Search results for "Frame"
showing 10 items of 1767 documents
Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy
2020
AbstractThe current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and t…
Characterization of a new member of the sea urchin Paracentrotus lividus hsp70 gene family and its expression
1992
We have sequenced a second gene of the hsp70 family derived from a genomic clone of the sea urchin, Paracentrotus lividus. The structure of this gene, named hsp70IV gene, is interrupted by one intron and differs from the previously analyzed sea urchin hsp70II gene, which contains several introns. Two open reading frames of hsp70IV gene encode a predicted protein of 639 amino acids with an M(r) of 69,672. The 5' flanking region of the gene contains a putative TATA element, three heat-shock elements made up of some arrays of the 5-bp units, NGAAN and NTTCN (N = A,C,G or T), a canonic consensus sequence for binding of the regulatory activating transcription factor (ATF), and a purine box. The …
Nucleotide sequence of a ssRNA phage from Acinetobacter: kinship to coliphages.
2002
The complete nucleotide sequence of ssRNA phage AP205 propagating in Acinetobacter species is reported. The RNA has three large ORFs, which code for the following homologues of the RNA coliphage proteins: the maturation, coat and replicase proteins. Their gene order is the same as that in coliphages. RNA coliphages or Leviviridae fall into two genera: the alloleviviruses, like Qβ, which have a coat read-through protein, and the leviviruses, like MS2, which do not have this coat protein extension. AP205 has no read-through protein and may therefore be classified as a levivirus. A major digression from the known leviviruses is the apparent absence of a lysis gene in AP205 at the usual positio…
A sequence element downstream of the yeast HTB1 gene contributes to mRNA 3' processing and cell cycle regulation.
2002
Histone mRNAs accumulate in the S phase and are rapidly degraded as cells progress into the G(2) phase of the cell cycle. In Saccharomyces cerevisiae, fusion of the 3' untranslated region and downstream sequences of the yeast histone gene HTB1 to a neomycin phosphotransferase open reading frame is sufficient to confer cell cycle regulation on the resulting chimera gene (neo-HTB1). We have identified a sequence element, designated the distal downstream element (DDE), that influences both the 3'-end cleavage site selection and the cell cycle regulation of the neo-HTB1 mRNA. Mutations in the DDE, which is located approximately 110 nucleotides downstream of the HTB1 gene, lead to a delay in the…
Geodetic data analysis of VGOS experiments
2021
Very Long Baseline Interferometry (VLBI) serves as one of the common geodetic methods to define the global reference frames and monitor Earth's orientation variations. The technical upgrade of the VLBI method known as the VLBI Global Observing System (VGOS) includes a critical re-design of the observed frequencies from the dual band mode (S and X band, i.e. 2 GHz and 8 GHz) to observations in a broadband (2 – 14 GHz). Since 2019 the first VGOS experiments are available for the geodetic analysis in free access at the International VLBI service for Geodesy and Astrometry (IVS). Also regional-only subnetworks such as European VLBI stations have succeeded already in VGOS mode. Based on these br…
Interbasin Water Transfers in Spain: Interregional Conflicts and Governance Responses
2014
Semiarid and drought-prone Spain has managed to meet ever-increasing water demands for more than 50 years through the construction of publicly funded hydraulic infrastructure. Interbasin water transfers are the most expensive and complex supply-side tool used. They are also the most controversial, often associated with such unintended consequences as deteriorating freshwater ecosystems, disappearing recreational opportunities provided by aquatic ecosystems, and the loss of development opportunities for downstream communities. This situation has become increasingly unstable over the past decade due to the scarcity of new supply augmentation alternatives, political changes involving European …
Giustizia ambientale e recupero delle aree verdi: il caso di Palermo
2021
L’urban shrinkage è diventata una questione centrale tanto nel dibattito scientifico quanto nell’agenda politica di diverse città europee. Secondo i dati EUROSTAT (2019), circa il 40% delle città europee con più di 200.000 abitanti stanno perdendo popolazione; una contrazione che si esplicita maggiormente, sotto il profilo spaziale, in una progressiva sottoutilizzazione del patrimonio edificato, così come nel contestuale incremento di aree e terreni in stato di abbandono. In questo quadro controverso di mutazione strutturale della città, così come l’abbiamo conosciuta, le politiche urbane alla prova in Europa tentano di declinare la decrescita quale occasione strategica di investimento sull…
OLAP Personalization with User-Describing Profiles
2010
In this paper we have highlighted five existing approaches for introducing personalization in OLAP: preference constructors, dynamic personalization, visual OLAP, recommendations with user session analysis and recommendations with user profile analysis and have analyzed research papers within these directions. We have pointed out applicability of personalization to OLAP schema elements in these approaches. The comparative analysis has been made in order to highlight a certain personalization approach. A new method has been proposed, which provides exhaustive description of interaction between user and data warehouse, using the concept of Zachman Framework [1, 2], according to which a set of…
How to Survive the Anthropocene: Adaptive Atheism and the Evolution of Homo deiparensis
2015
Published version of an article from the journal: Religions. Also available from the publisher: http://dx.doi.org/10.3390/rel6020724 Why is it so easy to ignore the ecological and economic crises of the Anthropocene? This article unveils some of the religious biases whose covert operation facilitates the repression or rejection of warnings about the consequences of extreme climate change and excessive capitalist consumption. The evolved defaults that are most relevant for our purposes here have to do with mental credulity toward religious content (beliefs about supernatural agents) and with social congruity in religious contexts (behaviors shaped by supernatural rituals). Learning how to co…
The wheel of collaboration tools
2006
Paper from the 2006 20th anniversary conference on Computer supported cooperative work We present a holistic framework for analyzing and specifying collaboration solutions, developed by an oil and gas company in response to practical needs in supporting integrated collaboration and information management. A typology of collaboration tool capabilities, termed the Wheel of Collaboration Tools (WCT), is described. We assess its contributions, and discuss areas of application and potential further development. Our intent is to stimulate discussion and research related to this type of collaboration modeling.