Search results for "Fucolectin"

showing 2 items of 2 documents

A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax

2001

A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37 degrees C. Two protein components were found in the hemagglutinating fractions eluted from the Sepharose column. Only the 34 kDa component (DLL2) eluted from the polyacrylamide gels (SDS-PAGE) showed agglutinating activity against rabbit erythrocytes. SDS-PAGE, in non-reducing conditions, revealed a single 66 kDa protein that reacted with antibodies to the 34 kDa component. Therefore, a dimeric structure stabilized by disulfide bonds can be…

SerumImmunoblottingCarbohydratesSettore BIO/05 - ZoologiaBiophysicsHemagglutinin (influenza)CentrifugationBiochemistryFucoseSepharosechemistry.chemical_compoundAffinity chromatographyLectinsAnimalsDicentrarchus labraxHemagglutininSea bassMolecular BiologybiologyLectinHemagglutination Testsbiology.organism_classificationFishchemistryBiochemistryGalactosebiology.proteinBassElectrophoresis Polyacrylamide GelDicentrarchusFucolectinBiochimica et Biophysica Acta (BBA) - General Subjects
researchProduct

F-Type Lectins: A highly diversified family of fucose-binding proteins with a unique sequence motif and structural fold, involved in self/non-self-re…

2017

The F-type lectin (FTL) family is one of the most recent to be identified and structurally characterized. Members of the FTL family are characterized by a fucose recognition domain [F-type lectin domain (FTLD)] that displays a novel jellyroll fold (“F-type” fold) and unique carbohydrate- and calcium-binding sequence motifs. This novel lectin family comprises widely distributed proteins exhibiting single, double, or greater multiples of the FTLD, either tandemly arrayed or combined with other structurally and functionally distinct domains, yielding lectin subunits of pleiotropic properties even within a single species. Furthermore, the extraordinary variability of FTL sequences (isoforms) th…

lcsh:Immunologic diseases. Allergy0301 basic medicineGene isoformImmunologySettore BIO/05 - ZoologiaFucose bindingReviewFucoseF-type lectinsSelf/non-self-recognitionKelch motif03 medical and health scienceschemistry.chemical_compoundGene duplicationImmunology and AllergyStructural modelingGeneticsInnate immunitybiologyPhylogenetic treefucolectinsLectinGlycan recognition030104 developmental biologychemistrybiology.proteinFucose-bindingFucolectinlcsh:RC581-607Sequence motifF-type lectinF-type lectins; Fucolectins; Fucose-binding; Glycan recognition; Innate immunity; Self/non-self-recognition; Structural modeling; Immunology and Allergy; Immunology
researchProduct