Search results for "Fresh Water"
showing 10 items of 190 documents
Fresh water, marine and terrestrial cyanobacteria display distinct allergen characteristics.
2017
During the last decades, global cyanobacteria biomass increased due to climate change as well as industrial usage for production of biofuels and food supplements. Thus, there is a need for thorough characterization of their potential health risks, including allergenicity. We therefore aimed to identify and characterize similarities in allergenic potential of cyanobacteria originating from the major ecological environments. Different cyanobacterial taxa were tested for immunoreactivity with IgE from allergic donors and non-allergic controls using immunoblot and ELISA. Moreover, mediator release from human FceR1-transfected rat basophilic leukemia (RBL) cells was measured, allowing in situ ex…
Analytical insight into degradation processes of aminopolyphosphonates as potential factors that induce cyanobacterial blooms
2017
Aminopolyphosphonates (AAPs) are commonly used industrial complexones of metal ions, which upon the action of biotic and abiotic factors undergo a breakdown and release their substructures. Despite the low toxicity of AAPs towards vertebrates, products of their transformations, especially those that contain phosphorus and nitrogen, can affect algal communities. To verify whether such chemical entities are present in water ecosystems, much effort has been made in developing fast, inexpensive, and reliable methods for analyzing phosphonates. However, unfortunately, the methods described thus far require time-consuming sample pretreatment and offer relatively high values of the limit of detect…
An outbreak ofShewanella putrefaciensgroup in wild eelsAnguilla anguillaL. favoured by hypoxic aquatic environments
2016
Microbiological analyses were conducted on wild eels from the L′Albufera Lake (Spain). A total of 174 individuals were collected in two surveys (i.e. year 2008 and autumn–winter 2014) among those caught by local fishermen into the lagoon. The prevalence of Shewanella putrefaciens group was 1.7% in 2008 and rose above 32% in 2014. It was due to an outbreak of shewanellosis that presented a morbidity rate of 64%. S. putrefaciens group strains were isolated as pure cultures from the sick eels that showed white ulcers surrounded by a reddish inflammation, damage of the mouth, extensive skin discoloration, exophthalmia, ascites and bad odour. The S. putrefaciens group was recovered from freshwat…
Achlya spiralis, a new aquatic oomycete with bent oogonial stalks, isolated from the Burgundian region of France
2008
Achlya spiralis sp. nov. was isolated from water samples collected in the river Tille in the Burgundian region of France. The new oomycete is described, illustrated and compared with related species of the genus Achlya. It is characterized by the presence of smooth-walled oogonia that are usually borne on bent or twisted oogonial stalks; mainly monoclinous, androgynous and diclinous antheridial branches and eccentric oospores which generally do not mature or mature after a long period of time. The internal transcribed spacer (ITS) region of its rRNA is comprised of 671 bases. The taxonomic description of this new species, its comparison with related oomycetes and the sequence of the ITS reg…
''Sex Difference In Open-water Ultra-swim Performance In the Longest Freshwater Lake Swim In Europe''
2013
Eichenberger, Evelyn | Knechtle, Beat | Knechtle, Patrizia | Ruest, Christoph A. | Rosemann, Thomas | Lepers, Romuald | Senn, Oliver; International audience; ''This study examined participation and performance trends in the 26.4-km open-water ultra-swim "Marathon Swim in Lake Zurich," Switzerland. A total of 461 athletes (157 women and 304 men) finished the race between 1987 and 2011. The mean age of the finishers during the studied period was 32.0 +/- 6.5 years for men and 30.9 +/- 7.2 years for women. The mean age of finishers and the age of winners increased significantly across years for both sexes (p 0.05). The swimming time performance remained stable (p > 0.05) for both sexes across …
Carotenoids of two freshwater amphipod species (Gammarus pulex and G. roeseli) and their common acanthocephalan parasite Polymorphus minutus
2004
Carotenoid compositions of two freshwater Gammarus species (Crustacea: Amphipoda) and of their common acanthocephalan parasite Polymorphus minutus were characterized. The effect of carotenoid uptake by the parasite was addressed by comparing the carotenoid content of uninfected and infected female hosts. Using high-pressure liquid chromatography (HPLC), co-chromatography of reference pigments and electron ionization mass spectrometry of collected HPLC fractions (EI-MS), several xanthophylls and non-polar compounds were identified. Seven kinds of carotenoids, mainly xanthophylls, were identified in gammarids. Astaxanthin was predominant, amounting to 40 wt.% of total carotenoid in both uninf…
Cercarial Production of the Trematode Rhipidocotyle fennica in Clams Kept in the Field
1998
The numbers of cercariae that trematodes produce have been previously investigated in the laboratory but not in the field. I studied cercarial production of the bucephalid trematode Rhipidocotyle fennica in the freshwater unionid clam Anodonta piscinalis kept under natural conditions. Naturally infected clams (n = 180) were collected and marked in early June 1996. Every 14 days, starting from the collection date and ending in October, these clams were taken to the laboratory where they were monitored for the emergence of trematode cercariae. Between monitoring dates, the clams were returned to the collection site. From a random subsample of infected clams (n = 12), the number of cercariae p…
Determination of the herbicide benfuresate by its photo-induced chemiluminescence using flow multicommutation methodology.
2007
The present paper deals with an analytical strategy based on coupling photo-induced chemiluminescence in a multicommutation continuous-flow methodology for the determination of the herbicide benfuresate. The solenoid valve inserted as small segments of the analyte solution was sequentially alternated with segments of the NaOH solution for adjusting the medium for the photodegradation. Both flow rates (sample and medium) were adjusted to required time for photodegradation, 90 s; and then, the resulting solution was also sequentially inserted as segments alternated with segments of the oxidizing solution system, hexacyanoferrate (III) in alkaline medium. The calibration range from 1 microg L(…
Volatile dimethylsiloxanes in market seafood and freshwater fish from the Xúquer River, Spain
2016
Abstract Volatile dimethylsiloxanes are a family of synthetic organosilicon-compounds, which have received rising attention because of their widespread use and occurrence in the environment. In the present work, an analytical method based on ultrasound-assisted solid–liquid extraction (USAE) followed by gas chromatography coupled to tandem mass spectrometry (GC–MS/MS) has been optimized and applied to assess the presence of eight volatile dimethyl siloxanes (VMS) (hexamethylcyclotrisiloxane (D3), octamethylcyclotetra-siloxane (D4), decamethylcyclopentasiloxane (D5), dodecamethylcyclohexasiloxane (D6), octamethyltrisiloxane (MDM), decamethyltetrasiloxane (MD2M) and dodecamethylpentasiloxane …
Immuno-SLM—a combined sample handling and analytical technique
2004
Immuno-supported liquid membrane (immuno-SLM) extraction is a new technique that makes use of antibody (Ab)-antigen interactions as the "extraction force" to drive the mass transfer in a selective way. In immuno-SLM, anti-analyte (Ag) Abs are introduced into the acceptor phase of the SLM unit to trap the Ag that passes from the flowing donor through the SLM into the stagnant acceptor. The amount of immuno-extracted analyte (AbAg) is quantified by connecting the immuno-SLM unit on-line with a non-competitive heterogeneous fluorescence flow immunoassay (FFIA) that makes use of a fluorescein-labeled analyte tracer that titrates the residual excess of Ab present in the acceptor. A restricted ac…