Search results for "Frames"
showing 10 items of 265 documents
A frame shift mutation in a hot spot region of the nuclear autoantigen La (SS-B).
1996
A hot spot region was identified in the exon 7 of the nuclear autoantigen La (SS-B). Two La cDNAs were identified which contained a frame shift mutation in the hot spot region. One La cDNA was isolated from a cDNA library made from peripheral blood lymphocytes of an autoimmune patient with primary Sjogren's Syndrome, the other La cDNA was isolated from a human liver cDNA library. The patient's La cDNA had a deletion and the liver La cDNA had an insert of an (A)-residue at the same position. Inserts of 4, 16 and 24 more or less homogeneous (A)-residues were found at the same site in the three La retropseudogenes. The hot spot region located in one of the major autoepitope regions of the La a…
Analysis of expression of an alternative La (SS-B) cDNA and localization of the encoded N- and C-terminal peptides
1997
AbstractA deletion of an (A)-residue was detected in a cDNA encoding for the nuclear autoantigen La/SS-B. The cDNA was recently isolated from a cDNA library made from peripheral blood lymphocytes of a patient with primary Sjögren's Syndrome. The region, where the deletion occurred, represents a hot spot region in the La gene(s). It leads to a frame shift mutation and a premature stop codon eleven amino acids downstream of the deletion site within one of the protease sensitive regions of the La protein. In spite of the frame shift mutation expression of full length La protein occurred efficiently in E. coli. Full length La protein was also made in SF9 cells infected with recombinant baculovi…
An insect juvenile hormone-specific epoxide hydrolase is related to vertebrate microsomal epoxide hydrolases.
1996
Abstract We describe the first cDNA sequence encoding a juvenile hormone-specific epoxide hydrolase from an insect. A full-length cDNA clone revealed a 462-amino-acid open reading frame encoding an amino acid sequence with 44% identity and 64% similarity to human microsomal epoxide hydrolase. All residues in the catalytic triad (residues Asp 227 -His 428 -Asp 350 in the M. sexta protein) were present, as was the conserved Trp 154 corresponding to the oxyanion hole. The surprising similarity of insect juvenile hormone epoxide hydrolase to vertebrate microsomal epoxide hydrolases, coupled with the ancient lineage of the epoxide hydrolases and haloalkane dehalogenases, suggests that this catab…
Cloning and expression of a cDNA copy of the viral K28 killer toxin gene in yeast
1995
The killer toxin K28, secreted by certain killer strains of the yeast Saccharomyces cerevisiae is genetically encoded by a 1.9 kb double-stranded RNA, M-dsRNA (M28), that is present within the cell as a cytoplasmically inherited virus-like particle (VLP). For stable maintenance and replication, M28-VLPs depend on a second dsRNA virus (LA), which has been shown to encode the major capsid protein (cap) and a capsid-polymerase fusion protein (cap-pol) that provides the toxin-coding M-satellites with their transcription and replicase functions. K28 toxin-coding M28-VLPs were isolated, purified and used in vitro for the synthesis of the single-stranded M28 transcript, which was shown to be of pl…
Molecular cloning of rat G-protein-coupled receptor kinase 6 (GRK6) from brain tissue, and its mRNA expression in different brain regions and periphe…
1997
The rat G-protein-coupled receptor kinase 6 (GRK6) cDNA was cloned from rat brain tissue by a combination of reverse-transcription polymerase chain reactions (RT-PCR), based on homology to the cloned human GRK6, and rapid amplification of cDNA ends (RACE-PCR). We obtained a clone of 2817 bp with an open reading frame of 1731 bp encoding for a protein of 576 amino acids that is 96.7% identical and 97.9% similar to its human counterpart. mRNA was detectable in all brain areas examined. In addition, GRK6 was expressed in skeletal muscle, small intestine, aorta, liver, heart, lung, thymus, stomach, uterus and kidney.
Targeted steel frames by means of innovative moment resisting connections
2021
Abstract The present paper proposes the use of stepped cross section devices on steel frames aiming at reproducing a pre-established target push-over curve. To this aim a Limited Resistance Plastic Device (LRPD) to be inserted along selected structural members is proposed. The following two main specific features for LRPD are required: any elastic flexural stiffness variation of the original selected member must be avoided; an ultimate plastic bending moment value equal to an assigned percentage of the original limit resistance value must be ensured. Steel frames equipped with LRPD are modeled by means of an extension of a recently proposed Fibre Smart Displacement Based (FSDB) beam element…
Evaluation of infilled frames: an updated in-plane-stiffness macro-model considering the effects of vertical loads
2015
The influence of masonry infills on the in-plane behaviour of RC framed structures is a central topic in the seismic evaluation and retrofitting of existing buildings. Many models in the literature use an equivalent strut member in order to represent the infill but, among the parameters influencing the equivalent strut behaviour, the effect of vertical loads acting on the frames is recognized but not quantified. Nevertheless a vertical load causes a non-negligible variation in the in-plane behaviour of infilled frames by influencing the effective volume of the infill. This results in a change in the stiffness and strength of the system. This paper presents an equivalent diagonal pin-jointed…
Prediction of the additional shear action on frame members due to infills
2014
Infill masonry walls in framed structures make a significant contribution to the response under seismic actions. With special regard to reinforced concrete (RC) structures, it is known that internal forces modifications caused by the frame–infill interaction may be not supported by the surrounding frame because of the additional shear forces arising at the ends of beams and columns. Such additional forces may lead to the activation of brittle collapse mechanisms and hence their prediction is basic in capacity assessment, especially for structures that disregard the details for seismic zones. In this paper a parametric study is carried out addressed to the prediction of the shear forces ment…
Seismic Performance of Masonry Infilled RC Structures via N2 Pushover Assessment Procedures: Outcomes under Different Modeling Hypotheses of a Case S…
2016
The assessment of the capacity of RC masonry infilled RC structures constitutes nowadays a still debated issue. Pushover based procedures for the evaluation of seismic performance, such as N2 method, are largely used in practice and in force in several technical codes. The latter has proved to be reliable for a large number of structural typologies, however in the case of infilled frames, the choices made on the modelling strategy may radically modify the outcomes observable from the capacity curves and the consequent performance levels achievable. In the paper, the extent of different modelling choices on the results of the application of N2 procedure is investigated by the deep analysis o…
The use of wood with an anti-seismic function in the architecture of palermo during the 18th century
2016
The study analyzes the interventions in the historical buildings after the earthquakes that struck Palermo in 1726 and 1751, with a particular attention to the anti-seismic reinforcements. The research, based primarily on archival documents, focuses on a series of interventions in the architectural heritage, in which the use of wood for structural elements, was able to guarantee for an “antiseismic” reconstruction. Although wood was not commonly used as other materials (iron, for instance) in the post-earthquake reconstruction process of Palermo it was certified its use as an anti-seismic system in the reinforcements of the damaged buildings. It is documented, for instance, the insertion of…