Search results for "Footprint"
showing 10 items of 121 documents
Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significa…
2000
The expression of the nuoA-N operon of Escherichia coli K-12, which encodes the proton-pumping NADH dehydrogenase I is modulated by growth phase-dependent regulation. Under respiratory growth conditions, expression was stimulated in early exponential, and to a lesser extent in late exponential and stationary growth phases. The stimulation in the early exponential growth phase was not observed in fis mutants, which are deficient for the growth phase-responsive regulator Fis. Neither the alternative sigma factor RpoS nor the integration host factor (IHF) are involved in growth phase-dependent regulation of this operon. When incubated with nuo promoter DNA, isolated Fis protein formed three re…
Requirement of the Lactobacillus casei MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway.
2009
ABSTRACTLactobacillus caseican metabolizel-malic acid via malolactic enzyme (malolactic fermentation [MLF]) or malic enzyme (ME). Whereas utilization ofl-malic acid via MLF does not support growth, the ME pathway enablesL. caseito grow onl-malic acid. In this work, we have identified in the genomes ofL. caseistrains BL23 and ATCC 334 a cluster consisting of two diverging operons,maePEandmaeKR, encoding a putative malate transporter (maeP), an ME (maeE), and a two-component (TC) system belonging to the citrate family (maeKandmaeR). Homologous clusters were identified inEnterococcus faecalis,Streptococcus agalactiae,Streptococcus pyogenes, andStreptococcus uberis. Our results show that ME is …
Synthetic environmental appraisal of waste management system: an application to the Sicilian region
2004
This paper addresses the problem of synthetically evaluating the environmental performances of urban solid waste systems. This, in fact, represents a crucial point in the management process of complex systems that local administrations are called to cope with, in order to provide decisions about policy options that involve different issues characterising the quality of life of people. Suitable methods of evaluation are clearly required for this aim. This work introduces the dashboard of sustainability and the ecological footprint approaches as aggregate indicators of the performances of solid waste management systems. The methods have been selected due to their intrinsic simplicities, provi…
Behind the Digital
2019
Fraschini gives a critical and interrogative view to digital design. He righly dwells much upon the tactile aspect of architecture. His reflections are grounded on Mario Carpo studies and very importantly he traces the idea of footprint as ground stone for architectural design.
Rapid evaluation of ammonium in different rain events minimizing needed volume by a cost-effective and sustainable PDMS supported solid sensor.
2020
Abstract The presence of ammonium ion in rainwater is due to atmospheric processes which involve its scavenging from gas phase and particulate matter. The fractionated samplings of rainwater can provide information about these processes and their potential sources. However, only a low sample volume may be available, which constrained the analysis in general and more particularly in situ mode. For minimizing this limitation, this work proposes a polydimethylsiloxane (PDMS)-salicylate sensor that produces a color change. The embedding of solid reagents into PDMS was optimized. Good analytical characteristics (analysis time of 10 min, sample volume of 500 μL, limit of detection 0.03 μgmL−1) we…
A Holistic Vision of Smart Cities: An Opportunity for a Big Change
2016
The depletion of energy resources on the one hand, and the population growth on the other, forced the society at all levels (local, national and international) to turn its attention to the identification of new forms of protection of the environment and the waste reduction for a new eco-sustainable way of living. The process of massive urbanization already in place, exacerbated by the movement of large masses of people in search of a more human form of life, is putting severely under test the livability within our cities, bringing out the inefficiency of existing management and organization models. Daisaku Ikeda said: “Certainly, the density of urban populations means that problems are conc…
Cities facing the Wild
2021
Untamed ecosystems and plots of wild nature increasingly constitute large parts of contemporary urban spaces. They are often the unbidden result of a long-standing lack of maintenance as well as of the uncontrolled flourishing of weeds produced by the pesticide absolute ban. But they are also something more. Many recently implemented urban open spaces deal with wild nature to solve some of the most urgent tasks of the contemporary cities: reclaiming areas fallen into disuse, designing sustainable infrastructures, revitalizing valuable public spaces, enhancing the ecological footprint of new developments, suggesting new practices and social ritual, reducing management costs, fighting climate…
Agder as Mutual Competence Builders: Developing Sustainability as a Competitive Advantage
2015
In this chapter, Agder as Mutual Competence Builders: developing sustainability as a competitive advantage, Karen Landmark, Marianne Rodvelt and Stina Torjesen provide a discussion of companies in the Agder region which are organised in the Eyde-network, and how they have developed a common sustainability agenda in co-operation with the university. The chapter shows how this is followed up by organising an Eyde-school as vocational training in co-operation with the university.
In silico characterization of the neural alpha tubulin gene promoter of the sea urchin embryo Paracentrotus lividus by phylogenetic footprinting
2011
During Paracentrotus lividus sea urchin embryo development one alpha and one beta tubulin genes are expressed specifically in the neural cells and they are early end output of the gene regulatory network that specifies the neural commitment. In this paper we have used a comparative genomics approach to identify con- served regulatory elements in the P. lividus neural alpha tubulin gene. To this purpose, we have first isolated a genomic clone containing the entire gene plus 4.5 Kb of 5 0 upstream sequences. Then, we have shown by gene transfer experiments that its non-coding region drives the spatio- temporal gene expression corresponding substantially to that of the endogenous gene. In addi…
Environmental management of granite slab production from an industrial ecology standpoint
2014
Abstract The granite production chain is high energy and water intensive with low resource efficiency. This paper focus on characterizing the potential for environmental improvement of the granite production chain by promoting the optimization of sawing technology, the implementation of rainwater harvesting (RWH) and the material recovery of wasted granite (sludge). The environmental performance of multi-blade gangsaw (MBGS) and diamond multi-wire saw (DMWS) technologies is compared by mean of life cycle assessment. Results demonstrate that DMWS technology contribute 30% of water savings, 40% of energy savings and 80% of material savings per square meter of polished granite tiles (60 x 40 x…