Search results for "Forms"
showing 10 items of 668 documents
Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.
2004
Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, Gt, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca(2+)-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to Gtbetagamma heterodimers. High affinity b…
Differential occurrence of S100A7 in breast cancer tissues: A proteomic-based investigation
2012
Purpose The present study reports for the first time a large-scale proteomic screening of the occurrence, subcellular localization and relative quantification of the S100A7 protein among a group of 100 patients, clinically grouped for the diagnosis of infiltrating ductal carcinoma (IDC). Experimental design To this purpose, the methods of differential proteomics, Western blotting, and immunohistochemistry were used. Results The identity of two isoforms of the protein was assessed by mass spectrometry and immunologically confirmed. Moreover, we proved by immunocytochemical applications the exclusive localization of the protein within the neoplastic cells. The correlation of S100A7 expression…
Socially desirable responding:enhancement and denial in 20 countries
2015
This article investigated the dimensionality, measurement invariance, and cross-cultural variations of social desirability. A total of 3,471 university students from 20 countries completed an adapted version of the Marlowe–Crowne scale. A two-dimensional structure was revealed in the pooled sample, distinguishing enhancement (endorsement of positive self-description) and denial (rejection of negative self-description). The factor structure was supported in most countries; medium-sized item bias was found in two denial items. In a multilevel analysis, we found that (a) there was more cross-cultural variation in denial than enhancement; (b) females tended to score higher on enhancement where…
Localization of HSP70, Cdc2, and cyclin B in sea urchin oocytes in non-stressed conditions.
2003
In Paracentrotus lividus embryos, a Mediterranean sea urchin species, HSP70 is present in all the cells. During cell division it localizes under normal growth conditions on the centrosomes and on the whole isolated mitotic apparatus. Now, in situ hybridization, Western blot analyses, and immunohistochemistry show that the HSP70 mRNA is present in both small and large P. lividus oocytes, that all four isoforms of HSP70 can be found also in the oocytes, and that a certain amount of HSP70 localizes on asters and spindles during polar body formation. Moreover, two representative cell-cycle related proteins, cyclin B, and Cdc2, are present both in small and large oocytes, concentrating in the ge…
SEDIMENTARY DYNAMICS OF THE MARETTIMO CONTINENTAL SHELF AND MARETTIMO CHANNEL (EGADI ISLANDS – WESTERN MEDITERRANEAN): ANALYSIS OF DEPOSITIONAL AND E…
2011
Sedimentary dynamics of the Marettimo continental shelf and Marettimo Channel (Egadi Island – Western Mediterranean), have been unveiled based on the erosive and depositional features observed at the seafloor. Different morphologic features were recognized through side scan sonar, multibeam and high-resolution seismics, acquired in the framework of national Research Projects (CARG, GebecSud, MaGIC) carried out by the Department of Earth and Marine Science (former Department of Geology and Geodesy) of the University of Palermo. Sediment samples have been also collected and analyzed. The Egadi Islands are part of the NW Sicilian offshore, and belong to the mountain chain that connects the Sic…
Time-Frequency Filtering for Seismic Waves Clustering
2014
This paper introduces a new technique for clustering seismic events based on processing, in time-frequency domain, the waveforms recorded by seismographs. The detection of clusters of waveforms is performed by a k-means like algorithm which analyzes, at each iteration, the time-frequency content of the signals in order to optimally remove the non discriminant components which should compromise the grouping of waveforms. This step is followed by the allocation and by the computation of the cluster centroids on the basis of the filtered signals. The effectiveness of the method is shown on a real dataset of seismic waveforms.
Sesquilinear forms associated to sequences on Hilbert spaces
2019
The possibility of defining sesquilinear forms starting from one or two sequences of elements of a Hilbert space is investigated. One can associate operators to these forms and in particular look for conditions to apply representation theorems of sesquilinear forms, such as Kato's theorems. The associated operators correspond to classical frame operators or weakly-defined multipliers in the bounded context. In general some properties of them, such as the invertibility and the resolvent set, are related to properties of the sesquilinear forms. As an upshot of this approach new features of sequences (or pairs of sequences) which are semi-frames (or reproducing pairs) are obtained.
One precursor, three apolipoproteins: The relationship between two crustacean lipoproteins, the large discoidal lipoprotein and the high density lipo…
2014
The novel discoidal lipoprotein (dLp) recently detected in the crayfish, differs from other crustacean lipoproteins in its large size, apoprotein composition and high lipid binding capacity, We identified the dLp sequence by transcriptome analyses of the hepatopancreas and mass spectrometry. Further de novo assembly of the NGS data followed by BLAST searches using the sequence of the high density lipoprotein/1-glucan binding protein (HDL-BGBP) of Astacus leptodactylus as query revealed a putative precursor molecule with an open reading frame of 14.7 kb and a deduced primary structure of 4889 amino acids. The presence of an N-terminal lipid bind- ing domain and a DUF 1943 domain suggests the…
Shedding of Membrane Vesicles Mediates Fibroblast Growth Factor-2 Release from Cells
2003
Fibroblast growth factor-2 (FGF-2), a polypeptide with regulatory activity on cell growth and differentiation, lacks a conventional secretory signal sequence, and its mechanism of release from cells remains unclear. We characterized the role of extracellular vesicle shedding in FGF-2 release. Viable cells released membrane vesicles in the presence of serum. However, in serum-free medium vesicle shedding was dramatically down-regulated, and the cells did not release FGF-2 activity into their conditioned medium. Addition of serum to serum-starved cells rapidly induced intracellular FGF-2 clustering under the plasma membrane and into granules that colocalized with patches of the cell membrane …
A framework for IoT service experiment platforms in smart-city environments
2016
Cities provide an excellent platform for gathering and detection of massive amount of data from cities and citizens. Emergence of new digital technologies inspires not only city governments but also city residents, researchers, companies and other stakeholders in discovering and creating new innovative solutions to solve urban challenges and improve peoples´ everyday life. Developing novel Internet of Things (IoT) solutions for cities and citizens requires facilities where IoT applications and services can be tested and experimented. The challenge for many smart-city test and experimentation platforms (TEPs), like living labs, has been the lack of sustainable value creation model. This has …