Search results for "FEM"

showing 10 items of 28965 documents

Selective p38α MAP kinase/MAPK14 inhibition in enzymatically modified LDL-stimulated human monocytes: implications for atherosclerosis.

2016

The first ATP-competitive p38α MAPK/MAPK14 inhibitor with excellent in vivo efficacy and selectivity, skepinone-L, is now available. We investigated the impact of selective p38α MAPK/MAPK14 inhibition on enzymatically modified LDL (eLDL) stimulated human monocytes with its implications for atherosclerosis. Among the different p38 MAPK isoforms, p38α/MAPK14 was the predominantly expressed and activated isoform in isolated human peripheral blood monocytes. Moreover, eLDL colocalized with macrophages positive for p38α MAPK/MAPK14 in human carotid endarterectomy specimens. Using the human leukemia cell line THP-1 and/or primary monocyte-derived macrophages, skepinone-L inhibited eLDL-induced ac…

0301 basic medicineAdultMaleChemokineMAP Kinase Signaling Systemp38 mitogen-activated protein kinasesCD36CCL4Dibenzocycloheptenes030204 cardiovascular system & hematologyBiochemistryGene Expression Regulation EnzymologicMonocytesMitogen-Activated Protein Kinase 1403 medical and health sciences0302 clinical medicineCell Line TumorGeneticsHumansInterleukin 8Molecular BiologyFoam cellMAPK14AgedAged 80 and overCaspase 7biologyChemistryCaspase 3Cholesterol LDLAtherosclerosisMolecular biology030104 developmental biologyBiochemistryMitogen-activated protein kinasebiology.proteinFemaleBiotechnologyFASEB journal : official publication of the Federation of American Societies for Experimental Biology
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Postprandial Changes in Chemokines Related to Early Atherosclerotic Processes in Familial Hypercholesterolemic Subjects: A Preliminary Study.

2015

Familial hypercholesterolemia (FH) is associated with higher levels of inflammatory mediators such as chemokines, which contribute to an increased risk of premature atherosclerosis in these patients. We studied the response of chemokines related to early atherosclerotic processes during an oral unsaturated fat load test (OFLT) in patients with heterozygous FH and compared this response to normolipidemic and normoglycemic subjects.Blood samples were taken from 12 FH patients and 20 healthy controls with a similar age, gender distribution, and body mass index. Plasma chemokine levels were determined in both groups in a fasting state and at 2, 4, 6, and 8 h after an OFLT using human cytokine m…

0301 basic medicineAdultMaleChemokinemedicine.medical_specialtyAdolescentmedicine.medical_treatmentFamilial hypercholesterolemia030204 cardiovascular system & hematologyBody Mass IndexHyperlipoproteinemia Type II03 medical and health scienceschemistry.chemical_compoundYoung Adult0302 clinical medicineDietary Fats UnsaturatedInternal medicineMedicineHumansHyperlipoproteinemia Type IIAgedbiologybusiness.industryUnsaturated fatCase-control studyGeneral MedicineFastingMiddle Agedmedicine.diseaseAtherosclerosisPostprandial PeriodHealthy Volunteers030104 developmental biologyEndocrinologyCytokinePostprandialchemistryCase-Control Studiesbiology.proteinFemaleChemokinesbusinessBody mass indexArchives of medical research
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Inferior alveolar nerve trajectory, mental foramen location and incidence of mental nerve anterior loop

2017

Background: Injury of the inferior alveolar nerve (IAN) is a serious intraoperative complication that may occur during routine surgical procedures, such as dental implant placement or extraction of impacted teeth. Thus, the purpose of this study was to analyze the trajectory of the mandibular canal (MC), the location of the mental foramen (MF) and the presence and extension of an anterior loop of the mental nerve (AL). Study Design: In this cross-sectional study, a total of 348 CBCTs were analyzed. Distances from MC to the surface of the basal, medial and lateral cortical of the mandible were measured at the level of the second molar, first molar and second premolar. Location of the MF rela…

0301 basic medicineAdultMaleChinAdolescentMandibular NerveMandibular canalInferior alveolar nerveMandibular first molarMental foramenMandibular second molar03 medical and health sciencesYoung Adult0302 clinical medicinestomatognathic systemPremolarMedicineHumansGeneral DentistryUNESCO:CIENCIAS MÉDICASAgedRetrospective StudiesAged 80 and overbusiness.industryResearchMandible030206 dentistryAnatomyCone-Beam Computed TomographyMiddle AgedMental nervemedicine.anatomical_structureCross-Sectional StudiesOtorhinolaryngologySurgeryFemale030101 anatomy & morphologyOral Surgerybusiness
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Predicting limiting 'free sugar' consumption using an integrated model of health behavior.

2019

Excess intake of 'free sugars' is a key predictor of chronic disease, obesity, and dental ill health. Given the importance of determining modifiable predictors of free sugar-related dietary behaviors, we applied the integrated behavior change model to predict free sugar limiting behaviors. The model includes constructs representing 'reasoned' or deliberative processes that lead to action (e.g., social cognition constructs, intentions), and constructs representing 'non-conscious' or implicit processes (e.g., implicit attitudes, behavioral automaticity) as predictors of behavior. Undergraduate students (N = 205) completed measures of autonomous and controlled motivation, the theory of planned…

0301 basic medicineAdultMaleDietary Sugarsmedia_common.quotation_subjectHealth BehaviorAutomaticity030209 endocrinology & metabolismIntentionStructural equation modeling03 medical and health sciencesYoung Adult0302 clinical medicineSocial cognitionHumansStudentsGeneral Psychologymedia_common030109 nutrition & dieteticsNutrition and DieteticsBehavior changeTheory of planned behaviorBayes TheoremFeeding BehaviorDietTranstheoretical ModelAction (philosophy)FemaleHabitImplicit attitudePsychologyPsychological TheorySocial psychologyAttitude to HealthAppetite
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Rescuing Over-activated Microglia Restores Cognitive Performance in Juvenile Animals of the Dp(16) Mouse Model of Down Syndrome.

2020

Microglia are brain-resident immune cells and regulate mechanisms essential for cognitive functions. Down syndrome (DS), the most frequent cause of genetic intellectual disability, is caused by a supernumerary chromosome 21, containing also genes related to the immune system. In the hippocampus of the Dp(16) mouse model of DS and DS individuals, we found activated microglia, as assessed by their morphology; activation markers; and, for DS mice, electrophysiological profile. Accordingly, we found increased pro-inflammatory cytokine levels and altered interferon signaling in Dp(16) hippocampi. DS mice also showed decreased spine density and activity of hippocampal neurons and hippocampus-depe…

0301 basic medicineAdultMaleDown syndromeDendritic spinemedicine.medical_treatmentAminopyridinesMice TransgenicHippocampal formationHippocampus03 medical and health sciencesMice0302 clinical medicineImmune systemCognitionMedicineHippocampus (mythology)AnimalsHumansPyrrolesNeuroinflammationMicrogliabusiness.industryGeneral NeuroscienceAnti-Inflammatory Agents Non-SteroidalAge Factorsmedicine.disease3. Good healthMice Inbred C57BLDisease Models Animal030104 developmental biologymedicine.anatomical_structureCytokinenervous systemFemaleMicrogliaDown SyndromebusinessNeuroscience030217 neurology & neurosurgeryNeuron
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Reducing diagnostic turnaround times of exome sequencing for families requiring timely diagnoses

2017

IF 2.137; International audience; BACKGROUND AND OBJECTIVE:Whole-exome sequencing (WES) has now entered medical practice with powerful applications in the diagnosis of rare Mendelian disorders. Although the usefulness and cost-effectiveness of WES have been widely demonstrated, it is essential to reduce the diagnostic turnaround time to make WES a first-line procedure. Since 2011, the automation of laboratory procedures and advances in sequencing chemistry have made it possible to carry out diagnostic whole genome sequencing from the blood sample to molecular diagnosis of suspected genetic disorders within 50 h. Taking advantage of these advances, the main objective of the study was to impr…

0301 basic medicineAdultMaleExome sequencingmedicine.medical_specialtyTime FactorsAdolescentGenetic counselingBioinformaticsTurnaround timeSensitivity and SpecificityUndiagnosed genetic conditions03 medical and health sciencesGeneticsmedicineHumansExomeGenetic TestingMedical diagnosisIntensive care medicineChildExomeGenetics (clinical)Exome sequencingGenetic testingWhole genome sequencing[SDV.GEN]Life Sciences [q-bio]/Geneticsmedicine.diagnostic_testbusiness.industryInfant NewbornInfantGeneral MedicineSequence Analysis DNADiagnostic turnaround time3. Good healthClinical trial030104 developmental biologyEarly DiagnosisChild PreschoolFemalebusiness[ SDV.GEN ] Life Sciences [q-bio]/Genetics
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No long-term effect of a 2-days intervention on how to prepare homemade food, on toddlers’ skepticism for new food and intake of fruits and vegetable…

2017

Objective Optimal nutrition from early age reduces the risk of developing non-communicable diseases later in life. The aim of this study was to examine the long-term effect on toddlers’ fruit and vegetable intake and sweet beverages, and skepticism for new food, of a 2-days’ intervention on how to prepare homemade food for toddlers. Results The effect of the cooking intervention was evaluated by a randomized, controlled trial where 110 parents of 4–6 months old infants were included. Child diet and food skepticism were measured at 6, 15 and 24 months of age. There were no differences between the control and intervention group in the consumption of fruits and vegetables and intake of water o…

0301 basic medicineAdultMaleFood skepticismDietary Sugarsmedia_common.quotation_subjectlcsh:MedicineIntervention groupGeneral Biochemistry Genetics and Molecular Biologylaw.inventionBeverages03 medical and health sciencesEatingFood PreferencesRandomized controlled triallawFood intakeIntervention (counseling)Environmental healthVegetablesMedicineHumansTerm effectFood scienceCookinglcsh:Science (General)Trial registrationlcsh:QH301-705.5Skepticismmedia_commonToddlers030109 nutrition & dieteticsbusiness.industrylcsh:Rdigestive oral and skin physiologyInfantGeneral MedicineDietResearch Notelcsh:Biology (General)Optimal nutritionFruits and vegetablesFruitFemaleInfant Foodbusinesslcsh:Q1-390Cooking courseBMC Research Notes
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In vivo muscle morphology comparison in post-stroke survivors using ultrasonography and diffusion tensor imaging.

2019

AbstractSkeletal muscle architecture significantly influences the performance capacity of a muscle. A DTI-based method has been recently considered as a new reference standard to validate measurement of muscle structure in vivo. This study sought to quantify muscle architecture parameters such as fascicle length (FL), pennation angle (PA) and muscle thickness (tm) in post-stroke patients using diffusion tensor imaging (DTI) and to quantitatively compare the differences with 2D ultrasonography (US) and DTI. Muscle fascicles were reconstructed to examine the anatomy of the medial gastrocnemius, posterior soleus and tibialis anterior in seven stroke survivors using US- and DTI-based techniques…

0301 basic medicineAdultMaleFysiologiPhysiologylcsh:MedicineSkeletal musclelihaksettuki- ja liikuntaelimetArticle03 medical and health sciencesMuscle morphology0302 clinical medicinemorfologiaIn vivomedicineHumansin vivo -menetelmädiffuusiotensorikuvausSurvivorsskeletal musclelcsh:ScienceMuscle SkeletalAgedUltrasonographyMultidisciplinarybusiness.industrylcsh:RSkeletal muscleMiddle AgedIndividual leveldiffusion tensor imagingStroke030104 developmental biologymedicine.anatomical_structureDiffusion Tensor ImagingPost strokelcsh:QFemaleUltrasonographyMuscle architecturebusinessBiomedical engineering030217 neurology & neurosurgeryBiomedical engineeringDiffusion MRIScientific reports
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Dynamics of Torque Teno virus plasma DNAemia in allogeneic stem cell transplant recipients

2017

Abstract Background Torque Teno virus (TTV) plasma DNA load directly correlate with the level of immunosuppresion in different clinical settings. It is uncertain whether this may be the case in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). Objectives We characterized the dynamics of TTV DNAemia in patients undergoing T-cell replete allo-SCT. Study design Retrospective single-center observational study including 72 allo-HSCT patients. Plasma TTV DNA loads were quantified before initiating the conditioning regimen and at different time-points after transplant by real-time PCR. White blood cells (WBC) and absolute lymphocyte counts (ALC) were measured by flow cytometry.…

0301 basic medicineAdultMaleHomologousTorque teno virusAdolescentLymphocyteAllogeneic hematopoietic stem cell transplantation (Allo-HSCT); Immune reconstitution; Torque Teno virus (TTV); TTV DNAemia; Adolescent; Adult; Aged; DNA Virus Infections; DNA Viral; Female; Humans; Immunocompromised Host; Leukocyte Count; Male; Middle Aged; Retrospective Studies; Torque teno virus; Transplantation Homologous; Viral Load; Viremia; Young Adult; Hematopoietic Stem Cell Transplantation; Virology; Infectious Diseases030106 microbiologyTTV DNAemiaFlow cytometry03 medical and health sciencesImmunocompromised HostLeukocyte CountYoung AdultVirologyMedicineTransplantation HomologousHumansIn patientViralViremiaProspective cohort studyAgedRetrospective StudiesTorque teno virusTransplantationmedicine.diagnostic_testbusiness.industryHematopoietic Stem Cell TransplantationDNAImmune reconstitutionMiddle AgedViral LoadDNA Virus Infectionssurgical procedures operative030104 developmental biologymedicine.anatomical_structureInfectious DiseasesAllogeneic hematopoietic stem cell transplantation (Allo-HSCT); Immune reconstitution; Torque Teno virus (TTV); TTV DNAemia; Adolescent; Adult; Aged; DNA Virus Infections; DNA; Viral; Female; Humans; Immunocompromised Host; Leukocyte Count; Male; Middle Aged; Retrospective Studies; Torque teno virus; Transplantation; Homologous; Viral Load; Viremia; Young Adult; Hematopoietic Stem Cell Transplantation; Virology; Infectious DiseasesImmunologyDNA ViralAllogeneic hematopoietic stem cell transplantation (Allo-HSCT)FemaleAllogeneic hematopoietic stem cell transplantStem cellImmunocompetencebusinessTorque Teno virus (TTV)
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Thermosensory mapping of skin wetness sensitivity across the body of young males and females at rest and following maximal incremental running

2019

Key points: Humans lack skin receptors for wetness (i.e. hygroreceptors), yet we present a remarkable wetness sensitivity. Afferent inputs from skin cold-sensitive thermoreceptors are key for sensing wetness; yet, it is unknown whether males and females differ in their wetness sensitivity across their body and whether high intensity exercise modulates this sensitivity. We mapped sensitivity to cold, neutral and warm wetness across five body regions and show that females are more sensitive to skin wetness than males, and that this difference is greater for cold than warm wetness sensitivity. We also show that a single bout of maximal exercise reduced the sensitivity to skin wetness (i.e. hyg…

0301 basic medicineAdultMaleHot TemperaturePhysiologyRestSensationPhysiologySensory systemBiologyWetnessRunning03 medical and health sciences0302 clinical medicineCutaneous receptorPhysical StimulationSkin Physiological PhenomenaSensationHumansThermosensingSensitivity (control systems)Skin wetnessExerciseSkinBody surface areaSex CharacteristicsFootThermoreceptorsCold Temperature030104 developmental biologyTouch PerceptionTouchThermoreceptorBody regionFemaleSexSkin Temperature030217 neurology & neurosurgeryBody Temperature Regulation
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