Search results for "Filipin"

Plasma membrane sterol complexation, generated by filipin, triggers signaling responses in tobacco cells

2010

International audience; The effects of changes in plasma membrane (PM) sterol lateral organization and availability on the control of signaling pathways have been reported in various animal systems, but rarely assessed in plant cells. In the present study, the pentaene macrolide antibiotic filipin III, commonly used in animal systems as a sterol sequestrating agent, was applied to tobacco cells. We show that filipin can be used at a non-lethal concentration that still allows an homogeneous labeling of the plasma membrane and the formation of filipin-sterol complexes at the ultrastructural level. This filipin concentration triggers a rapid and transient NADPH oxidase-dependent production of …

El ?Alivio de las Indias?. la Real Hacienda Filipina, 1565-1800

2019

Previo al estudio de la Real Hacienda filipina, el trabajo se centrará en la definición del marco territorial, la función del archipiélago en la estrategia imperial y la gran transformación de finales del siglo xVIII que afectó a su situación en el conjunto colonial. En segundo lugar, se expondrá el estado de la cuestión y las principales hipótesis sobre la evolución del Tesoro filipino y los problemas que planteaba su viabilidad, así como las fuentes y la metodología empleadas. A continuación se examinarán las grandes tendencias de la Hacienda en el largo plazo y los componentes del ingreso y el gasto, para finalizar con algunas conclusiones, en especial la respuesta a la siguiente pregunt…

Intramembrane particles and filipin labelling on the membranes of autophagic vacuoles and lysosomes in mouse liver

1989

Morphologically detectable protein (intramembrane particles) and cholesterol (filipin labelling) in the membranes of autophagic vacuoles and lysosomes were studied in mouse hepatocytes using thin-section and freeze-fracture electron microscopy. Both isolated autophagic vacuoles and lysosomes, and intact tissue blocks were used due to the facts (i) that lysosomes are difficult to recognize in freeze-fracture replicas of intact hepatocytes, and (i) that filipin penetration into the tissue blocks is unsatisfactory. Intramembrane particle density was low in the membranes of early autophagic vacuoles (defined as round-shaped vacuoles in which an inner membrane parallel with the outer limiting me…

Mordaza de gerga, a blasfemias de paño

Signat ala fi per Fr. Iuan Cortès Ossorio, aunque Uriarte indica que no es obra seva Sign.: A4

Cholesterol reporter molecules.

2007

Cholesterol is a major constituent of the membranes in most eukaryotic cells where it fulfills multiple functions. Cholesterol regulates the physical state of the phospholipid bilayer, affects the activity of several membrane proteins, and is the precursor for steroid hormones and bile acids. Cholesterol plays a crucial role in the formation of membrane microdomains such as “lipid rafts” and caveolae. However, our current understanding on the membrane organization, intracellular distribution and trafficking of cholesterol is rather poor. This is mainly due to inherent difficulties to label and track this small lipid. In this review, we describe different approaches to detect cholesterol in …

Copia de vna carta, escrita al Padre Fray Alonso Sandin, de la Orden de Predicadores, Difinidor, y Procurador General de la Prouincia del Santo Rosar…

Inclou : Pvntos que hemos sabido se escriuen al Consejo contra nosotros este año, y respuesta à ellos... / del mismo Autor de la Carta, h. 8-14 Sign.: A-G2

Cholesterol Dependence of Collagen and Echovirus 1 Trafficking along the Novel α2β1 Integrin Internalization Pathway

2013

We have previously shown that soluble collagen and a human pathogen, echovirus 1 (EV1) cluster α2β1 integrin on the plasma membrane and cause their internalization into cytoplasmic endosomes. Here we show that cholesterol plays a major role not only in the uptake of α2β1 integrin and its ligands but also in the formation of α2 integrin-specific multivesicular bodies (α2-MVBs) and virus infection. EV1 infection and α2β1 integrin internalization were totally halted by low amounts of the cholesterol-aggregating drugs filipin or nystatin. Inhibition of cholesterol synthesis and accumulation of lanosterol after ketoconazole treatment inhibited uptake of collagen, virus and clustered integrin, an…

Viajando a la mercantil o apresado de ingleses piratas: dos formas de dar la vuelta al mundo a finales del siglo XVII y dos maneras de contarlo

2013

[EN] The paper analyses the accounts of two trips around the world conducted in the last two decades of the Seventeenth-century and published the one in Naples, 1699-1700, the other in mexico, a decade earlier. the first case was led by giovanni francesco gemelli Careri, a traveller vilified as fake by whom gave the final impetus to the genre of travel literature from french and British Enlightement. the second case was led by Alonso Ramirez, a traveller who was considered for three centuries just a fictional character. though two texts set in a peripheral place and time, from the point of view of European geopolitics of the moment and of the literary genre of travel writing as well, our ai…

L’elegia valenciana com a tòpic universal: la caiguda de València narrada des del Xarq al-Àndalus fins al romancer filipí

2016

Resum: Estudi de la formalització del concepte de València com a paradís perdut, des de la literatura àrab del Xarq al-Àndalus fins al romancer hispànic desenvolupat a l’arxipèlag filipí. S’hi analitza la conceptualització de l’elegia valenciana i el seu ús com a tòpic literari des de l’orient d’al-Àndalus fins a l’orient del món hispànic, explicant la formació del romancer filipí i la presència del Regne de València en les Lletres Filipines. S’hi estudien les elegies de la caiguda de la València islàmica a mans dels cristians, i com es formalitza una fascinació literària pel concepte de «València com a paradís», que s’exporta com a tòpic a una literatura asiàtica de base hispànica que té e…

Requirement of caveolae microdomains in extracellular signal-regulated kinase and focal adhesion kinase activation induced by endothelin-1 in primary…

1999

Endothelin-1 (ET-1) mitogenic activity in astrocytes is mediated by the activation of the extracellular signal-regulated kinase (ERK) pathway together with the Rho-dependent activation of the focal adhesion kinase (FAK) pathway. To clarify the mechanisms responsible for the coordinate activation of both pathways in the ET-1 signal propagation, the involvement of caveolae microdomains, suggested to play a role in signal transduction, was evaluated. In this study, it is reported that caveolae of primary astrocytes are enriched in endothelin receptor (ETB-R). Furthermore, signaling molecules such as the adaptor proteins Shc and Grb2, and the small G protein Rho, also reside within these microd…