Search results for "GLYCOGEN"

Effect of sublethal exposure to mercury in the biochemical composition of hepatopancreas in Procambarus clarkii during the recovery after starvation

1993

Abstract Freshwater crayfish undergo seasonal periods of starvation. The hepatopancreas is the main energetic-storage organ in this animal. An adequate replenishment of energetic reserves after these periods of fasting is critical for the proper growth and reproduction in this species. Mercury compounds enter the biological environment from natural discharges and anthropogenic sources. Mercuric chloride is one of the most toxic metallic salts found in polluted waters. In these studies, we report the effect of mercuric chloride in the biochemical composition of crayfish hepatopancreas during the recovery period after prolonged starvation. Adult intermolt crayfish (P. clarkii) were kept starv…

Absence of correlation between oxysterol accumulation in lipid raft microdomains, calcium increase, and apoptosis induction on 158N murine oligodendr…

2013

There is some evidence that oxidized derivatives of cholesterol, 7-ketocholesterol (7KC) and 7β-hydroxycholesterol (7βOHC), are increased in the plasma of patients with neurodegenerative diseases associated with demyelinization of the central nervous system (CNS). It was therefore of interest to investigate the effects of these oxysterols on oligodendrocytes, the myelin-forming cells in the CNS. To this end, 158N murine oligodendrocytes were treated with 7KC or 7βOHC inducing an apoptotic mode of cell death characterized by condensation/fragmentation of the nuclei, dephosphorylation of Akt and GSK3, mitochondrial depolarization involving Mcl-1, and caspase-3 activation. In contrast, under t…

α-Tocopherol impairs 7-ketocholesterol-induced caspase-3-dependent apoptosis involving GSK-3 activation and Mcl-1 degradation on 158N murine oligoden…

2011

Abstract In important and severe neurodegenerative pathologies, 7-ketocholesterol, mainly resulting from cholesterol autoxidation, may contribute to dys- or demyelination processes. On various cell types, 7-ketocholesterol has often been shown to induce a complex mode of cell death by apoptosis associated with phospholipidosis. On 158N murine oligodendrocytes treated with 7-ketocholesterol (20 μg/mL corresponding to 50 μM, 24–48 h), the induction of a mode of cell death by apoptosis characterised by the occurrence of cells with condensed and/or fragmented nuclei, caspase activation (including caspase-3) and internucleosomal DNA fragmentation was observed. It was associated with a loss of tr…

Persistent Tachypnea of Infancy. Usual and Aberrant.

2016

Persistent tachypnea of infancy (PTI) is a specific clinical entity of undefined etiology comprising the two diseases neuroendocrine cell hyperplasia of infancy (NEHI) and pulmonary interstitial glycogenosis. The outcome of typical NEHI is favorable. The outcome may be different for patients without a typical NEHI presentation, and thus a lung biopsy to differentiate the diseases is indicated.To determine whether infants with the characteristic clinical presentation and computed tomographic (CT) imaging of NEHI (referred to as "usual PTI") have long-term outcome and biopsy findings similar to those of infants with an aberrant presentation and/or with additional localized minor CT findings (…

Cooperation of Two mRNA-Binding Proteins Drives Metabolic Adaptation to Iron Deficiency

2008

Summary Iron (Fe) is an essential cofactor for a wide range of cellular processes. We have previously demonstrated in yeast that Cth2 is expressed during Fe deficiency and promotes degradation of a battery of mRNAs leading to reprogramming of Fe-dependent metabolism and Fe storage. We report here that the Cth2-homologous protein Cth1 is transiently expressed during Fe deprivation and participates in the response to Fe deficiency through the degradation of mRNAs primarily involved in mitochondrially localized activities including respiration and amino acid biosynthesis. In parallel, wild-type cells, but not cth1 Δ cth2 Δ cells, accumulate mRNAs encoding proteins that function in glucose impo…

A human multisystem disorder with autoinflammation, leukoencephalopathy and hepatopathy is caused by mutations in C2orf69

2021

AbstractBackgroundDeciphering the function of the many genes previously classified as uncharacterized “open reading frame” (orf) completes our understanding of cell function and its pathophysiology.MethodsWhole-exome sequencing, yeast 2-hybrid and transcriptome analyses together with molecular characterization are used here to uncover the function of the C2orf69 gene.ResultsWe identify loss-of-function mutations in the uncharacterized C2orf69 gene in eight individuals with brain abnormalities involving hypomyelination and microcephaly, liver dysfunction and recurrent autoinflammation. C2orf69 contains an N-terminal signal peptide that is required and sufficient for mitochondrial localizatio…

Response of yeast cells to high glucose involves molecular and physiological differences when compared to other osmostress conditions.

2015

Yeast cells can be affected by several causes of osmotic stress, such as high salt, sorbitol or glucose concentrations. The last condition is particularly interesting during natural processes where this microorganism participates. Response to osmostress requires the HOG (High Osmolarity Glycerol) pathway and several transcription factors, including Hot1, which plays a key role in high glucose concentrations. In this work, we describe how the yeast response to osmotic stress shows differences in accordance with the stress agent responsible for it. Compared with other conditions, under high glucose stress, delocalization of MAPK (Mitogen-Activated Protein Kinase) Hog1 is slower, induction of …

Pesticide Effects on Eel Metabolism

1995

Previous works on endosulfan eel toxicology in this laboratory demonstrated that 0.041 mg/liter of endosulfan was the 50% lethal concentration of 96 hr exposure. Eels of species Anguilla anguilla were exposed to two sublethal endosulfan concentrations: 8.2 μg/liter (1/5 LC50) and 4.1 μg/liter (1/10 LC50), and the experiment was done at different exposure times: 12, 24, 48, 72, and 96 hr. Muscle glycogen content decreased significantly (P < 0.05) at 24, 48, 72, and 96 hr exposure to 8.2 μg/liter pesticide; levels did not decline at any time when animals were exposed to 4.1 μg/liter. Muscle lactate levels in fish did not change significantly while lactate levels in eel blood increased at 12, …

Lysosomal and Peroxisomal Disorders. Recent Advances: Introduction

2006

Oxidative stress, a new hallmark in the pathophysiology of Lafora progressive myoclonus epilepsy

2015

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