Search results for "Fragment"
showing 10 items of 1612 documents
Clonal heterogeneity of the growth and invasive response of a human breast carcinoma cell line to parathyroid hormone-related peptide fragments
1997
It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone (PTH)-related peptide (PTHrP) and PTH/PTHrP receptor (PTH/PTHrP-R) genes, that N-terminal, mid-regional and C-terminal immunoreactive PTHrP can be found in cell conditioned medium and, furthermore, that exogenously added PTHrP (1-34), (67-86) and, to a minor extent, (107-139) are anti-mitogenic but promote Matrigel invasion by this cell line. It has also been reported that PTHrP gene expression is selectively switched on in those 8701-BC clonal lines endowed with a higher proliferation rate and invasive ability in vitro. Here we have first examined t…
Modulation of type II collagen-induced arthritis in DBA/1 mice by intravenous application of a peptide from the C1q-A chain.
1992
In this report we are able to show that intravenous (i.v.) application (day 0) of a nonapeptide (residues 26-34) from the human C1q A-chain (designated peptide A-C1q) prior to intradermal (i.d.) administration of chicken type II collagen (CII) in arthritis-susceptible DBA/1 mice (H2q), leads to abrogation of polymorphonuclear neutrophil (PMN) invasion into the joints. This nonapeptide exhibits epitope characteristics and high homology to residues 137-147 of CB11 (a cyanogen bromide fragment of chicken CII, known to contain both arthritis inducing and suppressing determinants). Arthritis index was lowest in animals pretreated i.v. with CII (as internal control), though animals pretreated i.v…
Blocks to, and building blocks of, narrativity : fragments, anecdotes, and narrative lines in David Markson’s Reader’s block
2017
David Markson’s Reader’s block (1996) consists of 193 pages of quotations, anecdotes, names, and fragments. The book bears the paratext “A novel,” and the work has indeed been read as a narrative whole, in which “an aging author [...] contemplates the writing of a novel.” By being out of ordinary and therefore worth of telling, the anecdotes or curiosities seemingly fulfill the requirements of a “natural” narrative as defined by Monika Fludernik (1996). However, a mass of such mini-narratives, mixed with even more fragmentary texts, seems to defy narrativity (and tellability). In my reading, the ostensive block to narrativity also functions as its very building block. Thanks to polysemy, bl…
Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine
2010
Jonna Nykky, Jenni E Tuusa, Sanna Kirjavainen, Matti Vuento, Leona GilbertNanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, FinlandAbstract: Viruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was f…
Mass measurements of As, Se, and Br nuclei, and their implication on the proton-neutron interaction strength toward the N=Z line
2021
Mass measurements of the $^{69}$As, $^{70,71}$Se and $^{71}$Br isotopes, produced via fragmentation of a $^{124}$Xe primary beam at the FRS at GSI, have been performed with the multiple-reflection time-of-flight mass spectrometer (MR-TOF-MS) of the FRS Ion Catcher with an unprecedented mass resolving power of almost 1,000,000. For the $^{69}$As isotope, this is the first direct mass measurement. A mass uncertainty of 22 keV was achieved with only 10 events. For the $^{70}$Se isotope, a mass uncertainty of 2.6 keV was obtained, corresponding to a relative accuracy of $\delta$m/m = 4.0$\times 10^{-8}$, with less than 500 events. The masses of the $^{71}$Se and $^{71}$Br isotopes were measured…
Semantic Complexity in Natural Language
2015
This chapter presents the technical framework that the authors used to define fragments of natural languages and formulate questions as to their semantic complexity. It examines the study of the classical syllogistic and its extensions. It analyzes the semantic complexity of various salient fragments of English. It highlights that the language of argument, featuring transitive verbs, is in an objective sense inferentially no more complex than the language of classical syllogisms exemplified by argument, indeed, the analogous extension featuring ditransitive verbs involves only a modest increase in complexity. On the other hand, the language of argument, which adds relative clauses to the cl…
The role of SAXS and molecular simulations in 3D structure elucidation of a DNA aptamer against lung cancer
2021
Aptamers are short, single-stranded DNA or RNA oligonucleotide molecules that function as synthetic analogs of antibodies and bind to a target molecule with high specificity. Aptamer affinity entirely depends on its tertiary structure and charge distribution. Therefore, length and structure optimization are essential for increasing aptamer specificity and affinity. Here, we present a general optimization procedure for finding the most populated atomistic structures of DNA aptamers. Based on the existed aptamer LC-18 for lung adenocarcinoma, a new truncated LC-18 (LC-18t) aptamer LC-18t was developed. A three-dimensional (3D) shape of LC-18t was reported based on small-angle X-ray scattering…
Structure‐ and Interaction‐Based Design of Anti‐SARS‐CoV‐2 Aptamers
2022
Aptamer selection against novel infections is a complicated and time-consuming approach. Synergy can be achieved by using computational methods together with experimental procedures. This study aims to develop a reliable methodology for a rational aptamer in silico et vitro design. The new approach combines multiple steps: (1) Molecular design, based on screening in a DNA aptamer library and directed mutagenesis to fit the protein tertiary structure; (2) 3D molecular modeling of the target; (3) Molecular docking of an aptamer with the protein; (4) Molecular dynamics (MD) simulations of the complexes; (5) Quantum-mechanical (QM) evaluation of the interactions between aptamer and target with …
La fragmentation de l'orthographe française : le cas de Twitter
2018
The enormous development of new communication technologies, especially the Internet, causes an unprecedented reversal in our lives sińce the discovery of printer. Apart from their impact on interpersonal relationships, new communication technologies have also modified our writing practices. It is evident that the French language used in computer-mediated communication digresses more and more from the developed formal speeches, in particular by its “fragmented” orthographic form which approaches its o rai form. Although this “fragmented” writing is scorned by many educated people, these orthographic variants represent, according to us, the true state of the French language as used by native …
$\Lambda_c^{\pm}$ production in pp collisions with a new fragmentation function
2020
Physical review / D D 101(11), 114021 (2020). doi:10.1103/PhysRevD.101.114021