Search results for "Ficus"

Low incidence of Vibrio vulnificus among Vibrio isolates from sea water and shellfish of the western Mediterranean coast.

1999

A specific search for Vibrio vulnificus in natural marine samples from the Spanish Mediterranean Sea was carried out by nested PCR and cultural approaches using thiosulphate-citrate-bile salts-sucrose agar (TCBS) and cellobiose-polymixin B-colistin agar (CPC), incubated at 40 degrees C, as selective media. Presumptive colonies were identified by PCR using specific primers against 23S rRNA sequences. This species was isolated from sea water and edible bivalves, mainly after preenrichment in alkaline peptone water (APW) at 40 degrees C followed by CPC agar. None of the V. vulnificus isolates identified corresponded to serovar E. Dominant Vibrio species on directly inoculated TCBS plates incub…

Vibrio vulnificus produces quorum sensing signals of the AHL-class

2009

Vibrio vulnificus is an aquatic pathogenic bacterium that can cause vibriosis in humans and fish. The species is subdivided into three biotypes with the fish-virulent strains belonging to biotype 2. The quorum sensing (QS) phenomenon mediated by furanosyl borate diester or autoinducer 2 (AI-2) has been described in human strains of biotype 1, and here we show that the luxS gene which encodes AI-2 is present in all strains of V. vulnificus regardless of origin, biotype or serovar. In this study, we also demonstrate that V. vulnificus produces QS signals of the acylated homoserine lactone (AHL) class (AI-1). AHLs were detected in strains of biotype 1 and 2 from water, fish and human wound inf…

Cactus pear (O. ficus-indica (L.) Mill.) fruit production: Ecophysiology, orchard and fresh-cut fruit management

2015

Cactus pear (Opuntia ficus-indica L. Mill.) is cultivated in wide range of environments with the consequence of large differences in crop potential, orchard systems and management. These differences may be related to temperature and rainfall range (water availability) but also to the day/night length and, of course, to soil characteristics. This leads to considerable variability in the field and in fruit quality. Crop value much depends in crop variability in terms of fruit size, which is the major factor for fruit price in Europe. The increase of crop value very much depends on regular cultural practices such as irrigation and fruit thinning, but it is also dependent on plant crop yield an…

An enzyme-linked immunosorbent assay for detection of Vibrio vulnificus biotype 2: development and field studies.

1997

Vibrio vulnificus biotype 2 is a primary eel pathogen which constitutes a lipopolysaccharide (LPS)-based homogeneous O serogroup within the species. In the present work, we have developed an enzyme-linked immunosorbent assay (ELISA) based on the specificity of LPS for the detection of this pathogen. The ELISA specificity was confirmed after testing 36 biotype 2 strains from laboratory cultures and environmental samples, 31 clinical and environmental biotype 1 isolates, and several strains of Vibrio, Aeromonas, and Yersinia species, including the fish pathogens V. anguillarum, V. furnissii, A. hydrophila, and Y. ruckerii. The detection limits for biotype 2 cells were around 10(4) to 10(5) ce…

Enzyme-linked immunosorbent assay for detection of Aeromonas hydrophila serogroup O:19

2006

An enzyme-linked immunosorbent assay has been developed for the detection of Aeromonas hydrophila serogroup O:19 isolated from epizootics in eels. The enzyme-linked immunosorbent assay specificity was confirmed after testing A. hydrophila O:19 and non-O:19 strains from different origins, as well as other Aeromonas species and other fish pathogens such as Vibrio vulnificus biotype 2, V. furnisii, V. damsela, Yersinia ruckerii and Edwardsiella tarda. The detection limits for A. hydrophila O:19 cells were around 10(4)-10(5) cells/well. Artificially infected eels were analyzed and the immunodetection was confirmed by cultural methods. With this methodology A. hydrophila O:19 was successfully de…

Il Ficus di Villa Garibaldi

2011

Il Ficus di Villa Garibaldi a Palermo, emblema vivente dell’Unità d’Italia

2011

Notizie botaniche sul Ficus macrophylla

2011

First record of Vibrio vulnificus biotype 2 from diseased European eel, Anguilla anguilla L.

1991

Fragmentation and local disturbance of forests reduce frugivore diversity and fruit removal in Ficus thonningii trees

2008

Clearance, fragmentation, and degradation of tropical forests have resulted in declines of biodiversity. This loss of biodiversity is endangering important ecosystem processes such as seed dispersal. If anthropogenic disturbances affect seed dispersal of keystone plants, effects on tropical ecosystems might be especially pronounced. We studied frugivore assemblages and fruit removal from 25 Ficus thonningii trees in the heavily fragmented and disturbed Kakamega Forest, western Kenya. During 400 observation hours we recorded 36 frugivores visiting F. thonningii trees. We recorded significantly fewer frugivorous species in fragments compared to the main forest and in highly, compared to littl…