Search results for "Fibroin"

Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells

2009

For successful bone regeneration tissue engineered bone constructs combining both aspects, namely a high osteogenic potential and a rapid connection to the vascular network are needed. In this study we assessed the formation of pre-vascular structures by human outgrowth endothelial cells (OEC) from progenitors in the peripheral blood and the osteogenic differentiation of primary human osteoblasts (pOB) on micrometric silk fibroin scaffolds. The rational was to gain more insight into the dynamic processes involved in the differentiation and functionality of both cell types depending on culture time in vitro. Vascular tube formation by OEC was assessed quantitatively at one and 4 weeks of cul…

Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering.

2003

We have examined a novel biomaterial consisting of a non-woven fibroin net produced from silk (Bombyx mori) cocoons for its ability to support the growth of human cells. Various human cells of different tissue and cell types (endothelial, epithelial, fibroblast, glial, keratinocyte, osteoblast) were examined for adherence and growth on the nets by confocal laser microscopy after staining of the cells with calcein-AM and by electron microscopy. All the cells readily adhered and spread over the individual fibers of the nets. Most of the cells were able to grow and survive on the nets for at least 7 weeks and growth not only covered the individual fibers of the net but generally bridged the ga…

Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells

2013

The use of three-dimensional (3D) cultures may induce cardiac progenitor cells to synthesize their own extracellular matrix (ECM) and sarcomeric proteins to initiate cardiac differentiation. 3D cultures grown on synthetic scaffolds may favour the implantation and survival of stem cells for cell therapy when pharmacological therapies are not efficient in curing cardiovascular diseases and when organ transplantation remains the only treatment able to rescue the patient's life. Silk fibroin-based scaffolds may be used to increase cell affinity to biomaterials and may be chemically modified to improve cell adhesion. In the present study, porous, partially orientated and electrospun nanometric n…

Composizione per uso nella finitura, conservazione, restauro di manufatti

2017

L’invenzione è relativa ad una composizione per il consolidamento e la conservazione di fibre tessili basata sull’impiego di un nano materiale in associazione con un agente di supporto. Il nano materiale è la nano fibroina e il materiale di supporto è preferenzialmente il chitosano, ma si possono impiegare altri materiali di analoghe prestazioni. La composizione trova applicazione nell’ambito della conservazione e restauro di materiali tessili, in quanto permette la riparazione di fibre e tessuti danneggiati, ripristinando le proprietà meccaniche, e la protezione da processi foto-ossidativi dei manufatti tessili in generale. Ulteriore campo di applicazione è quello del finissaggio di fibre …

Modified Silk with Cell-Adhesive and Non-Thrombogenic Properties as a Tissue Engineering Substrate

2016

IntroductionReplacement of damaged tissue is nowadays an aim of tissue engineering. This technique involves the use of porous or fibrous structures – the so-called scaffolds – that support the colonization with the desired cell type and which are degraded after fulfilling their temporary supporting function. Basic requirements for the prevailing materials used in this field are nontoxicity, low immunogenicity and cell-adhesiveness. Furthermore blood-contacting devices should exhibit low thrombogenicity.The biopolymer silk, mainly consisiting of the protein silk fibroin, matches some of these criteria but bare silk does not facilitate cellular adhesion and growth and unfortunately the materi…

Silk-based matrices and c-Kit positive cardiac progenitor cells for a cellularized silk fibroin scaffold: study of an in vivo model.

2022

The production of a cellularized silk fibroin scaffold is very difficult because it is actually impossible to differentiate cells into a well-organized cardiac tissue. Without vascularization, not only do cell masses fail to grow, but they may also exhibit an area of necrosis, indicating a lack of oxygen and nutrients. In the present study, we used the so-called tyrosine protein kinase kit (c-kit)-positive cardiac progenitor cells (CPCs) to generate cardiac cellularized silk fibroin scaffolds, multipotent cells isolated from the adult heart to date that can show some degree of differentiation toward the cardiac phenotype. To test their ability to differentiate into the cardiac phenotype in …

Interpenetrating composite biomaterials of hyaluronic acid derivatives and silk fibroin

2014

Functionality of endothelial cells on silk fibroin nets: Comparative study of micro- and nanometric fibre size

2007

Biomimetic material design, such as mimicking nanostructured components of the extracellular matrix, is an actual challenge for biomaterial research with a high impact on tissue engineering and regenerative medicine. Thus, understanding the cellular response at the cell biological and molecular level and the consequences of various chemically or physically modified biomaterials is highly important. In the present study we assessed the response of human umbilical vein endothelial cells (HUVEC) and outgrowth endothelial cells (OEC) from endothelial progenitor cells to different variants of nanofibrous silk fibroin nets in comparison to microfibrous silk fibroin scaffolds with regard to cellul…

Outgrowth endothelial cells isolated and expanded from human peripheral blood progenitor cells as a potential source of autologous cells for endothel…

2006

One challenge of particular importance in tissue engineering is to improve vascularization of larger size defects, which would then facilitate a sufficient supply with oxygen and nutrients to the central regions of a larger tissue-engineered construct or in highly vascularized tissues. In this study, we show that outgrowth endothelial cells (OECs) derived from human peripheral blood can serve as a source of human autologous endothelial cells and can be used in combination with fibroin silk fiber meshes for applications in tissue engineering. OEC reveal a highly differentiated endothelial phenotype as well as a high phenotypic stability during their expansion. Furthermore, OEC showed very pr…

Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of human endothelial cells

2004

Abstract We have previously shown that a biomaterial consisting of a non-woven fibroin net produced from silk (Bombyx mori) cocoons is an excellent scaffolding material for a wide variety of human cells of different tissue types. Endothelialization must take place for a biomaterial to be successful after implantation. Therefore, primary human endothelial cells and the human endothelial cell lines, HPMEC-ST1.6R and ISO-HAS-1, were examined for adherence and growth patterns on the fibroin nets by confocal laser scanning microscopy after vital staining of the cells and by electron microscopy. Endothelial cells adhered and spread along individual fibers of the nets and did not fill the gaps bet…