Search results for "GENETICA"
showing 10 items of 906 documents
PTHrP in differentiating human mesenchymal stem cells: Transcript isoform expression, promoter methylation, and protein accumulation
2013
Human PTHrP gene displays a complex organization with nine exons producing diverse mRNA variants due to alternative splicing at 5' and 3' ends and the existence of three different transcriptional promoters (P1, P2 and P3), two of which (P2 and P3) contain CpG islands. It is known that the expression of PTHrP isoforms may be differentially regulated in a developmental stage- and tissue-specific manner. To search for novel molecular markers of stemness/differentiation, here we have examined isoform expression in fat-derived mesenchymal stem cells both maintained in stem conditions and induced toward adipo- and osteogenesis. In addition, the expression of the splicing isoforms derived from P2 …
Perfluorocarbons–graphene oxide nanoplatforms as biocompatible oxygen reservoirs
2018
Abstract 3-Pentadecafluoroheptyl,5-perfluorophenyl-1,2,4-oxadiazole (FOX) molecules were attached onto a graphene oxide (GO) via a facile aromatic substitution in alkaline environment. This approach allows achieving high degree of functionalization under mild conditions. The covalent attachment of perfluoromoieties onto GO lamellae was confirmed by spectroscopic analyses. The performance of these nanoplatforms (GOF) as oxygen reservoirs was assessed at different concentrations and temperature. The results revealed that under physiologic conditions GO and FOX synergistically operate for increasing oxygen uptake and release, either from a thermodynamic and a kinetic point of view. Even at low…
GENETICA GENERALE
2020
Genetica formale Metodo e prove sperimentali di Mendel Caratteri singoli e segregazione, Caratteri e assortimento indipendente, Esperienze mendeliane "ieri ed oggi", I Leggi di Mendel, I Caratteri mendeliani e reincrocio IGenetica "oltre" Mendel Dominanza incompleta Codominanza Significato e valore della dominanza e della recessività Alleila multipla Pleiotropia Interazione tra geni Alleli letali Linkage: esperienze di Morgan e associazione genica Associazione completa e associazione incompleta Basi biologiche della ricombinazione Complesso sinaptonemale, rotture a doppio filamento e crossing over Mappe fisiche e mappe genetiche Ambiente e geni L'ambiente e l'espressione dei geni: penetranz…
"Gustare" la genetica
2017
Siamo diversi o siamo uguali? Questa apparentemente banale domanda posta a soggetti non esperti di biologia e genetica ha tendenzialmente sempre dato una risposta a favore dell’uguaglianza. Molte delle pratiche mediche sono state consolidate negli anni, sia in tipologia che in posologia, sull’assioma dell’uguaglianza di effetti e di controindicazioni che farmaci somministrati potevano dare ad un insieme di soggetti. Anche determinati stili di vita sono, in un primo momento, misurati da chiunque sull’assioma dell’uguaglianza: “se chi fuma non sta male, il fumo non farà male neanche a me” era il sillogismo in vigore fino agli anni 90.La percezione del gusto, nei vertebrati e nella specie uman…
Molecular studies on the toxifying effects by genetically engineered cytochromes P450.
1999
After almost two decades, it is now evident that methodology based on molecular biology and gene technology has dramatically changed the way basic and applied toxicology is being performed. It star...
Reduced levels of membrane-bound alkaline phosphatase in Vip3Aa-resistant Heliothis virescens
2020
ABSTRACTThe Vip3Aa insecticidal protein fromBacillus thuringiensis(Bt) is produced by specific transgenic corn and cotton varieties for efficient control of target lepidopteran pests. The main threat to this technology is the evolution of resistance in targeted insect pests, thus understanding the mechanistic basis of resistance is crucial to deploy the most appropriate strategies for resistance management. In this work, a laboratory-selected colony ofHeliothis virescens(Vip-Sel) highly resistant to the Vip3Aa protein was used to test whether an alteration of membrane receptors in the insect midgut might explain the resistance phenotype. Binding of125I-labeled Vip3Aa to brush border membran…
Resistant management of Bt corn and sustainability in Italy
2006
The Resistant Management (RM) of Bt corn in US requires 20% refuge area planted with non transgenic maize to preserve susceptible individuals of the pest. In Europe, especially in Italy, different considerations are necessary when RM plans are to be applied. In fact, when determining the size of the refuge areas other aspects around the transgenic corn, such as the surface of co-existing plans and other crops, must be considered. ECB is a polyphagous insect and its biology must be taken into account in the RM, especially in Italy where several crops and natural areas exist close to each other, and where field surfaces are clearly smaller than in the US. An important aspect of RM is the dete…
Novel NG2-CreERT2 knock-in mice demonstrate heterogeneous differentiation potential of NG2 glia during development
2014
NG2 (nerve/glia antigen-2) is a type I transmembrane glycoprotein and also known as chondroitin sulfate proteoglycan 4. In the parenchyma of the central nervous system, NG2-expressing (NG2(+) ) cells have been identified as a novel type of glia with a strong potential to generate oligodendrocytes (OLs) in the developing white matter. However, the differentiation potential of NG2 glia remained controversial, largely attributable to shortcomings of transgenic mouse models used for fate mapping. To minimize these restrictions and to more faithfully mimic the endogenous NG2 expression in vivo, we generated a mouse line in which the open reading frame of the tamoxifen-inducible form of the Cre D…
Safe and Effective Adoptive T-Cell Receptor Transfer with a High Affinity Single Chain p53(264–272)-Specific TCR
2012
Abstract Abstract 4226 Several studies have demonstrated the clinical efficacy of adoptive T cell therapy for targeting cancer. Using HLA-A2.1 transgenic mice, we have demonstrated the feasibility of T-cell receptor (TCR) gene transfer into T cells to circumvent self-tolerance to the widely expressed human p53(264–272) tumor-associated antigen and developed approaches to generate high-affinity CD8-independent TCR. A safety concern of TCR gene transfer is the pairing of endogenous and introduced TCR chains resulting in the potential generation of self-reactive T cells (off-target autoimmunity). Several strategies to favor matched TCR chains pairing and thus enhancing TCR cell surface express…
Cutting Edge: TGF-β Signaling Is Required for the In Vivo Expansion and Immunosuppressive Capacity of Regulatory CD4+CD25+ T Cells
2004
Abstract Data regarding the role of TGF-β for the in vivo function of regulatory CD4+CD25+ T cells (Treg) are controversial. A transgenic mouse model with impaired TGF-β signaling specifically in T cells was used to assess the role of endogenous TGF-β for the in vivo function of CD4+CD25+ Treg in a murine model of colitis induced by dextran sulfate. Transfer of wild-type, but not transgenic CD4+CD25+ Treg was found to suppress colitis in wild-type mice. In addition, by transferring CFSE-labeled CD4+CD25+ Treg we could demonstrate that endogenous TGF-β promotes the expansion of CD4+CD25+ Treg in vivo. Transgenic mice themselves developed reduced numbers of peripheral CD4+CD25+ Treg and were …