Search results for "FLUORESCENCE MICROSCOPY"
showing 10 items of 61 documents
Molecular Insights on alpha-synuclein mediated membrane disruption: protein-lipid co-aggregates formation
2014
In Toto Cell Tracking using Lightsheet Microscopy of the Neuromesodermal Progenitor Zone of the Zebrafish Tailbud from the 21st Somite Stage
2018
Lightsheet microscopy has been previously used to image and directly study the process of gastrulation in a zebrafish embryo. Before now it was not possible to use this method to study late-stage somatogenesis, due to the movement of the embryo, resulting in displacement from the field of view. Here we achieve 4D lightsheet imaging with online, image based registration to maintain the field of view during long timelapses, with emphasis on the tailbud. The zebrafish tailbud is of special interest as it contains a population of bipotent progenitor cells, neuromesodermal progenitors (NMps), competent to enter either the mesodermal or neural lineages. This dataset provides in toto tracking of t…
Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System
2021
Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants …
Immunological and ultrastructural characterization of spirotrichonymphid flagellates from Reticulitermes grassei and R. flavipes (syn. R. santonensis…
2006
AbstractFive species of spirotrichonymphids representing three genera have been studied by light and immunofluorescence microscopy, and by transmission electron microscopy. The genus Spirotrichonympha, represented by S. flagellata from Reticulitermes grassei, is characterized by a compound axostyle composed of several fibers or subaxostyles. The genus Spironympha, represented by S. kofoidi from Reticulitermes flavipes (syn. R. santonensis) and by the two new species S. verticis and S. lanceata, is characterized by flagellar lines restricted to the anterior area and a simple, tubular axostyle. Spironympha verticis and S. lanceata are mainly distinguished by ultrastructural details of their f…
Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton
2021
The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytometry in assessing the composition, abundance and cell sizes of autofluorescent picophytoplankton in epilimnia of 46 Finnish lakes. Phycocyaninrich picocyanobacteria were the most dominant. The two methods yielded comparable total picophytoplankton abundances, but the determination of cell sizes, and thus total biomasses, were on average an order of magnitude higher in the microscopy results. Howev…
In vivo analysis of noise dependent activation of white blood cells and microvascular dysfunction in mice
2021
This article contains supporting information on data collection for the research article entitled “Aircraft noise exposure drives the activation of white blood cells and induces microvascular dysfunction in mice” by Eckrich et al. We found that noise-induced stress triggered microvascular dysfunction via involvement of innate immune-derived reactive oxygen species. In this article, we present the instrumentation of mice with dorsal skinfold chambers for in vivo microscopic imaging of blood flow, interaction of leukocytes with the vascular wall (also by fluorescent labelling of blood cells) and vessel diameter. In addition, we explain the preparation of cerebral arterioles for measurement of…
Algorithms and software for biological multiscale image analysis
2013
Influence of Nanoparticle Exposure on Nervous System Development in Zebrafish Studied by Means of Light Sheet Fluorescence Microscopy
2016
Zebrafish has a remarkable similarity in the molecular signaling processes, cellular structure, anatomy and physiology to other higher order vertebrates, making it an excellent vertebrate model organism (1). Recently, zebrafish has been used for neurotoxicity screening of numerous nanomaterials with a focus on the developmental effects due to the possibility of in vivo visualization of specific neurons and axon tracts by injecting dyes in live animals as well in fixed ones (2). Here we propose Light Sheet Fluorescence Microscopy (LSFM) (3) (4) to perform neurotoxicity studies, in order to study the nervous system architecture and to image 3D structures in the brain of live larvae during the…
A Novel Fast Volumetric Light Sheet Microscopy
2016
Fast noninvasive three-dimensional (3D) imaging is crucial for the quantitative understanding of highly dynamic biological processes. Over the last decades, several fluorescence microscopy techniques have been developed in order to provide a faster and deeper imaging of thick biological samples [1]. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) has emerged as a powerful imaging tool for 3D imaging of thick samples ranging from single cells to entire animals [2,3].However, to obtain a 3D reconstruction either sample or microscope parts usually need to be moved limiting the acquisition speed and inducing possible interferences in volume recording. To solve this problem, he…
Spectroscopic studies of semiconducting single-walled carbon nanotubes
2010
The unique nature of optical properties of single-walled carbon nanotubes (SWCNT), together with their promising potential applications, have created enormous interest towards the photophysics of SWCNT. Many aspects of carbon nanotubes originate from the electronic structure of carbon honeycomb lattice and one-dimensionality. SWCNTs exist in various chiral structures and diameters, which the optical and electrical properties are dependent on. It has been discovered that SWCNT excited states are excitonic with strong Coulomb interaction between the electron and the hole. However, many features of excitons are not yet well defined, such as absorption cross-sections, fluorescence quantum yield…