinsOpen Reading FramesTransformation GeneticCell WallComplementary DNAGene Expression Regulation FungalYeastsGeneticsEscherichia coliAmino Acid SequenceCloning MolecularFluorescent Antibody Technique IndirectPeptide sequenceAntibodies FungalGene LibraryExpression vectorbiologyBase SequencecDNA libraryRNASodium Dodecyl SulfateYarrowiaRNA Fungalbiology.organism_classificationBlotting NorthernBlotting SouthernBiochemistrySaccharomycetalesElectrophoresis Polyacrylamide GelBiotechnologyYeast (Chichester, England)
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A study of the Candida albicans cell wall proteome

2008

Considering the importance of proteins in the structure and function of the cell wall of Candida albicans, we analyzed the cell wall subproteome of this important human pathogen by LC coupled to MS (LC-MS) using different protein extraction procedures. The analyzed samples included material extracted by hydrogen fluoride-pyridine (HF-pyridine), and whole SDS-extracted cell walls. The use of this latter innovative procedure gave similar data as compared to the analysis of HF-pyridine extracted proteins. A total of 21 cell wall proteins predicted to contain a signal peptide were identified, together with a high content of potentially glycosylated Ser/Thr residues, and the presence of a GPI mo…

Signal peptideProteomebiologyPyridinesSodium Dodecyl SulfateProteomicsbiology.organism_classificationBiochemistryHydrofluoric AcidCorpus albicansFungal ProteinsCell wallBiochemistryCell WallTandem Mass SpectrometryCandida albicansProteomeProtein purificationSolventsBlastosporeCandida albicansMolecular BiologyPROTEOMICS
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Catalytic Functionalization of Methane and Light Alkanes in Supercritical Carbon Dioxide

2014

International audience; The development of catalytic methods for the effective functionalization of methane yet remains a challenge. The best system known to date is the so-called Catalytica Process based on the use of platinum catalysts to convert methane into methyl bisulfate with a TOF rate of 10−3 s. In this contribution, we report a series of silver complexes containing perfluorinated tris(indazolyl)borate ligands that catalyze the functionalization of methane into ethyl propionate upon reaction with ethyl diazoacetate (EDA) by using supercritical carbon dioxide (scCO2) as the reaction medium. The employment of this reaction medium has also allowed the functionalization of ethane, prop…

SilverLigands010402 general chemistry01 natural sciences7. Clean energyCatalysisCatalysischemistry.chemical_compoundEthyl propionateEthyl diazoacetateCoordination ComplexesMethyl bisulfateAlkanesOrganic chemistry[CHIM.COOR]Chemical Sciences/Coordination chemistrySupercritical carbon dioxide010405 organic chemistryOrganic ChemistryButaneDiazonium CompoundsGeneral ChemistryCarbon Dioxide0104 chemical sciencesSolubilitychemistryIsobutaneOxidative coupling of methaneMethaneChemistry - A European Journal
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A mathematical approach to predicting the percutaneous absorption enhancing effect of sodium lauryl sulphate.

2003

A study has been made of the effect of sodium lauryl sulphate (SLS) at several concentrations from 0.24 to 5% (w/w) on skin permeability. Seven model drugs were selected for this study on the basis of their lipophilicity as represented by their logP(oct) values (from -0.95 to 4.2). Skin pre-treatment with aqueous solutions of SLS does not increase the permeability coefficient of the most lipophilic compounds (logP(oct)> or =3). For the other compounds assayed the increase in the permeability coefficients depends on the concentration of SLS used in the skin pre-treatment, and on the lipophilicity of the compounds tested.The correlation between the inverse of SLS efficacy as an enhancer (1/ER…

Skin AbsorptionPharmaceutical ScienceAbsorption (skin)In Vitro TechniquesAdministration CutaneousModels Biologicalchemistry.chemical_compoundSurface-Active AgentsPharmacokineticsAnimalsPharmacokineticsSodium dodecyl sulfateRats WistarMathematical ComputingAqueous solutionChromatographyintegumentary systemChemistrySodium lauryl sulphateSodium Dodecyl SulfateRatsPharmaceutical PreparationsPermeability (electromagnetism)LipophilicityFemaleAzoneAlgorithmsInternational journal of pharmaceutics
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LC of high to moderately polar basic drugs in urine with water and detergent, and direct injection

2016

Background: Micellar LC was first proposed as a ‘green’ mode using mobile phases of water and surfactant. However, in most procedures a small amount of organic solvent is required to decrease the retention to convenient values. Results & methodology: Mixed micellar mobile phases prepared with both cationic (sodium dodecyl sulphate) and nonionic surfactant (Brij-35) modulate the retention of high to moderately polar basic drugs to practical times, eliminating the need of organic solvent. While the mobile phase is continuously recycled through the system, the stationary phase performance is maintained after repetitive injection of the samples. Discussion & conclusion: Through an exte…

SodiumClinical Biochemistrychemistry.chemical_elementUrine010402 general chemistry01 natural sciencesPolyethylene GlycolsAnalytical ChemistrySurface-Active AgentsPulmonary surfactantLimit of DetectionPhase (matter)HumansGeneral Pharmacology Toxicology and PharmaceuticsMicellesChromatographyChemistryOrganic solvent010401 analytical chemistryCationic polymerizationSodium Dodecyl SulfateWaterGeneral Medicine0104 chemical sciencesMedical Laboratory TechnologyPharmaceutical PreparationsStationary phaseFlow Injection AnalysisPolarChromatography LiquidBioanalysis
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Comparison of adsorption equilibrium models and error functions for the study of sulfate removal by calcium hydroxyapatite microfibrillated cellulose…

2017

In the present study, the adsorption of sulfates of sodium sulfate (Na2SO4) and sodium lauryl sulfate (SLS) by calcium hydroxyapatite-modified microfibrillated cellulose was studied in the aqueous solution. The adsorbent was characterized using elemental analysis, Fourier transform infrared, scanning electron microscope and elemental analysis in order to gain the information on its structure and physico-chemical properties. The adsorption studies were conducted in batch mode. The effects of solution pH, contact time, the initial concentration of sulfate and the effect of competing anions were studied on the performance of synthesized adsorbent for sulfate removal. Adsorption kinetics indica…

SodiumInorganic chemistrychemistry.chemical_element02 engineering and technology010501 environmental sciencessulfate removal01 natural sciencesNanocellulosechemistry.chemical_compoundAdsorptionSodium sulfateSpectroscopy Fourier Transform InfraredEnvironmental ChemistryCelluloseSulfateCelluloseWaste Management and Disposalnanocellulose0105 earth and related environmental sciencesWater Science and TechnologyAqueous solutionSulfatesGeneral Medicinewater treatmentHydrogen-Ion Concentration021001 nanoscience & nanotechnologycalcium hydroxyapatiteSolutionsKineticsDurapatitechemistryChemical engineeringElemental analysisadsorptionThermodynamicsAdsorption0210 nano-technologyWater Pollutants ChemicalEnvironmental technology
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Thermoanalytical studies on sodium 1,2-naphthoqoinone oximesolfonates

1991

Abstract Five sodium 1,2-nitrosonaphtholsulfonatea were prepared or purified and their thermal behaviour studied by TG, DTG and DSC. Pyrolysis MS technique was used to identify evolved gases and FTIR to characterize residuals. The water contents depended on preparation and conservation. The decomposition was analysed in three stages which can be formed by one or more steps. The final product was sodium sulfate in air at 530 °C and the mixture of carbon and sodium sulfide at 950 °C in nitrogen. The structure effects on decomposition is discussed.

SodiumInorganic chemistrychemistry.chemical_elementCondensed Matter PhysicsDecompositionNitrogenSodium sulfidechemistry.chemical_compoundchemistrySodium sulfatePhysical and Theoretical ChemistryFourier transform infrared spectroscopyInstrumentationCarbonPyrolysisThermochimica Acta
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CCDC 809075: Experimental Crystal Structure Determination

2011

Related Article: K.Raatikainen, J.Huuskonen, K.Rissanen|2011|Dalton Trans.|40|5706|doi:10.1039/c1dt10406c

Space GroupCrystallography(3639-bis(((5'-Carboxy-22'-bipyridin-5-yl)carbonyl)amino)-42-(((5'-carboxylato-22'-bipyridin-5-yl)carbonyl)amino)-92031-triaza-11223-triazoniaheptacyclo[29.2.2.2^912^.2^2023^.1^37^.1^1418^.1^2529^]dotetraconta-3(42)4614(39)151725(36)2628-nonaene)-iron(ii) disulfate hexacosahydrateCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 1915747: Experimental Crystal Structure Determination

2019

Related Article: L. Tapmeyer, D. Prill|2019|IUCrData|4|x190689|doi:10.1107/S2414314619006898

Space GroupCrystallographyCrystal System(6-amino-5-azaniumyl-4-oxo-34-dihydropyrimidin-2(1H)-ylidene)ammonium sulfate monohydrateCrystal StructureCell ParametersExperimental 3D Coordinates
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CCDC 978468: Experimental Crystal Structure Determination

2014

Related Article: Diana Dragancea, Sergiu Shova, Eva A. Enyedy, Martin Breza, Peter Rapta, Luca M. Carrella, Eva Rentschler, Anatolie Dobrov, Vladimir B. Arion|2014|Polyhedron|80|180|doi:10.1016/j.poly.2014.03.039

Space GroupCrystallographyCrystal System(mu~2~-2-(2-oxidobenzylidene)-N-((2-oxidophenyl)methylidene)hydrazinecarbohydrazonato)-aqua-bis(NN-dimethylformamide)-di-copper(ii) hydrogen sulfate monohydrateCrystal StructureCell ParametersExperimental 3D Coordinates
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