Search results for "Fertilisation"

Dynamique de la communauté microbienne de sols tropicaux suite à une application répétée de fertilisants organiques et minéraux

2019

Organic waste products (OWP) with NPK mineral fertilization are known to have an impact on soil microbial communities. However, dynamic of these communities following a repeated OWP and NPK application has not been well described. In the present study, this dynamic was observed on soil sampled at the La Mare (La Réunion) field experiment, which was set up in 2013. This experiment includes control plot (without organic input, but mineral fertilization) and several plots receiving different types of OWP coupled with NPK fertilization. Physico-chemical parameters were measured on soils and OWPs (2013 to 2017). Soil microbial biomass (from 2013 to 2018) and the structure of microbial community …

APOPTOTIC ANALYSIS OF CUMULUS CELLS FOR THE SELECTION OF COMPETENT OOCYTES TO BE FERTILIZED BY INTRACYTOPLASMIC SPERM INJECTION (ICSI)

2015

Oocyte quality is one of the main factors for the success of in vitro fertilization protocols. Apoptosis is known to affect oocyte quality and may impair subsequent embryonic development and implantation. The aim of this study was to investigate the apoptosis rate of single and pooled cumulus cells of cumulus cell–oocyte complexes (COCs), as markers of oocyte quality, prior to intracytoplasmatic sperm injection (ICSI).We investigated the apoptosis rate by TUNEL assay (DNA fragmentation) and caspase-3 immunoassay of single and pooled cumulus cells of COCs. The results showed that DNA fragmentation in cumulus cells was remarkably lower in patients who achieved a pregnancy than in those who di…

Concentrations of ovarian proteohormones as biological markers for the outcome of assisted reproductive technology

2002

Objective: To assess whether the ovarian proteohormones inhibin A and B, pro-alpha-C and activin A could act as direct biological markers of a poorer or better response to ovulation induction or clinical conception through assisted reproductive technology (ART). Methods: Seventy-seven women who underwent ART were included in the study and were divided into two groups: (i) 46 women with sonographically documented follicular maturation; and (ii) 36 women who demonstrated inadequate follicle development. Blood samples were analyzed for inhibin A, inhibin B, pro-alpha-C, activin A and estradiol (E2) at day 3 of stimulation with recombinant follicle-stimulating hormone (rFSH) after pituitary dow…

L’ammissibilità ratione personae dei ricorsi alla Corte europea di individui estremamente vulnerabili: uguaglianza sostanziale e tutela del diritto d…

2021

The legal framework governing proceedings before the ECtHR does not deal with cases in which victims of human rights violations are not de jure and/or de facto capable of lodging applications with the Court. In the effort of overcoming such a procedural lacuna, the Court has established a test that, taking into consideration the extreme vulnerability of the victims (such as minors and persons with disabilities) and the seriousness of the alleged violations, allows NGOs to apply as their ‘de facto representatives’, provided that some other conditions are fulfilled. The Court’s approach, however, has not been always consistent and does not guarantee predictability and legal certainty. Moreove…

Dynamic of Components Leachate from Experimental Fertilizers in Leaching Test

2018

Organic matter contained in the waste of food industry or occurring in the households, in the absence of contamination with other kind of waste, may be used to create a “fertiliser”, which could be even reused in the organic production of arable crops. Particular attention is drawn to the fertilisers which may be applied in the case of amateur cultivation of pot plants and in the allotment gardens. The paper presents the results of research regarding the dynamics of the release of nutrients from tablets created with the help of encapsulation of the mixture manufactured from solvent extraction waste of coffee (SCG), modified by the ash obtained from low temperature burning of biomass. In thi…

In vitro fertilization in underweight women: focus on obstetric outcome.

2020

Anxiety and depressive symptoms, and stress biomarkers in pregnant women after in vitro fertilization: a prospective cohort study.

2017

STUDY QUESTION Does in vitro fertilization (IVF) affect the course of anxiety and depressive symptoms as well as physiological stress from pregnancy to postpartum period? SUMMARY ANSWER IVF mothers have more anxiety symptoms and higher stress biomarker levels but fewer depression symptoms than natural conception mothers at the third trimester of pregnancy, but these differences are negligible during postpartum period. WHAT IS KNOWN ALREADY Cross-sectional studies have found an association between IVF and high stress levels during the prenatal period. There is, however, no follow-up study about the IVF effect on the mental health status from pregnancy to postpartum, adopting simultaneous mea…

Predictors of live birth in women with a history of biochemical pregnancies after assisted reproduction treatment

2020

Abstract Objectives To ascertain whether women with a history of biochemical pregnancies (BPs) in in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment cycles have decreased chances of live birth (LB); and (2) to build a predictive model for LB in this particular population of infertile women. Methods In order to achieve the first objective, data from 1536 women that had a LB using autologous fresh or frozen embryos, or dropped out of further IVF/ICSI treatments after completing one to three unsuccessful treatment cycles were retrospectively analyzed. A subpopulation of 90 women that experienced one or more BPs in our assisted reproduction unit were selected to buil…

Soluble ligands and their receptors in human embryo development and implantation.

2014

Extensive evidence suggests that soluble ligands and their receptors mediate human preimplantation embryo development and implantation. Progress in this complex area has been ongoing since the 1980s, with an ever-increasing list of candidates. This article specifically reviews evidence of soluble ligands and their receptors in the human preimplantation stage embryo and female reproductive tract. The focus will be on candidates produced by the human preimplantation embryo and those eliciting developmental responses in vitro, as well as endometrial factors related to implantation and receptivity. Pathways to clinical translation, including innovative diagnostics and other technologies, are al…

Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequenti…

2008

Objective To compare embryologic and clinical outcomes in terms of preimplantation development, implantation, pregnancy rates, and secretome profile of implanted blastocysts from the preimplantation genetic diagnosis program grown in sequential versus endometrial epithelial cell (EEC) coculture system. Design Retrospective clinical study and prospective experimental study. Setting In vitro fertilization clinical unit and university research laboratory. Intervention(s) Blastomere biopsy, embryo culture, blastocyst transfer, and protein analysis of the media conditioned from implanted embryos obtained from coculture and sequential systems. Main Outcome Measure(s) Clinical study: blastocyst, i…