Search results for "FORMS"
showing 10 items of 668 documents
Simultaneous dissolution profiles of two drugs, sulfadiazine-trimethoprim and amitriptyline-perphenazine, in solid oral dosage forms by a FIA manifol…
2002
The simultaneous determination of two dissolution profiles with the aid of a Flow Injection Analysis assembly has been applied to: (a) sulfadiazine-trimethoprim in tablets and (b) amitriptyline-perphenazine in sugar coated pills. The selected combinations are drugs which have overlapping UV-vis spectra. The officially proposed procedure from the pharmacopoeias has been adapted for the FIA methodology and derivative spectrophotometry and zero crossing. Preliminary experiments on the suitability of the simultaneous determination of both drugs were performed. The empirical profiles were adjusted by regression analysis using different approaches. The 3-parameter plot method was finally selected…
Usher syndrome: molecular links of pathogenesis, proteins and pathways.
2006
Contains fulltext : 50437.pdf (Publisher’s version ) (Closed access) Usher syndrome is the most common form of deaf-blindness. The syndrome is both clinically and genetically heterogeneous, and to date, eight causative genes have been identified. The proteins encoded by these genes are part of a dynamic protein complex that is present in hair cells of the inner ear and in photoreceptor cells of the retina. The localization of the Usher proteins and the phenotype in animal models indicate that the Usher protein complex is essential in the morphogenesis of the stereocilia bundle in hair cells and in the calycal processes of photoreceptor cells. In addition, the Usher proteins are important in…
Isolation and characterization of two T-box genes from sponges, the phylogenetically oldest metazoan taxon
2003
It is now well established that all metazoan phyla derived from one common ancestor, the hypothetical Urmetazoa. Due to the basal position of Porifera (Demospongiae) in the phylogenetic tree of Metazoa, studies on the mechanisms controlling the development of these animals can provide clues on the understanding of the origin of multicellular animals and on how the first organization of the body plan evolved. In this report we describe the isolation and genomic characterization of two T-box genes from the siliceous sponge Suberites domuncula. The phylogenetic analysis classifies one into the subfamily of Brachyury, Sd-Bra, and the second into the Tbx2 subfamily, Sd-Tbx2. Analyses of the Sd-B…
Usher syndrome and Leber congenital amaurosis are molecularly linked via a novel isoform of the centrosomal ninein-like protein.
2009
Contains fulltext : 80984.pdf (Publisher’s version ) (Closed access) Usher syndrome (USH) and Leber congenital amaurosis (LCA) are autosomal recessive disorders resulting in syndromic and non-syndromic forms of blindness. In order to gain insight into the pathogenic mechanisms underlying retinal degeneration, we searched for interacting proteins of USH2A isoform B (USH2A(isoB)) and the LCA5-encoded protein lebercilin. We identified a novel isoform of the centrosomal ninein-like protein, hereby named Nlp isoform B (Nlp(isoB)), as a common interactor. Although we identified the capacity of this protein to bind calcium with one of its three EF-hand domains, the interacton with USH2A(isoB) did …
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
2009
We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flanking a given splice junction allows to specifically quantify, within the cell, the RNA isoform generating such splice junction by FRET measure. As a proof of concept we analyzed two alternative splicing events originating from lymphocyte antigen 6 (LY6) complex, locus G5B (LY6G5B) pre-mRNA. These are characterized by the removal of the first intron (Fully Spliced Isoform, FSI) or by retention of suc…
Digital Platforms for Restructuring the Public Sector
2018
Many technological innovations have led to the emergence of the platform economy in recent years. This development is changing the entire landscape of business in the era of digitalisation. However, the impacts of the platform economy on public services and government are not well known. In this article we study the potential for the digital platform economy to help restructure the public sector. Firstly, central features of the new platform technology are explored, pointing to an algorithmic revolution, big data and cloud computing. Platforms are used in coordinating market transactions in an extremely efficient way. In order to apply the platform-concept to the public sector, an experimen…
Functional linear models for the analysis of similarity of waveforms
2018
In seismology methods based on waveform similarity analysis are adopted to identify sequences of events characterized by similar fault mechanism and prop- agation pattern. Seismic waves can be considered as spatially interdependent three dimensional curves depending on time and the waveform similarity analysis can be configured as a functional clustering approach, on the basis of which the member- ship is assessed by the shape of the temporal patterns. For providing qualitative ex- traction of the most important information from the recorded signals we propose an integration of the metadata, related to the waves, as explicative variables of a func- tional linear models. The temporal pattern…
Historia y evolución del acogimiento familiar de menores y el contexto de la Comunidad Valenciana
2012
En este articulo presentamos la historia y evolución del Sistema de Protección a la Infancia, y más concretamente el recurso del acogimiento familiar de menores, a lo largo de los últimos veinticinco años. En el mismo diferenciamos dos partes: La primera, referida a la génesis del marco legislativo -internacional y estatal-, y la concreción de las modalidades de acogimiento en familia ajena y extensa. En la segunda parte se desarrolla el transcurso del acogimiento familiar de menores en el contexto de la Comunidad Valenciana. This article presents the history and evolution undergone over the last twenty-five years by the System for Minor Protection, and more specifically the solution of fos…
Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.
2004
Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, Gt, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca(2+)-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to Gtbetagamma heterodimers. High affinity b…
Short forms of the Wechsler adult scales: a systematic review
2020
espanolIntroduccion.Desde la aparicion de la primera escala WAIS, ha habido un intento generalizado por parte de investigadores/as en la evaluacion de las habilidades cognitivas por encontrar formas abreviadas que faciliten la practica clinica de los/as profesionales en aquellas situaciones en las que las condiciones, bien por el tiempo disponible o por las caracteristicas clinicas de los/as pacientes, no aconsejan utilizar un ins-trumento extenso. Objetivo.Llevar a cabo una revision sistematica de las formas breves de hasta cuatro subtests elaboradas a partir de las escalas WAIS-III y WAIS-IV. Metodologia.Busqueda de informacion en la base de datos PsycINFO y revision sistematica de la inf…