Search results for "Forming"

Vibrio cholerae cytolysin: assembly and membrane insertion of the oligomeric pore are tightly linked and are not detectably restricted by membrane fl…

2000

AbstractHemolytic strains of Vibrio cholerae secrete a cytolysin that, upon binding as a monomer, forms pentameric pores in animal cell membranes. Pore formation is inhibited at low temperature and in the absence of cholesterol. We here posed the following questions: firstly, can oligomerization be observed in the absence of pore formation? Secondly, is membrane fluidity responsible for the effect of temperature or of cholesterol upon pore formation? The first issue was approached by chemical cross-linking, by electrophoretic heteromer analysis, and by electron microscopy. None of these methods yielded any evidence of a non-lytic pre-pore oligomer. The second question was addressed by the u…

In-situ forming gel-like depot of a polyaspartamide-polylactide copolymer for once a week administration of Sulpiride

2015

Abstract Objectives An in-situ forming gel-like depot, prepared by using an appropriate polyaspartamide-polylactide graft copolymer, has been employed to release in a sustained way sulpiride. Methods α,β-poly(N-2-hydroxyethyl)-D,L-aspartamide-g-polylactic acid (PHEA-g-PLA) has been used as a polymer component. Its physicochemical properties make possible to dissolve it in N-methyl-2-pyrrolidone, with the obtainment of a solution able to form a gel-like depot once injected into a physiological medium. Cell compatibility of PHEA-g-PLA depot has been investigated, using murine dermal fibroblasts as cell model. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazo…

An Intelligent Tool to Predict Fracture in Sheet Metal Forming Operations

2007

One of the main issues in sheet metal forming operations design is the determination of formability limits in order to prevent necking and fracture. In fact, the ability to predict fracture represents a powerful tool to improve the production quality in mechanical industry. Many researchers investigated the problem here addressed, mainly studying forming limit diagrams (FLD) or developing fracture criteria which are able to foresee fracture defects for different processes. In this paper, the author present some early results of a research project focused on the application of artificial intelligence (AI) for ductile fracture prediction in sheet metal forming operations. The main advantage o…

Dielectric relaxation models applied of the dynamics of myoglobin as determined by Mössbauer spectroscopy

1996

Abstract Protein specific modes of motions are found in myoglobin crystals above 180 K. In this contribution we show that this type of motions can be analyzed by a Davidson-Cole, a Cole-Cole or a Havriliak-Negami distribution in analogy to dielectric relaxation. However, the temperature dependence of the obtained parameters is quite unusual indicating a broadening of the distributions with temperature instead of motional narrowing. This can be understood from the picture of conformational substates if one assumes that more and more substates become accessible with increasing temperature. The result shows that the analogy between glass forming organic liquids and proteins should not be exagg…

Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A

2014

Background: Important knowledge about the role of vitamin A in vertebrate heart development has been obtained using the vitamin A-deficient avian in ovo model which enables the in vivo examination of very early stages of vertebrate heart morphogenesis. These studies have revealed the critical role of the vitamin A-active form, retinoic acid (RA) in the regulation of several developmental genes, including the important growth regulatory factor, transforming growth factor-beta2 (TGFβ2), involved in early events of heart morphogenesis. However, this in ovo model is not readily available for elucidating details of molecular mechanisms determining RA activity, thus limiting further examination o…

Animales, teorías feministas posmodernas y prácticas artísticas.

2019

Este artículo pretende mostrar cómo algunas teorías feministas posmodernas como el ecofeminismo y el posthumanismo, de autoras como Françoise d'Eaubonne, Alicia Puleo, Donna Haraway o Rosi Baridotti han contribuido a la crítica contra las prácticas androcéntricas basadas en el dominio sistémico de devastación y explotación del otro/a/os, perpetuando la jerarquía y autoridad del hombre.  Frente a ello estas autoras han propuesto un nuevo modelo de relación orgánica y de convivencia entre cultura y naturaleza, capaz de superar los modelos binarios, inspirado en el respeto a la diversidad de sexos y especies.Estos principios se hayan, así mismo, en las prácticas artísticas de algunas autoras c…

L'Esperienza del Teatro Massimo di Palermo

2010

Le Opere e i Giorni

2010

Fondazioni Liriche e Finanziamenti Pubblici

2008

European Lifelong Guidance Policy Network representatives’ conceptions of the role of information and communication technologies related to national …

2015

This article reports findings from a phenomenographic investigation into European Lifelong Guidance Policy Network representatives’ conceptions of the role of information and communication technologies (ICT) related to national lifelong guidance policies. The role of ICT in relation to national lifelong guidance policies was conceived as (1) unexploited, (2) emerging, (3) acknowledged but fragmented, and (4) strategic. The hierarchical structure of the findings may serve as a tool to enable policy makers and other stakeholders to deepen their understanding of critical aspects that may have an important role in relation to further developments and the successful implementation of existing an…