Search results for "GPC"

Human taste receptors : study of structure-function relationships

2019

Sweet, umami and bitter taste detectors are membrane receptors that belong to the family of G-protein coupled receptors (GPCRs). They are characterized by the existence of a hydrophobic transmembrane domain (TMD) and an activation mechanism that involves a heterotrimeric G protein.Human has 25 bitter taste receptors TAS2R. These receptors belong to class A GPCRs. Their architecture consists of a TMD structured in 7 -helix which form the orthosteric binding site of bitter molecules. The umami taste receptor is a heterodimer composed of the TAS1R1 and TAS1R3 subunits, while the TAS1R2 and TAS1R3 subunits form the sweet taste receptor. Each subunits belongs to the class C GPCRs and shares a c…

Identification of GPR23/LPA4 as a candidate G protein-coupled receptor for Guanosine

2012

Guanosine-Induced Antiproliferative Effects Are Modulated by GPCR Expression in Human Glioma and Melanoma Cell Lines.

2012

Guanosine-Induced Antiproliferative Effects Are Modulated by GPCR Expression in Human Glioma and Melanoma Cell Lines.

Origin of neuronal-like receptors in Metazoa: cloning of a metabotropic glutamate/GABA-like receptor from the marine sponge Geodia cydonium.

1999

To date, no conclusive evidence has been presented for the existence of neuronal-like elements in Porifera (sponges). In the present study, isolated cells from the marine sponge Geodia cydonium are shown to react to the excitatory amino acid glutamate with an increase in the concentration of intracellular calcium [Ca2+]i. This effect can also be observed when the compounds L-quisqualic acid (L-QA) or L-(+)-2-amino-4-phosphonobutyric acid (L-AP-4) are used. The effect of L-QA and L-AP-4, both agonists for metabotropic glutamate receptors (mGluRs), can be abolished by the antagonist of group I mGluRs, (RS)-alpha-methyl-4-carboxyphenylglycine. These data suggest that sponge cells contain an mG…

Understanding and Controlling Food Protein Structure and Function in Foods: Perspectives from Experiments and Computer Simulations

2020

The structure and interactions of proteins play a critical role in determining the quality attributes of many foods, beverages, and pharmaceutical products. Incorporating a multiscale understanding of the structure–function relationships of proteins can provide greater insight into, and control of, the relevant processes at play. Combining data from experimental measurements, human sensory panels, and computer simulations through machine learning allows the construction of statistical models relating nanoscale properties of proteins to the physicochemical properties, physiological outcomes, and tastes of foods. This review highlights several examples of advanced computer simulations at mol…

Analisi della affidabilità dei database pluviometrici globali a scala locale. Applicazione al territorio siciliano

2012

Negli ultimi anni i sistemi di misurazione e di stima delle precipitazioni, sono stati arricchiti dalla possibilità di ottenere stime attraverso dati acquisiti da sensori satellitari. I prodotti di stima così ottenuti risultano comunque spesso fortemente distorti. Per tale ragione le stime sulla base di soli dati satellitari, vengono ulteriormente elaborate attraverso l'utilizzo di dataset che raccolgono dati di precipitazione provenienti da reti pluviografiche al fine di ridurre la distorsione che caratterizza i dati satellitari. È auspicabile che questi dataset siano quanto più precisi al fine di fornire dati adatti alla correzione dei dati satellitari e in particolare alla riduzione dell…

Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor

2012

Facteur d'impact (5 ans) : 1,617Notoriété à 2 ans : Acceptable (biochem.res.methods); The sweet taste receptor is a heterodimeric receptor composed of the T1R2 and T1R3 subunits, while T1R1 and T1R3 assemble to form the umami taste receptor. T1R receptors belong to the family of class C G-protein coupled receptors (GPCRs). In addition to a transmembrane heptahelical domain, class C GPCRs have a large extracellular N-terminal domain (NTD), which is the primary ligand-binding site. The T1R2 and T1R1 subunits have been shown to be responsible for ligand binding, via their NTDs. However, little is known about the contribution of T1R3-NTD to receptor functions. To enable biophysical characteriza…

Interactions between cholinergic and fibroblast growth factor receptors in brain trophism and plasticity

2014

Acetylcholine, acting on both nicotinic receptors (nAChRs) and muscarinic receptors (mAChRs), plays a role in the regulation of synaptic plasticity, being involved in the regulation of cellular processes and cognitive functions, such as learning, memory and attention. Recently, G protein coupled receptors (GPCRs), including mAChRs, have been reported to transactivate tyrosine-kinase receptors (RTK), such as epidermal growth factor receptor (EGFR), and initiate their intracellular signaling. In this minireview we have first analysed the RTK transactivation mechanisms, involving cholinergic receptors, and thereafter the interplay between AChR and neurotrophic factor systems built up by FGF2 a…

Microstructure and rheological response of laboratory-aged SBS-modified bitumens

2019

Polymer additives are widely used to improve the performance of road bitumens including their resistance to hardening during oxidation, although their oxidative inhibitor effect has not been well documented. This study aims to investigate the effect of laboratory-simulated ageing on the microstructure, and rheological properties of Polymer Modified Bitumens (PMBs) prepared with a Styrene-Butadiene-Styrene (SBS) copolymer and sulphur as a cross-linker. The laboratory ageing was conducted through the Rolling Thin Film Oven (RTFO) and the Pressure Aging Vessel (PAV) procedures. The unaged and aged binders were characterised using the Multiple Stress Creep and Recovery (MSCR) test, Gel Permeati…

An efficient Escherichia coli expression system for the production of a functional N-terminal domain of the T1R3 taste receptor.

2012

http://www.landesbioscience.com/; International audience; Sweet taste is mediated by a dimeric receptor composed of two distinct subunits, T1R2 and T1R3, whereas the T1R1/T1R3 receptor is involved in umami taste perception. The T1R1, T1R2, and T1R3 subunits are members of the small family of class C G protein-coupled receptors (GPCRs). The members of this family are characterized by a large N-terminal domain (NTD), which is structurally similar to bacterial periplasmic-binding proteins and contains the primary ligand-binding site. In a recent study, we described a strategy to produce a functional dimeric human T1R3-NTD. Although the protein was expressed as inclusion bodies (IBs) using the …