Search results for "Flunitrazepam"

showing 3 items of 13 documents

1-Methyl-?-carboline (Harmane), a potent endogenous inhibitor of benzodiazepine receptor binding

1980

The interaction of several beta-carbolines with specific [3H]-flunitrazepam binding to benzodiazepine receptors in rat brain membranes was investigated. Out of the investigated compounds, harmane and norharmane were the most potent inhibitors of specific [3H]-flunitrazepam binding, with IC50-values in the micromolar range. All other derivatives, including harmine, harmaline, and several tetrahydroderivatives were at least ten times less potent. Harmane has been previously found in rat brain and human urine, so it is the most potent endogenous inhibitor of specific [3H]-flunitrazepam binding known so far, with a several fold higher affinity for the benzodiazepine receptor than inosine and hy…

Receptors DrugFlunitrazepamIn Vitro TechniquesPharmacologyRetinachemistry.chemical_compoundHarmalineAlkaloidsHarminemedicineAnimalsHarmaneInosineBenzodiazepine receptor bindingBrain ChemistryPharmacologybeta-CarbolineGABAA receptormusculoskeletal neural and ocular physiologyGeneral MedicineReceptors GABA-ARatsHarmineKineticschemistryBiochemistryCattleFlunitrazepammedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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Synthesis, benzodiazepine receptor binding and molecular modelling of isochromeno[4,3-c]pyrazol-5(1H)-one derivatives

2012

Abstract A series of isochromeno[4,3-c]pyrazole-5(1H)-one derivatives 7b–h were prepared and tested at 10 μM for their ability to displace specific [3H]flunitrazepam from bovine brain membranes. The substitution pattern of the above derivatives was shown to influence the receptor affinity. The most active compound of the series was 7e, showing a 54% inhibition of [3H]flunitrazepam binding. Compounds 7a–d,i were compared with the known isomers chromeno[4,3-c]pyrazole-4(1H)-ones 14a–d,i, showing that the isochromene/chromene isomerism influences the activity.

StereochemistryProtein ConformationChemistry Techniques SyntheticIsochromeno[43-c]pirazoles Dihydrospiro[isoindole-13’-pyrazol]-3(2H)- ones Benzodiazepine receptorDrug DiscoverymedicineAnimalsHumansBenzopyransReceptorBenzodiazepine receptor bindingPharmacologyChemistryOrganic ChemistryGeneral MedicineReceptors GABA-ASettore CHIM/08 - Chimica FarmaceuticaMolecular Docking SimulationMembraneBovine brainActive compoundPyrazolesCattleFlunitrazepam bindingFlunitrazepammedicine.drugProtein Binding
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?-Carboline binding indicates the presence of benzodiazepine receptor subclasses in the bovine central nervous system

1982

Receptor binding studies were performed with tritiated propyl β-carboline-3-carboxylate ([3H]PrCC), tritiated ethyl β-carboline-3-carboxylate ([3H]ECC), and tritiated flunitrazepam ([3H]FNT) in membrane preparations from different regions of the bovine brain and retina. Specific binding in all regions investigated was associated with benzodiazepine receptor sites. However, not all benzodiazepine receptor sites. However, not all benzodiazepine receptors in the regions investigated as determined by the specific binding of tritiated flunitrazepam ([3H]FNT) are available for [3H]PrCC suggesting that specific [3H]PrCC binding labels only one subclass or subpopulation of the benzodiazepine recept…

medicine.medical_specialtyAdenosineIndolesmedicine.drug_classReceptors DrugCentral nervous systemHippocampusSubstrate Specificitychemistry.chemical_compoundInternal medicinemedicineAnimalsReceptorgamma-Aminobutyric AcidBrain ChemistryPharmacologyBenzodiazepineBinding SitesGABAA receptorChemistrybeta-CarbolineGeneral MedicineReceptors GABA-Amedicine.anatomical_structureEndocrinologyGABAergicCattleFlunitrazepamCarbolinesmedicine.drugNaunyn-Schmiedeberg's Archives of Pharmacology
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