Search results for "Forming"
showing 10 items of 1719 documents
Modulation of IL-1?, IL-6, IL-8, TNF-?, and TGF-? secretions by alveolar macrophages under NO2 exposure
2004
Activated alveolar macrophages (AMs) secrete interleukine (IL)1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β), whose inflammatory and fibroblast-activating characteristics may play a role in the maintenance of pulmonary inflammatory processes and subsequent fibrosis. Human AMs were transferred to a gas cylinder and exposed to NO2 in concentrations ranging from 0.1 to 0.5 ppm in synthetic air for 30 min at 37°C. AMs were fixed on a polycarbonate membrane and placed on culture medium. A culture was established, with the exposed AM (nonstimulated or stimulated with 1 μg/ml lipopolysaccharide [LPS]), and the remaining cells were used to determine the cy…
Impact of T-cell-mediated immune response on xenogeneic heart valve transplantation: short-term success and mid-term failure.
2017
Objectives Allogeneic frozen cryopreserved heart valves (allografts or homografts) are commonly used in clinical practice. A major obstacle for their application is the limited availability in particular for paediatrics. Allogeneic large animal studies revealed that alternative ice-free cryopreservation (IFC) results in better matrix preservation and reduced immunogenicity. The objective of this study was to evaluate xenogeneic (porcine) compared with allogeneic (ovine) IFC heart valves in a large animal study. Methods IFC xenografts and allografts were transplanted in 12 juvenile merino sheep for 1-12 weeks. Immunohistochemistry, ex vivo computed tomography scans and transforming growth fa…
Aclidinium inhibits human lung fibroblast to myofibroblast transition
2011
Background Fibroblast to myofibroblast transition is believed to contribute to airway remodelling in lung diseases such as asthma and chronic obstructive pulmonary disease. This study examines the role of aclidinium, a new long-acting muscarinic antagonist, on human fibroblast to myofibroblast transition. Methods Human bronchial fibroblasts were stimulated with carbachol (10 −8 to 10 −5 M) or transforming growth factor-β1 (TGF-β1; 2 ng/ml) in the presence or absence of aclidinium (10 −9 to 10 −7 M) or different drug modulators for 48 h. Characterisation of myofibroblasts was performed by analysis of collagen type I and α-smooth muscle actin (α-SMA) mRNA and protein expression as well as α…
Role of JAK/STAT in Interstitial Lung Diseases; Molecular and Cellular Mechanisms
2021
Interstitial lung diseases (ILDs) comprise different fibrotic lung disorders characterized by cellular proliferation, interstitial inflammation, and fibrosis. The JAK/STAT molecular pathway is activated under the interaction of a broad number of profibrotic/pro-inflammatory cytokines, such as IL-6, IL-11, and IL-13, among others, which are increased in different ILDs. Similarly, several growth factors over-expressed in ILDs, such as platelet-derived growth factor (PDGF), transforming growth factor β1 (TGF-β1), and fibroblast growth factor (FGF) activate JAK/STAT by canonical or non-canonical pathways, which indicates a predominant role of JAK/STAT in ILDs. Between the different JAK/STAT iso…
Optimizing auditory images and distance metrics for self‐organizing timbre maps*
1996
Abstract The effect of using different auditory images and distance metrics on the final configuration of a self‐organized timbre map is examined by comparing distance matrices, obtained from simulations, with a similarity rating matrix, obtained using the same set of stimuli as in the simulations. Gradient images, which are intended to represent idealizations of physiological gradient maps in the auditory pathway, are constructed. The optimal auditory image and distance metric, with respect to the similarity rating data, are searched using the gradient method.
Asymmetric Single Point Incremental Forming of Sheet Metal
2005
The use of computers in manufacturing has enabled the development of several new sheet metal forming processes, which are based upon older technologies. This paper describes modifications that have been made to traditional forming methods such as conventional spinning and shear forming, forming processes in which deformation is localized. Recent advances have enabled this localized deformation to be accurately controlled and studied. Current developments have been focused on forming asymmetric parts using CNC technology, without the need for costly dies. Asymmetric Incremental Sheet Forming has the potential to revolutionize sheet metal forming, making it accessible to all levels of manufac…
Incremental Forming Process for the Accomplishment of Automotive Details
2007
In the last decades the scenario of the industrial production is remarkably changed, since new market requirements have to be faced by the industries. The market, actually, more and more, asks for vary models and niches product. The necessity to intercept dynamically and to satisfy the demands for the market, driver of the innovation process, involves the necessity to reduce the Timeto- market introducing to new methodologies of engineering, like the 3D-prototyping, for the qualitative and structural analysis of the final component. For these reasons, at the beginning of the nineties, a new philosophy of sheet metal forming process begins to assert on the industrial scene, whose basic logic…
Pre- and Post-translational Regulation of Lysyl Oxidase by Transforming Growth Factor-β1 in Osteoblastic MC3T3-E1 Cells
1995
The final enzymatic step required for collagen cross-linking is the extracellular oxidative deamination of peptidyl-lysine and -hydroxylysine residues by lysyl oxidase. A cross-linked collagenous extracellular matrix is required for bone formation. The goals of this study were to compare the transforming growth factor (TGF)-beta 1 regulation of lysyl oxidase enzyme activity and steady state mRNA levels to changes in COL1A1 mRNA levels in MC3T3-E1 osteoblastic cells. TGF-beta 1 increased steady state lysyl oxidase and COL1A1 mRNA levels in a dose- and time-dependent manner. The increase in lysyl oxidase mRNA levels was transient, peaking at 12 h and 8.8 times controls in cells treated with 4…
Influence of Geometrical Parameters, Wall Angle and Part Shape on Thickness Reduction of Single Point Incremental Forming
2014
Abstract The aim of this paper is to study the thickness reduction during the whole single point incremental forming process. The experimental setup is realized in such a way that the blank sheet is in the vertical position and forming is done using an anthropomorphic robot with six degrees of freedom. The vertical positioning of the blank sheet allows the on-line visualization using an optical measuring system of the major and minor strains and of the thickness reduction. The wall angle varied between values of 45 0 , 55 0 and 65 0 respectively. The logarithmic major strain and minor strains and logarithmic thickness reduction were analyzed in order to allow a comparison with values obtain…
Meshless Simulation of Friction Stir Welding
2007
This paper encompasses our first efforts towards the numerical simulation of friction stir welding by employing a Lagrangian approach. To this end, we have employed a meshless method, namely the Natural Element Method (NEM). Friction Stir welding is a welding process where the union between the work pieces is achieved through the extremely high deformation imposed by a rotating pin, which moves between the two pieces. This extremely high strain is the main responsible of the difficulties associated with the numerical simulation of this forming process. Eulerian and Arbitrary Lagrangian-Eulerian (ALE) frameworks encounter difficulties in some aspects of the simulation. For instance, these ap…