Search results for "Freeze Drying"

showing 4 items of 34 documents

A way to follow the viability of encapsulated Bifidobacterium bifidum subjected to a freeze-drying process in order to target the colon: Interest of …

2012

The aim of this work was to apply flow cytometry in order to assess and compare the viability of freeze-dried entrapped bacteria with an usual technique by quantification by plate count techniques. It also aimed at studying the effect of various cryoprotectants on the viability of an entrapped Bifidobacterium bifidum subjected to freeze-drying to check their ability to be delivered all along the gastro-intestinal tract. The alginate-pectinate beads were chosen as the encapsulation matrix added with different protectants. The beads were characterized by scanning electron microscopy and the viability was checked by both methods. The best combination to improve viability of entrapped bacteria …

Sodium ascorbateCryoprotectantAlginatesColonved/biology.organism_classification_rank.speciesPharmaceutical ScienceFlow cytometryFreeze-dryingchemistry.chemical_compoundCryoprotective AgentsGlucuronic AcidmedicineGlycerolViability assayBifidobacteriumMicrobial ViabilityBifidobacterium bifidumChromatographybiologymedicine.diagnostic_testved/biologyHexuronic AcidsFlow Cytometrybiology.organism_classificationMolecular biologyBacterial LoadFreeze DryingchemistryPectinsBifidobacteriumEuropean Journal of Pharmaceutical Sciences
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Osmotic stress affects the stability of freeze-dried Lactobacillus buchneri R1102 as a result of intracellular betaine accumulation and membrane char…

2014

Aims To help cells to better resist the stressful conditions associated with the freeze-drying process during starter production, we investigated the effect of various osmotic conditions on growth, survival and acidification activity of Lactobacillus buchneri R1102, after freeze-drying and during storage for 3 months at 25°C. Methods and Results High survival rates during freeze-drying, but not during storage, were obtained when 0·1 mol l−1 KCl was added at the beginning of fermentation, without any change in membrane properties and betaine accumulation. This condition made it possible to maintain a high acidification rate throughout the process. In contrast, the addition of 0·6 mol l−1 KCl…

[SDV.BIO]Life Sciences [q-bio]/BiotechnologyOsmotic shockMembrane FluidityPreservation BiologicalBiologyApplied Microbiology and BiotechnologysurvivalPotassium Chloride03 medical and health scienceschemistry.chemical_compoundBetaineOsmotic PressureLactobacillusMembrane fluidityOsmotic pressure[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyLactobacillus buchneriFood sciencemembrane[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology030304 developmental biologyLactobacillus buchneri0303 health sciencesMicrobial Viability030306 microbiology[ SDV.BIO ] Life Sciences [q-bio]/BiotechnologyGeneral MedicineHydrogen-Ion Concentrationbiology.organism_classificationBetaineLactobacillusFreeze DryingchemistryBiochemistry13. Climate actionFermentationacidification activityFermentationosmotic stressIntracellularBiotechnology
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Microstructure and characteristic properties of dogfish skin gelatin gels prepared by freeze/spray-drying methods

2020

International audience; The effects of two pretreatments (microwaves or oven-drying) on the dogfish (Squalus acanthias) skin as well as two drying processes (freeze-drying or spray-drying) on the extracted gelatins were studied. Thus six types of gelatins were obtained, three of which were freeze-dried (FG) and the others were spray-dried (SG), from the untreated skin (US), microwaves-pretreated skin (MS) and oven-pretreated skin (OS). The highest yield (8.67%) was obtained for the OSFG, while the lowest one (3.06%) was measured for the OSSG. Interestingly, all gelatins exhibited relatively high protein (84.02-89.53%), and low lipid (0.50-1.71%) and ash (3.05-7.17%) contents. In addition, g…

food.ingredientDogfish skinColor02 engineering and technologyBiochemistryGelatinPhysico-chemical properties03 medical and health scienceschemistry.chemical_compoundIngredientfoodSqualus acanthiasHardnessStructural BiologyAmideSpectroscopy Fourier Transform InfraredAnimalsTransition TemperatureAmino AcidsDesiccationMicrowavesMicrostructureMolecular BiologySkin030304 developmental biology0303 health sciencesChromatographyCalorimetry Differential ScanningHigh proteinGeneral Medicine021001 nanoscience & nanotechnologyMicrostructureAmidesFreeze DryingchemistryDogfishYield (chemistry)Spray dryingMicroscopy Electron ScanningGelatin0210 nano-technologyGels[SDV.AEN]Life Sciences [q-bio]/Food and NutritionInternational Journal of Biological Macromolecules
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Optimization of Spray-Drying Process of

2019

Jerusalem artichoke is an important natural matrix for inulin production. In this experiment, response surface methodology (RSM) was employed to optimize the spray-drying parameters in order to determine the maximal inulin yield. For this study, three independent variables (heating temperature (Tª, 110–120 °C), creep speed (V, 18–22 rpm) and pressure (P, 0.02–0.04 MPa)) were used in the experimental design. Using the Box–Behnken design, the optimal parameters obtained were: drying temperature 114.6 °C, creep speed 20.02 rpm, and pressure: 0.03 MPa. The inulin yield, water content and particle size of inulin obtained by spray-drying and freeze-drying were compared. In this regard, the spray-…

response surface methodologyFreeze DryinginulinPlant ExtractsChemistry PharmaceuticalJerusalem artichokeHelianthusspray-dryingPowdersArticleMolecules (Basel, Switzerland)
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