Search results for "Ficus"
showing 10 items of 211 documents
Early steps in the European eel (Anguilla anguilla)–Vibrio vulnificus interaction in the gills: Role of the RtxA13 toxin
2015
Vibrio vulnificus is an aquatic gram-negative bacterium that causes a systemic disease in eels called warm-water vibriosis. Natural disease occurs via water born infection; bacteria attach to the gills (the main portal of entry) and spread to the internal organs through the bloodstream, provoking host death by haemorrhagic septicaemia. V.vulnificus produces a toxin called RtxA13 that hypothetically interferes with the eel immune system facilitating bacterial invasion and subsequent death by septic shock. The aim of this work was to study the early steps of warm-water vibriosis by analysing the expression of three marker mRNA transcripts related to pathogen recognition (tlr2 and tlr5) and in…
Transmission to Eels, Portals of Entry, and Putative Reservoirs of Vibrio vulnificus Serovar E (Biotype 2)
2001
ABSTRACT Vibrio vulnificus serovar E (formerly biotype 2) is the etiologic agent that is responsible for the main infectious disease affecting farmed eels. Although the pathogen can theoretically use water as a vehicle for disease transmission, it has not been isolated from tank water during epizootics to date. In this work, the mode of transmission of the disease to healthy eels, the portals of entry of the pathogen into fish, and their putative reservoirs have been investigated by means of laboratory and field experiments. Results of the experiments of direct and indirect host-to-host transmission, patch contact challenges, and oral-anal intubations suggest that water is the prime vehicle…
Isolation of a hemin and hemoglobin binding outer membrane protein of Vibrio vulnificus biotype 2 (serogroup E)
2006
The eel pathogen Vibrio vulnificus biotype 2 (serogroup E) is able to use hemin (Hm) or hemoglobin (Hb) as the sole iron source for growth in vitro and in vivo. The mechanism of heme-iron acquisition in this bacterium requires a direct interaction through binding sites on the bacterial surface (constitutive outer membrane proteins). Using affinity chromatography techniques, a unique protein of around 36.5 kDa was isolated from cell envelopes of E86 strain regardless of the affinity ligand used, hemoglobin or hemin. This protein was purified from both iron-enriched and iron-restricted grown cells. These results support the hypothesis that in this pathogen Hm- and Hb-iron acquisition is media…
Increasing Cold Tolerance of Cactus Pear Fruit by High-Temperature Conditioning and Film Wrapping
2017
Pre-storage high-temperature conditioning (HTC, 38 °C, and 95% RH for 24 h) and individual film wrapping (IFW) with a perforated polyolefinic heat-shrinkable film were used as individual treatments or in combination to mitigate chilling injury of first crop cactus pear cv âGiallaâ. The fruit was stored for 21 days at either 2 or 8 °C (CS) plus 1 week of simulated marketing conditions (SMC) at 20 °C. The reduction in peel disorders and decay in HTC-treated fruit stored at 2 °C was comparable to that detected in control fruit stored at 8 °C. IFW was more efficient than HTC in reducing peel disorders, almost completely inhibited weight loss, and preserved freshness in fruit st…
Quality of cactus pear [Opuntia ficus-indica (L.) Mill.] fruit in relation to ripening time, CaCl2 pre-harvest sprays and storage conditions
1999
Abstract Post-harvest behaviour of summer and autumn ripening cactus pear ( Opuntia ficus-indica (L.) Mill. Cactaceae) fruit cv. Gialla was investigated in relation to pre-harvest growing conditions, and CaCl 2 applications. Summer ripening cactus pear fruit were stored for 5 weeks at 6°C and 90–95% relative humidity (RH), and 3 days at 20°C, 75% RH to simulate a marketing period (SMP). Autumn ripening fruits were kept under the same conditions for 7 weeks plus a SMP. Summer ripening cactus pear fruit were more susceptible to chilling injury and to weight loss, but less sensitive to decay than autumn fruit. CaCl 2 delayed rind colour development and increased fruit resistance to decay but p…
Effect of cladode shading on growth and ripening of fruits of cactus pear (Opuntia ficus-indicaL. Miller)
1997
SummaryFruiting cladodes of O. ficus-indica were covered with shade nets at different stages of the fruit development period to study the effect of the time and duration of shade on fruit growth, ripening time and harvest quality. Shading the fruiting cladodes 0, 15 and 30 d after full bloom (DAB) for a period of 15, 30, 45, 60 or 75 d significantly reduced fruit growth and ultimate harvest size. The longer the shading period the greater was the reduction of harvest fruit size. The greatest reduction of fruit growth (30%) occurred when the fruiting cladodes were shaded from bloom to harvest. Differences in growth rate beween fruits on shaded or sunlit cladodes begun to appear at 30 DAB and …
Toxic and enzymatic activities of Vibrio vulnificus biotype 2 with respect to host specificity
1996
In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed high-level hydrolytic activities, (ii) displayed cytotoxicity for fish cell lines, and (iii) were lethal for eels. Exotoxins seem to be proteinaceous since heat treatment of ECP samples destroyed their toxicity. Only biotype 2 strains were virulent for cels, suggesting that host specificity must be related to differences in cell surface properties. Infectivity trials with other…
High Temperatures Result in Smaller Nurseries which Lower Reproduction of Pollinators and Parasites in a Brood Site Pollination Mutualism
2014
In a nursery pollination mutualism, we asked whether environmental factors affected reproduction of mutualistic pollinators, non-mutualistic parasites and seed production via seasonal changes in plant traits such as inflorescence size and within-tree reproductive phenology. We examined seasonal variation in reproduction in Ficus racemosa community members that utilise enclosed inflorescences called syconia as nurseries. Temperature, relative humidity and rainfall defined four seasons: winter; hot days, cold nights; summer and wet seasons. Syconium volumes were highest in winter and lowest in summer, and affected syconium contents positively across all seasons. Greater transpiration from the…
Fresh-cut products from cactus species
2005
Various species of Cactaceae family can serve a source of delicious and healthy fresh foodstuffs. Marketing cacti as ready-to-eat (fresh-cut) products may significantly expand their consumption, on condition that their quality, safety and longevity would answer the market demands. The present work was aimed at studying the storability of fresh-cut products prepared from several edible cactus species and looking for ways to improve their keeping quality. The experiments included fruit of cactus pear Opuntia ficus-indica (L.) Miller, cactus apple (koubo) Cereus peruvianus (L.) Miller, and dragon fruit (pitaya) Hylocereus undatus (Haw.) Britton and Rose, as well as cladodes (pads) of nopal (ve…
Host–pathogen interactions in Vibrio vulnificus: responses of monocytes and vascular endothelial cells to live bacteria
2015
ABSTRACT Aim: To demonstrate that Vibrio vulnificus, a sepsis-related aquatic pathogen, can provoke a strong pro-inflammatory reaction in blood-associated target cells. Materials & methods: We selected two strains of the two main phylogenetic lineages, two human cell lines, monocytes and vascular endothelial cells and designed an in vitro infection model simulating early septicemia. Results: Both strains caused a strong cell-specific pro-inflammatory response and produced a high degree of cell damage that ended with death by lysis (endothelial cells) or apoptosis/lysis (monocytes). The interaction with endothelial cells was stronger than expected and significantly different for both l…