Search results for "GENI"
showing 10 items of 6843 documents
Genetic analysis of familial Alzheimer’s disease, primary lateral sclerosis and paroxysmal kinesigenic dyskinesia: a tool to uncover common mechanist…
2021
Tesis doctoral 270 p. figuras y tablas
IL PADRE E LA COSTRUZIONE DI UNA NUOVA IDENTITÀ GENITORIALE NELL’ITALIA DEGLI ANNI CINQUANTA
2016
La società tardo-moderna viene considerata una società senza padri: da un lato si denuncia la morte o l’eclisse del padre, dall’altro lato si auspica un suo ritorno. Prendendo l’avvio dalla contemporaneità, l’articolo analizza la fine della paternità come un fenomeno di lunga durata che riguarda la crisi del codice paterno autoritario e ne ricerca le radici nel piano della ricerca storica soffermandosi sull’Italia degli anni Cinquanta. Attraverso fonti audiovisive e letterarie viene ricostruita un’identità paterna che non sembra corrispondere a quella maggiormente diffusa nell’immaginario collettivo: essa non appare rassicurante, decisa, autoritaria, ma fragile, ambigua e da decifrare. Si t…
Unioni civili, matrimoni omosessuali e diritto alla genitorialità. Una prospettiva pedagogica
2014
Testing for Lynch Syndrome in Endometrial Carcinoma: From Universal to Age-Selective MLH1 Methylation Analysis
2022
Simple Summary International guidelines recommend universal screening of endometrial carcinoma patients for Lynch syndrome, a hereditary cancer predisposition syndrome. Screening is based on mismatch repair protein immunohistochemistry and reflex MLH1 methylation analysis to exclude the likely sporadic cases of MMR deficiency. As sporadic MLH1 protein loss is common in endometrial carcinoma, the ability to target methylation testing would save efforts and costs. We discovered that limiting methylation testing to patients under 65 years would have significantly reduced the testing effort while maintaining a low false negative rate for MLH1-LS detection (0% and 3% in our clinic and registry-b…
Piederības vietniekvārdi un to lietojums latviešu valodā
2017
Bakalaura darbā ir aplūkoti piederības vietniekvārdi, to atvasinājumi ar -ēj- un personas vietniekvārdu ģenitīvs piederības vietniekvārdu funkcijā. Šī pētījuma uzdevumi ir teorētisko materiālu vākšana, rakstveida un mutvārdu saziņas piemēru apkopošana un analizēšana, pētot šo vietniekvārdu funkciju teikumā. Bakalaura darba mērķis ir analizēt piederības vietniekvārdu funkciju teikumā un izprast to lietojumu latviešu valodā. Šī pētījuma nolūkos ir savākti aptuveni tūkstoš piemēru, kuri atspoguļo piederības vietniekvārdu lietojumu latviešu valodā. Bakalaura darbā analizētie piemēri ir no “Līdzsvarota mūsdienu latviešu valodas tekstu korpusa”, dažādiem ziņu portāliem, kā arī pašas autores fiksē…
Induction of body weight loss through RNAi-knockdown of APOBEC1 gene expression in transgenic rabbits
2014
In the search of new strategies to fight against obesity, we targeted a gene pathway involved in energy uptake. We have thus investigated the APOB mRNA editing protein (APOBEC1) gene pathway that is involved in fat absorption in the intestine. The APOB gene encodes two proteins, APOB100 and APOB48, via the editing of a single nucleotide in the APOB mRNA by the APOBEC1 enzyme. The APOB48 protein is mandatory for the synthesis of chylomicrons by intestinal cells to transport dietary lipids and cholesterol. We produced transgenic rabbits expressing permanently and ubiquitously a small hairpin RNA targeting the rabbit APOBEC1 mRNA. These rabbits exhibited a moderately but significantly reduced …
Análisis poblacional y filogeográfico de vectores de la enfermedad de Chagas en Perú basado en análisis multigénicos del ADN ribosomal y mitocondrial
2017
La enfermedad de Chagas, cuyo agente causal es Trypanosoma cruzi, constituye un grave problema de salud pública en toda Latinoamérica. La principal forma de transmisión es la vectorial, de la que son responsables hemípteros redúvidos de la subfamilia Triatominae adaptados a los hábitats doméstico y peridoméstico. Puesto que el parásito se mantiene en un ciclo zoonótico silvestre y es imposible de eliminar, la principal forma de lucha contra la transmisión vectorial consiste en la eliminación de las poblaciones domiciliadas del vector. Los estudios moleculares constituyen una herramienta de gran utilidad a la hora de planificar las campañas de control vectorial, puesto que permiten conocer y…
Fight evolution with evolution: plasmid‐dependent phages with a wide host range prevent the spread of antibiotic resistance
2013
The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever antibiotics are applied, the genes encoding for antibiotic resistance are selected for within bacterial populations. This has led to the prevalence of conjugative plasmids that carry resistance genes and can transfer themselves between diverse bacterial groups. In this study, we investigated whether it is feasible to attempt to prevent the spread of antibiotic resistances with a lytic bacteriophage, which can replicate in a wide range of gram‐negative bacteria harbouring conjugative drug resistance–conferring plasmids. The counter‐selection against the plasmid was sh…
In Vitro Cytotoxic Effect of Aqueous Extracts from Leaves and Rhizomes of the Seagrass Posidonia oceanica (L.) Delile on HepG2 Liver Cancer Cells: Fo…
2023
Aqueous extracts from Posidonia oceanica’s green and brown (beached) leaves and rhizomes were prepared, submitted to phenolic compound and proteomic analysis, and examined for their potential cytotoxic effect on HepG2 liver cancer cells in culture. The chosen endpoints related to survival and death were cell viability and locomotory behavior, cell-cycle analysis, apoptosis and autophagy, mitochondrial membrane polarization, and cell redox state. Here, we show that 24 h exposure to both green-leaf- and rhizome-derived extracts decreased tumor cell number in a dose–response manner, with a mean half maximal inhibitory concentration (IC50) estimated at 83 and 11.5 μg of dry extract/mL, respecti…
Early evolutionary colocalization of the nuclear ribosomal 5S and 45S gene families in seed plants: evidence from the living fossil gymnosperm Ginkgo…
2012
In seed plants, the colocalization of the 5S loci within the intergenic spacer (IGS) of the nuclear 45S tandem units is restricted to the phylogenetically derived Asteraceae family. However, fluorescent in situ hybridization (FISH) colocalization of both multigene families has also been observed in other unrelated seed plant lineages. Previous work has identified colocalization of 45S and 5S loci in Ginkgo biloba using FISH, but these observations have not been confirmed recently by sequencing a 1.8 kb IGS. In this work, we report the presence of the 45S–5S linkage in G. biloba, suggesting that in seed plants the molecular events leading to the restructuring of the ribosomal loci are much o…